implementation of a device for assessing dosage for platelet glycoprotein inhibitors
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Implementation of a Device for Assessing Dosage for Platelet Glycoprotein Inhibitors. Steven A. Jones, Yuri Lvov, Tianhong Cui, Bruce Walters Sachin Sah, Kanvasri Jonnalagada, Jeremy Klein. PLATELETS. Blood Coagulation Vasoconstriction, vessel repair, immune response

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implementation of a device for assessing dosage for platelet glycoprotein inhibitors
Implementation of a Device for Assessing Dosage for Platelet Glycoprotein Inhibitors

Steven A. Jones, Yuri Lvov, Tianhong Cui,

Bruce Walters

Sachin Sah, Kanvasri Jonnalagada, Jeremy Klein

platelets
PLATELETS
  • Blood Coagulation
  • Vasoconstriction, vessel repair, immune response
  • Defects lead to bleeding disorders
  • Can enhance atherosclerosis, heart attack, unstable angina, diabetes, stroke, eclampsia
what does the cardiologist want
What Does the Cardiologist Want?
  • Glycoprotein Inhibitors (e.g. abciximab).
  • What is the proper dose for this heart patient?
  • Has an appropriate dose been reached?
  • Does this patient need GP Inhibitors?
analysis of need
Analysis of Need
  • "It is not possible to use in vitro assays for more direct measurement of platelet viability and hemostatic function on a regular basis, because they are difficult to perform, expensive, and time-consuming”

Holme et al., Transfusion, V. 38, 1998

the current implementation

Inlet

Outlet

Channel

Plexiglass Plates

PDMS

Nano Coating

The Current Implementation
fabrication of channels
Fabrication of channels

Thermal Oxide

Silicon Substrate

UV Mask

Negative Photoresist

Silicon Oxide

Develop

Etch Silicon Oxide

Etch Silicon to 150 μm

Cast PDMS mold

early images of platelet adhesion on fibrinogen
Early Images of Platelet Adhesion on Fibrinogen

147/s

385/s

2091/s

3990/s

6000/s

7000/s

adhesion patterns at 20 dynes cm 2
Adhesion Patterns at 20 dynes/cm2

Fibronectin

Fibrinogen

vWf

conclusions
Conclusions
  • Adhesion over various proteins can be observed.
    • LbL Assembly
    • Fluorescent Staining/Microscopy
  • Adhesion is shear dependent.
  • Adhesion patterns depend on the protein substrate used.
future work
Future Work
  • Other substrates
  • Varieties of platelets
  • Quantification of adhesion patterns
  • In vivo testing
acknowledgements
Acknowledgements
  • Sachin Sah
  • Kanvasri Jonnalaggada
  • Ameya Kantak
  • Bruce Gale
  • David Mills
  • James Spaulding
  • Mike McShane
  • Hua Ai
  • IfM Staff
ad