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Low t emperature cycle (4-20 o C) . High temperature cycle (10-30 o C). PST-127. PST-100. PST-114. PST-127. PST-127. PST-100. PST-114. PST-127. Seedling. Adult plant. AvS. PI 178759. AvS. PI 178759.

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Low temperature cycle (4-20oC)

High temperature cycle (10-30oC)

PST-127

PST-100

PST-114

PST-127

PST-127

PST-100

PST-114

PST-127

Seedling

Adultplant

AvS

PI 178759

AvS

PI 178759

Supplementary Fig. 1 shows the reactions of PI 178759 tested with three Pst races (PST-100, PST-114 and PST-127) at seedling and adult-plant stages under low (4-20oC) and high (10-30oC) temperature cycles in comparison to AvS.


PI 178759

N1DT1A

N2DT2A

N6DT6A

N1BT1A

N2BT2D

N3BT3D

N3DT3B

N4BT4D

N4DT4B

N5BT5A

N5DT5B

N6BT6D

N7BT7A

N7DT7B

N2AT2D

N3AT3D

N4AT4D

N5AT5D

N6AT6D

N1AT1B

N7AT7B

AvS

RB

CS

SB

A

B

Supplement Fig. 2 Polyacrylamide gels showing resistance gene analog polymorphism (RGAP) markers Xwgp2132 (A) and Xwgp1315 (B) in Chinese Spring (CS), PI 178759, resistant bulk (RB), susceptible bulk (SB), AvS and the 21 nulli-tetrasomic CS lines. The arrows show the absence of both markers in line N7BT7A, indicating their locations on chromosome 7BL.


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