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SABLE’S SCOPE

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SABLE’S SCOPE. Who We Are. Founded by John R.B. Lighton, Ph.D. 25 years of scientific discovery Tools for understanding – with a respirometry focus Scientists and engineers still active in research Growing at 25% for last 4 years Growing at over 45% in 2011 Fueled by Promethion.

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slide2

SABLE’S SCOPE

Who We Are

  • Founded by John R.B. Lighton, Ph.D.
  • 25 years of scientific discovery
  • Tools for understanding – with a respirometry focus
  • Scientists and engineers still active in research
  • Growing at 25% for last 4 years
  • Growing at over 45% in 2011
  • Fueled by Promethion
slide4

Sable’s Promethion Inspiration

CUSTOMER REQUESTS:

High Throughput

User Friendly

Scalable

No air tight seal

Turnkey Solution

Good Customer Service

Expertise

Customizable Analysis

Fully Traceable Data

No Chemical Desiccants

Accurate

Fully Integrated

Running Wheel

Live-in Cages with Bedding

slide5

MARKET RESEARCH: Competition

The Bad

The Good

  • Small, not live-in cages
  • No bedding in cages
  • Very low flow rates (IACUC)
  • Accuracy concerns
  • No integrated running wheels
  • Widely used and cited
  • Integrated system
  • High Throughput

The Ugly

  • Black box calculations
  • No raw data storage or data traceability
  • Customer service
  • Difficult to use
  • Very complex to set up
slide9

FOOD INTAKE

Food Intake Monitor

  • Universal Load Cell
  • Mouse or Rat hopper

Features

  • 3mg resolution
  • Crumb tray reduces caching
  • Real-time measurement

Advantages

  • Increased accuracy, versatility
  • Fine granularity gives insight into behavioral patterns
slide10

FOOD INTAKE

Raw Feeding Data

  • Stores hopper mass vs. time
  • Recorded to disc at 1Hz

One Feeding Episode

  • Analyze behavior
  • Determine individual feeding episode intake rates
slide11

Summarized Feeding Data

FOOD INTAKE

  • Identify trends and outliers
  • Can zoom into any event
  • Automated uptake analysis
  • Automated meal analysis

One Feeding Episode

  • Analyze behavior
  • Unprecedented detail available – but only if required
slide12

FOOD INTAKE

Processed Feeding Data

  • Outputs cumulative intake vs. time
  • Shows rate of intake, structure
  • Can output binned data
  • Integrates with other cage data
  • Fully traceable

Food Selection

  • Determine food preference
  • Control access to either/both foods
  • Integrates with other cage data
slide14

FOOD ACCESS CONTROL

Access Control Module

  • Access Control Door
  • Connects to mass sensor

Features

  • Computer controlled access to food
  • Available for Mouse or Rat food hoppers
  • Intelligent Obstruction Detection

Advantages

  • Reduced technician interaction
  • Customizable assays (paired/yoked)
slide15

FOOD ACCESS CONTROL

User Friendly Setup

  • Select from 8 preset assays
  • Customize time and duration
  • Individual cage setup

Select Access Control Type

  • Add to your custom assay
  • Unlimited number of variations
slide16

WATER INTAKE

Water Intake Monitor

  • Universal Load Cell
  • Mouse or Rat dispenser models

Features

  • 3 µL resolution
  • Real-time measurement
  • Stainless steel case (not shown) reduces animal interaction
  • Custom sipper tube reduces leakage

Advantages

  • Increased accuracy
  • Increased granularity gives insight into behavioral patterns
  • Use existing sterilizing systems
slide17

WATER INTAKE

Raw Drinking Data

  • Stores bottle mass vs. time
  • Recorded to disc at 1Hz

Processed Drinking Data

  • Analyze behavior
  • Mathematical drift elimination
  • Determine individual episode intake rates
  • EthoScan Compatible
slide18

BODY MASS

Body Mass Monitor

  • Universal load cell
  • Mouse or Rat models available

Features

  • 3mg resolution
  • Real-time measurement

Advantages

  • Highly accurate
  • Provides in-cage enrichment
  • Reduced technician interaction
slide19

BODY MASS

Raw Body Mass Data

  • Stores bracket mass vs. time
  • Recorded to disc at 1Hz
  • Is used about every 15 minutes

Zoomed In

  • Track body mass over time
slide20

BODY MASS

Processed Body Mass Data

  • Stores most recent body mass for each timestamp
  • Synchronized with other data
  • Diurnal cycle clearly visible
slide21

VOLUNTARY EXERCISE

Wheel-M or Wheel-R

  • Mouse or Rat Wheel
  • Magnetic Reed Switch
  • Optional: Wheel Stop

Features

  • Real-time measurement
  • Stainless steel construction
  • Entirely in cage

Advantages

  • Increased granularity gives insight into behavioral patterns
  • Correlates with metabolic data
slide22

VOLUNTARY EXERCISE

Raw Running Wheel Data

  • Stores RPS vs. time
  • Recorded to disc at 1Hz

Processed Running Wheel Data

  • Shows cumulative distance run
  • Can be binned and synchronized with metabolic data
slide23

TOTAL ACTIVITY

BXYZ-M or BXYZ-R

  • XY and Z IR arrays
  • Mounting bracket

Features

  • 0.25cm calculated centroid
  • Real-time measurement
  • Intelligent obstruction detection
  • Rearing captured with Z axis

Advantages

  • Highly accurate
  • Not affected by static obstructions
  • Correlated with metabolic data
slide24

TOTAL ACTIVITY

Activity Analysis

  • Total Activity
  • Total Distance Traveled
  • Rearing
  • Activity associated with in-cage devices
  • In cage position vs. time
  • Customizable assays
  • Position histograms

Activity Comparison

  • WT vs. ob/ob
  • Interaction with FH-MB
  • Time spent time spent near food hopper
slide25

CALORIMETRY

Flow Generators & Gas Analyzers

  • Automated calibration
  • Sequential or continuous monitoring
  • Can group for 4, 8,16, 24, 32 etc. cages
  • Expandable and modular
  • Water vapor dilution correction
  • Integrated O2, CO2, WVP analyzers
  • Pull-mode (negative pressure)
slide26

DATA ANALYSIS

Data Synchronization

  • Overlay metabolic data with other continuously monitored parameters

Further Analysis

  • All data are perfectly synchronized and can be exported to other programs if required (open formats)
slide27

DATA ANALYSIS

Further Data Synchronization

  • Body mass and food intake
  • Body mass and water intake
  • Body mass, food intake, water intake, and wheel revolutions

Advantages

  • Synchronized data yields traceability
  • Real time measurement improves visibility
  • Both enable behavioral monitoring
slide28

DATA ANALYSIS

Multiplexed vs. Continuous

  • Continuous measurements give unprecedented detail
  • Multiplexed measurements are adequate for many research topics
  • You have the choice
slide29

TOTAL ACTIVITY

Activity Analysis (II)

  • Activity within user-defined areas of any shape
  • Regions of interest interactively defined on customizable background
  • Correlate with other data streams as needed
slide30

TOTAL ACTIVITY

Activity Analysis (III)

slide31

TOTAL ACTIVITY

Activity Analysis (IV)

slide38

DATA ANALYSIS

Raw Data Synchronization - Zoomed

  • Body mass (blk), food intake (red), wheel (yel), and water intake (blu)
slide39

ETHOSCAN

Behavioral Monitoring

  • All cage devices continuously monitored (“focal animal”)
  • Interactions sequentially processed in data analysis
  • Output is timestamp, position, duration, amount and activity
slide40

ETHOSCAN

Behavioral Transition Matrix

  • Statistical analysis of interaction with in-cage devices
  • Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column
slide41

ETHOSCAN

Time Budgets

  • Shows percentage of time spent exhibiting a specific behavior
  • Customizable to your cage setup and behaviors you wish to monitor

Ambulatory Budgets

  • Two wild type mice –with and without access to a wheel
  • Note the significant difference in distance travelled
room calorimetry1
ROOM CALORIMETRY
  • Time-consuming to set up and test
  • Just one span per run… But analyzers drift
  • Propane burn validations may require back-calculation of span gases (black box!)
  • Cannot adequately account for water vapor dilution, especially at low WVP
  • System integration difficult
  • Many individual instruments = many failure points and many companies to deal with
  • Little or no expandability or modularity
room calorimetry3
ROOM CALORIMETRY
  • Raw data not preserved, so - Not auditable
  • Comparisons between systems problematic
  • Little emphasis on traceability (pre-Jon Moon)
  • But – traceability alone will not help
  • Also need transparency!
  • Custom “black box” software is proprietary
  • Software is a priori optimized for distinguishing between two activities
room calorimetry4
ROOM CALORIMETRY
  • Highly stable O2, CO2 analyzers required
  • Low noise (for response correction)
  • WV dilution is a problem…
  • Chemicals are impractical…
  • Typically, chilled condensers are used
  • But these only “dry” the air to ~650 Pa WVP
  • At dewpoints < 1 C, they have no effect at all
  • Plus, FiCO2 changes…
room calorimetry5
ROOM CALORIMETRY

FiCO2 changes…

room calorimetry6
ROOM CALORIMETRY +
  • Minimize O2 drift (SSI O2 analyzers)
  • Then, eliminate O2drift by repeated, interleaved FiO2 measurement (PP)
  • For CO2, use interleaved SSI analyzers (PP)
  • For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP)
  • Record all raw data in complete detail
room calorimetry7
ROOM CALORIMETRY +
  • Use computational processing to reassemble the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP)
  • Apply rigorously neutral smoothing and response correction to data
  • All using a traceable, auditable, shareable script
  • Because raw data are stored, any kind of response correction you wish can be applied!
promethion1
PROMETHION
  • Analyzers integrated (< time-lags)
  • All analyzers temperature-controlled
  • Calibration automated
  • Analog signal transfer eliminated
  • System is indefinitely expandable to future sensors, e.g. bicycle W, treadmill speed/elevation, IR activity, accelerometry, body weight, heart rate, body mass, food intake, etc. etc.
promethion room
PROMETHION (room)
  • No complex pressure etc. regulators required
  • Normocapnic, not steno/hypercapnic
  • High resolution analyzers plus background baselining = higher flow rate (> 80 L/min)
  • Leak problems minimized
  • Single manufacturer = easy, single-point support + FUD-less, quick setup!
  • Use for rooms, hoods or masks
promethion2
PROMETHION
  • Push-button easy!
  • Raw data stored at 1 Hz
  • Any analytical protocol can be applied
  • Drill down from processed data to raw data
  • Users can share analytical scripts
promethion3
PROMETHION
  • Additional sensors are in development
  • All sensors are sampled at 1 Hz in perfect synchrony with gas exchange data
  • Opens the door to many new questions
  • Need a feature? Ask!
slide56

METABOLIC SCREENING

PROMETHION

New Horizons

  • Data Rate Changes from a trickle to a fire hose
  • Use as much as you need
  • Fully modular – add features as you need them
  • Automates analysis without losing flexibility and traceability
  • Sable Systems is committed to your success!
slide57

THANK YOU!

Questions?

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