Composition of the blood
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Composition of the blood. The circulating blood is composed of plasma and cells. The cells are red cells (or erythrocytes), white cells (or leucocytes) and platelets . Blood cells can be identified in blood films stained with a mixture of basic and acidic dyes.

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Composition of the blood
Composition of the blood

  • The circulating blood is composed of plasma and cells.

  • The cells are red cells (or erythrocytes), white cells (or leucocytes) and platelets.

  • Blood cells can be identified in blood films stained with a mixture of basic and acidic dyes.

  • Normal white cells are divided into polymorphonuclear leucocytes (or granulocytes) and mononuclear cells.



Definition
Definition

The relative percentage of each type of white blood cells in peripheral blood.

This experiment is a part of blood routine test.


Microscopic exam
Microscopic exam

  • 10× (low fold): overall smear quality, rouleaux, agglutination or parasites

  • 100× (oil Len): WBC Diff, RBC morphology



Observing direction:

Observe one field and record the number of WBC according to the different type then turn to another field in the snake-liked direction

*avoid repeat or miss some cells


Morphology of wbc in peripheral blood
Morphology of WBC in peripheral blood



White blood cells
White blood cells

  • There are three types of granulocyte named according to their staining characteristics in blood films. They are neutrophils,eosinophils and basophils.

  • Mononuclear cells are divided into lymphocytes and monocytes.


Stab neutrophil

Diameter:12-16

Cytoplasm : pink

Granules : primary &

secondary

Nucleus : dark purple blue

dense chromatin

Stab neutrophil


Band form neutrophils
Band form Neutrophils

  • There are smaller numbers of cells of neutrophil lineage with

    non-segmented nuclei. They are referred to as neutrophil band cells or band forms. They are less mature than segmented neutrophils.

  • An increased number of band cells is referred to as a left shift'.


Segmented neutrophil

Diameter: 12-16

Cytoplasm: very pale blue

Granules: primary

secondary

lilac-staining (purple)

Nucleus: dark purple blue

dense heterogeneous chromatin

2-5 lobes

Segmented neutrophil


Neutrophils
Neutrophils

  • The neutrophils in the circulating blood are mainly mature segmented neutrophils.

  • neutrophilic because they owe their colour to uptake of both

    the acidic and the basic components of the stain


Eosinophil

Diameter: 14-16

Cytoplasm : full of granules

Granules: large retractile orange-red

Nucleus: blue

dense chromatin

2 lobes like a pair of glass

Eosinophil


Basophil

Diameter: 14-16

Cytoplasm: pink

Granules: dark blue –black obscure nucleus

Nucleus: blue

Basophil


Lymphocyte

Diameter: small 7-9

large 12-16

Cytoplasm : rim, clear, pale blue

Granules: small (a granular)

large (a variable number of azurophilic pinkish-purple granules)

Nucleus: dark blue \round

dense homogeneous chromatin

Lymphocyte



Normal lymphocytes
Normal divided into small , largelymphocytes

  • Lymphocytes are the smallest WBC.

  • They have large condensed nucleus, with a scanty pale blue cytoplasm.


Monocyte

Diameter: 14-20( divided into small , largeare the largest normal blood cells)

Cytoplasm: grey blue

Granules: dust-like lilac color granules(purple)

Nucleus: blue

large irregularly shaped and folded

Monocyte


Normal monocyte
Normal monocyte divided into small , large

  • Monocytes are the largest WBC.

  • The nucleus is slightly indented .

  • The cytoplasm is abundant, sky blue in colour.

  • Some have vacuoles in the cytoplasm.


Commonly used evacuated tubes divided into small , large

(with the corresponding color coding and additive)


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