Composition of the blood. The circulating blood is composed of plasma and cells. The cells are red cells (or erythrocytes), white cells (or leucocytes) and platelets . Blood cells can be identified in blood films stained with a mixture of basic and acidic dyes.
The relative percentage of each type of white blood cells in peripheral blood.
This experiment is a part of blood routine test.
Observe one field and record the number of WBC according to the different type then turn to another field in the snake-liked direction
*avoid repeat or miss some cells
Cytoplasm : pink
Granules : primary &
Nucleus : dark purple blue
dense chromatinStab neutrophil
non-segmented nuclei. They are referred to as neutrophil band cells or band forms. They are less mature than segmented neutrophils.
Cytoplasm: very pale blue
Nucleus: dark purple blue
dense heterogeneous chromatin
2-5 lobesSegmented neutrophil
the acidic and the basic components of the stain
Cytoplasm : full of granules
Granules: large retractile orange-red
2 lobes like a pair of glassEosinophil
Granules: dark blue –black obscure nucleus
Cytoplasm : rim, clear, pale blue
Granules: small (a granular)
large (a variable number of azurophilic pinkish-purple granules)
Nucleus: dark blue \round
dense homogeneous chromatinLymphocyte
Diameter: 14-20( divided into small , largeare the largest normal blood cells)
Cytoplasm: grey blue
Granules: dust-like lilac color granules(purple)
large irregularly shaped and foldedMonocyte
Commonly used evacuated tubes divided into small , large
(with the corresponding color coding and additive)