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Interrogating viral capsid assembly with ion mobility-mass spectrometry

Interrogating viral capsid assembly with ion mobility-mass spectrometry. Charlotte Uetrecht , Ionoa M. Barbu , Glen K. Shoemaker, Esther van Duijn , and Albert J. R. Heck Nature Chemistry 2010. Previous Understanding. Poliovirus crystal structure. Flavivirus cryo -EM model.

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Interrogating viral capsid assembly with ion mobility-mass spectrometry

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  1. Interrogating viral capsid assembly with ion mobility-mass spectrometry Charlotte Uetrecht, Ionoa M. Barbu, Glen K. Shoemaker, Esther van Duijn, and Albert J. R. Heck Nature Chemistry 2010

  2. Previous Understanding Poliovirus crystal structure Flaviviruscryo-EM model Liljaset al. Textbook of StructBiol(2008)

  3. Unresolved Questions on Viral Assembly • General mechanisms for proteins assembling into complexes • Intermediates – structures and intermediates

  4. IM-MS • Ion mobility mass spectrometry (IMS) combined with native mass spectrometry (MS) • Norovirus self-assembly • Hepatitis B vrius (HBV) self-assembly

  5. Mass Spectra of Capsid Intermediates Norovirus HBV Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  6. Isolating Subunits T= 1 3 4 7 13 Liljaset al. Textbook of StructBiol(2008)

  7. HBV Oligomers Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  8. Norovirus Oligomers Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  9. Structural Characterization Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  10. HBV Oligomers Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  11. Capsid Assembly Uetrechtet al.Nat Chem, 3(2), 126-132 (2011).

  12. Future Directions

  13. A foundation for understanding CRISPR mechanisms Sontheimer, Erik J., & Marraffini, Luciano A. (2010). Microbiology: Slicer for DNA. Nature, 468(7320), 45-46. van Duijn E et al. Mol Cell Proteomics 2012;11:1430-1441

  14. Mass spectrometry: come of age for structural and dynamical biology Benesch JL and Ruotolo BT. CurrOpinStruct Biol. 2011 October; 21(5): 641–649.

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