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SABLE’S SCOPE. Who We Are. Founded by John R.B. Lighton, Ph.D. 25 years of scientific discovery Tools for understanding – with a respirometry focus Scientists and engineers still active in research Growing at 25% for last 4 years Growing at over 45% in 2011 Fueled by Promethion.

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SABLE’S SCOPE

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Sable s scope

SABLE’S SCOPE

Who We Are

  • Founded by John R.B. Lighton, Ph.D.

  • 25 years of scientific discovery

  • Tools for understanding – with a respirometry focus

  • Scientists and engineers still active in research

  • Growing at 25% for last 4 years

  • Growing at over 45% in 2011

  • Fueled by Promethion


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SABLE’S GLOBAL INFLUENCE


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Sable’s Promethion Inspiration

CUSTOMER REQUESTS:

High Throughput

User Friendly

Scalable

No air tight seal

Turnkey Solution

Good Customer Service

Expertise

Customizable Analysis

Fully Traceable Data

No Chemical Desiccants

Accurate

Fully Integrated

Running Wheel

Live-in Cages with Bedding


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MARKET RESEARCH: Competition

The Bad

The Good

  • Small, not live-in cages

  • No bedding in cages

  • Very low flow rates (IACUC)

  • Accuracy concerns

  • No integrated running wheels

  • Widely used and cited

  • Integrated system

  • High Throughput

The Ugly

  • Black box calculations

  • No raw data storage or data traceability

  • Customer service

  • Difficult to use

  • Very complex to set up


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METABOLIC SCREENING


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EXISTING CUSTOMERS


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THE CAGE…


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FOOD INTAKE

Food Intake Monitor

  • Universal Load Cell

  • Mouse or Rat hopper

Features

  • 3mg resolution

  • Crumb tray reduces caching

  • Real-time measurement

Advantages

  • Increased accuracy, versatility

  • Fine granularity gives insight into behavioral patterns


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FOOD INTAKE

Raw Feeding Data

  • Stores hopper mass vs. time

  • Recorded to disc at 1Hz

One Feeding Episode

  • Analyze behavior

  • Determine individual feeding episode intake rates


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Summarized Feeding Data

FOOD INTAKE

  • Identify trends and outliers

  • Can zoom into any event

  • Automated uptake analysis

  • Automated meal analysis

One Feeding Episode

  • Analyze behavior

  • Unprecedented detail available – but only if required


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FOOD INTAKE

Processed Feeding Data

  • Outputs cumulative intake vs. time

  • Shows rate of intake, structure

  • Can output binned data

  • Integrates with other cage data

  • Fully traceable

Food Selection

  • Determine food preference

  • Control access to either/both foods

  • Integrates with other cage data


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FOOD INTAKE ANALYSIS


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FOOD ACCESS CONTROL

Access Control Module

  • Access Control Door

  • Connects to mass sensor

Features

  • Computer controlled access to food

  • Available for Mouse or Rat food hoppers

  • Intelligent Obstruction Detection

Advantages

  • Reduced technician interaction

  • Customizable assays (paired/yoked)


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FOOD ACCESS CONTROL

User Friendly Setup

  • Select from 8 preset assays

  • Customize time and duration

  • Individual cage setup

Select Access Control Type

  • Add to your custom assay

  • Unlimited number of variations


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WATER INTAKE

Water Intake Monitor

  • Universal Load Cell

  • Mouse or Rat dispenser models

Features

  • 3 µL resolution

  • Real-time measurement

  • Stainless steel case (not shown) reduces animal interaction

  • Custom sipper tube reduces leakage

Advantages

  • Increased accuracy

  • Increased granularity gives insight into behavioral patterns

  • Use existing sterilizing systems


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WATER INTAKE

Raw Drinking Data

  • Stores bottle mass vs. time

  • Recorded to disc at 1Hz

Processed Drinking Data

  • Analyze behavior

  • Mathematical drift elimination

  • Determine individual episode intake rates

  • EthoScan Compatible


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BODY MASS

Body Mass Monitor

  • Universal load cell

  • Mouse or Rat models available

Features

  • 3mg resolution

  • Real-time measurement

Advantages

  • Highly accurate

  • Provides in-cage enrichment

  • Reduced technician interaction


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BODY MASS

Raw Body Mass Data

  • Stores bracket mass vs. time

  • Recorded to disc at 1Hz

  • Is used about every 15 minutes

Zoomed In

  • Track body mass over time


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BODY MASS

Processed Body Mass Data

  • Stores most recent body mass for each timestamp

  • Synchronized with other data

  • Diurnal cycle clearly visible


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VOLUNTARY EXERCISE

Wheel-M or Wheel-R

  • Mouse or Rat Wheel

  • Magnetic Reed Switch

  • Optional: Wheel Stop

Features

  • Real-time measurement

  • Stainless steel construction

  • Entirely in cage

Advantages

  • Increased granularity gives insight into behavioral patterns

  • Correlates with metabolic data


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VOLUNTARY EXERCISE

Raw Running Wheel Data

  • Stores RPS vs. time

  • Recorded to disc at 1Hz

Processed Running Wheel Data

  • Shows cumulative distance run

  • Can be binned and synchronized with metabolic data


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TOTAL ACTIVITY

BXYZ-M or BXYZ-R

  • XY and Z IR arrays

  • Mounting bracket

Features

  • 0.25cm calculated centroid

  • Real-time measurement

  • Intelligent obstruction detection

  • Rearing captured with Z axis

Advantages

  • Highly accurate

  • Not affected by static obstructions

  • Correlated with metabolic data


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TOTAL ACTIVITY

Activity Analysis

  • Total Activity

  • Total Distance Traveled

  • Rearing

  • Activity associated with in-cage devices

  • In cage position vs. time

  • Customizable assays

  • Position histograms

Activity Comparison

  • WT vs. ob/ob

  • Interaction with FH-MB

  • Time spent time spent near food hopper


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CALORIMETRY

Flow Generators & Gas Analyzers

  • Automated calibration

  • Sequential or continuous monitoring

  • Can group for 4, 8,16, 24, 32 etc. cages

  • Expandable and modular

  • Water vapor dilution correction

  • Integrated O2, CO2, WVP analyzers

  • Pull-mode (negative pressure)


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DATA ANALYSIS

Data Synchronization

  • Overlay metabolic data with other continuously monitored parameters

Further Analysis

  • All data are perfectly synchronized and can be exported to other programs if required (open formats)


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DATA ANALYSIS

Further Data Synchronization

  • Body mass and food intake

  • Body mass and water intake

  • Body mass, food intake, water intake, and wheel revolutions

Advantages

  • Synchronized data yields traceability

  • Real time measurement improves visibility

  • Both enable behavioral monitoring


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DATA ANALYSIS

Multiplexed vs. Continuous

  • Continuous measurements give unprecedented detail

  • Multiplexed measurements are adequate for many research topics

  • You have the choice


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TOTAL ACTIVITY

Activity Analysis (II)

  • Activity within user-defined areas of any shape

  • Regions of interest interactively defined on customizable background

  • Correlate with other data streams as needed


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TOTAL ACTIVITY

Activity Analysis (III)


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TOTAL ACTIVITY

Activity Analysis (IV)


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DATA ANALYSIS


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DATA ANALYSIS


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DATA ANALYSIS


Multiplexing errors more information

Multiplexing errors- more information


Error from multiplexing

Error from multiplexing


Over and underestimates

Over and underestimates


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DATA ANALYSIS

Raw Data Synchronization - Zoomed

  • Body mass (blk), food intake (red), wheel (yel), and water intake (blu)


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ETHOSCAN

Behavioral Monitoring

  • All cage devices continuously monitored (“focal animal”)

  • Interactions sequentially processed in data analysis

  • Output is timestamp, position, duration, amount and activity


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ETHOSCAN

Behavioral Transition Matrix

  • Statistical analysis of interaction with in-cage devices

  • Shows probability of behaviors in the top row happening immediately after the behavior in the leftmost column


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ETHOSCAN

Time Budgets

  • Shows percentage of time spent exhibiting a specific behavior

  • Customizable to your cage setup and behaviors you wish to monitor

Ambulatory Budgets

  • Two wild type mice –with and without access to a wheel

  • Note the significant difference in distance travelled


Room calorimetry

ROOM CALORIMETRY


Room calorimetry1

ROOM CALORIMETRY

  • Time-consuming to set up and test

  • Just one span per run… But analyzers drift

  • Propane burn validations may require back-calculation of span gases (black box!)

  • Cannot adequately account for water vapor dilution, especially at low WVP

  • System integration difficult

  • Many individual instruments = many failure points and many companies to deal with

  • Little or no expandability or modularity


Room calorimetry2

ROOM CALORIMETRY


Room calorimetry3

ROOM CALORIMETRY

  • Raw data not preserved, so - Not auditable

  • Comparisons between systems problematic

  • Little emphasis on traceability (pre-Jon Moon)

  • But – traceability alone will not help

  • Also need transparency!

  • Custom “black box” software is proprietary

  • Software is a priori optimized for distinguishing between two activities


Room calorimetry4

ROOM CALORIMETRY

  • Highly stable O2, CO2 analyzers required

  • Low noise (for response correction)

  • WV dilution is a problem…

  • Chemicals are impractical…

  • Typically, chilled condensers are used

  • But these only “dry” the air to ~650 Pa WVP

  • At dewpoints < 1 C, they have no effect at all

  • Plus, FiCO2 changes…


Room calorimetry5

ROOM CALORIMETRY

FiCO2 changes…


Room calorimetry6

ROOM CALORIMETRY +

  • Minimize O2 drift (SSI O2 analyzers)

  • Then, eliminate O2drift by repeated, interleaved FiO2 measurement (PP)

  • For CO2, use interleaved SSI analyzers (PP)

  • For eliminating WV dilution, don’t scrub/condense – calculate from WVP, BP! (PP)

  • Record all raw data in complete detail


Room calorimetry7

ROOM CALORIMETRY +

  • Use computational processing to reassemble the dry O2 and CO2 data from the interleaved O2, CO2, WVP data, plus BP (PP)

  • Apply rigorously neutral smoothing and response correction to data

  • All using a traceable, auditable, shareable script

  • Because raw data are stored, any kind of response correction you wish can be applied!


Promethion

PROMETHION


Promethion1

PROMETHION

  • Analyzers integrated (< time-lags)

  • All analyzers temperature-controlled

  • Calibration automated

  • Analog signal transfer eliminated

  • System is indefinitely expandable to future sensors, e.g. bicycle W, treadmill speed/elevation, IR activity, accelerometry, body weight, heart rate, body mass, food intake, etc. etc.


Promethion room

PROMETHION (room)

  • No complex pressure etc. regulators required

  • Normocapnic, not steno/hypercapnic

  • High resolution analyzers plus background baselining = higher flow rate (> 80 L/min)

  • Leak problems minimized

  • Single manufacturer = easy, single-point support + FUD-less, quick setup!

  • Use for rooms, hoods or masks


Promethion room1

PROMETHION (room)


Promethion2

PROMETHION

  • Push-button easy!

  • Raw data stored at 1 Hz

  • Any analytical protocol can be applied

  • Drill down from processed data to raw data

  • Users can share analytical scripts


Promethion3

PROMETHION

  • Additional sensors are in development

  • All sensors are sampled at 1 Hz in perfect synchrony with gas exchange data

  • Opens the door to many new questions

  • Need a feature? Ask!


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METABOLIC SCREENING

PROMETHION

New Horizons

  • Data Rate Changes from a trickle to a fire hose

  • Use as much as you need

  • Fully modular – add features as you need them

  • Automates analysis without losing flexibility and traceability

  • Sable Systems is committed to your success!


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THANK YOU!

Questions?


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