Elucidation of virulence factors of Leptosrirakirschneriserovargrippotyphosa using comparative genomics.
Stephanie Lepp, JiaxinQu, Fares Najar, and Bruce Roe
Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Ok 73160
Leptospirosis is a zoonotic disease of global significance. In the United States 15-20% of cattle and swine are infected with leptospira, and leptosprosis is the primary cause of abortions and stillbirths in livestock (1?). The disease is a major threat to animal health and the livestock industry. Moreover, leptospirosis can produce deadly infections in accidental hosts such as humans (2,3). Tens of millions of humans become infected each year worldwide, and in some areas the death rate from infection is as high as 25% (4). Because leptospires are difficult to culture in vitro (5) genomic sequence analysis is a powerful tool for studying these pathogens. In this study we used comparative genomics to elucidate virulence factors of Leptosrirakirschneriserovargrippotyphosa. The study revealed that Leptosrirakirschneri might utilize lipopolysacharide as an endotoxin to elicit immune response since the metabolic pathway for LPS synthesis is complete. Additionally, outer membrane proteins that may enable Leptosrira to adhere to host cell surfaces were identified. The study also revealed the presence of motility and chemotaxis proteins that might allow Leptosrira to penetrate host tissues rapidly. The use of in silico analysis in recognizing virulence factors in various leptospiraserovars is an important complementary tool to biochemical methods as it aids in formulating hypothesis for molecular and biochemical tests which ultimately will help generate vaccines and other drugs to fight Leptospirosis.
Enter through cut or mucosal membrane8
Leptospira evades immune response
Quickly enter lymphatic and circulatory system9
Attach to epithelial cells, in liver, kidney, lungs, heart etc.9
Evoke immune response causing major inflammatory problems8,9.
Toxin mediated capillary vasculitis8 and jaundice9.
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