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GLRI (#981) US Fish & Wildlife Service

GLRI (#981) US Fish & Wildlife Service. Environmental DNA Surveillance – Applied Early Detection. Christopher L. Jerde, Center for Aquatic Conservation, University of Notre Dame, Notre Dame, Indiana CO-PIs: Lodge, Mahon, & Chadderton. Introduction and Objectives.

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GLRI (#981) US Fish & Wildlife Service

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  1. GLRI (#981) US Fish & Wildlife Service Environmental DNA Surveillance – Applied Early Detection Christopher L. Jerde, Center for Aquatic Conservation, University of Notre Dame, Notre Dame, Indiana CO-PIs: Lodge, Mahon, & Chadderton

  2. Introduction and Objectives • eDNA is an indirect surveillance method • Correlation between proportion of positive detections and target species abundance • Currently used for presence/absence indicator • Objectives of the GLRI: • Evaluation of Asian carp presence in at least 14 Great Lakes rivers (Kolar et al. 2007) • Evaluation of Chicago area ponds for presence of invasive species, largely through organisms in trade • Development of 12 new eDNA molecular markers • Contribute to USFWS 2014 Great Lakes surveillance effort

  3. Current Knowledge • 425 GLRI samples collected Rivers Maumee (OH) St. Joseph (MI) Paw Paw (MI) Galien (MI) Galena (IN) Menomonee (WI) Milwaukee (WI) Kinnickinnic (WI) Chicago Ponds & Lagoons (IL)

  4. Current Knowledge (continued) • Bait trade sampling/results (IL-DNR) • 52 bait shops, 138 water samples • No positive AC detections • Bait trade calibration underway

  5. Future Plans • 2011 collections (~1500 samples) • Chicago area ponds (~250 samples) • Bait trade calibration studies completed

  6. Technological or Other Hurdles • Coordinating with state and federal partners to collect future samples • Getting USFWS up and running with eDNA processing

  7. Data Gaps • Calibration of large system detection patterns How many fish give rise to how many positive detections?

  8. GLRI: Indiana DNR Environmental DNA Surveillance in the Maumee-Eagle Marsh-Wabash Area Christopher L. Jerde, Center for Aquatic Conservation, University of Notre Dame, Notre Dame, Indiana, cjerde@nd.edu, 574-631-2665 CO-PIs: Mahon, Chadderton, & Lodge

  9. Introduction and Objectives • Unknown presence of bighead and silver carp in the Maumee-Eagle Marsh-Wabash intermittent hydrological connection • Upper most collection is at Roush Lake (bighead) • Objectives • Delimit the extent of Asian carp in the area • Provide recommendations for future sampling

  10. Current Knowledge • 247 samples collected across the hydrological connection including: • Wabash River (28) • Little Wabash River (60) • Graham-McCulloch Ditch (18) • Junk Ditch (14) • Eagle Marsh (31) • Aboit Creek (9) • Robinson Creek (6) • St. Mary River (21) • St. Joseph River (34) • Maumee River (17) • 9 Cooler controls

  11. Current Knowledge (continued) • No samples had detectable bighead or silver carp DNA • Caution: • Very low water levels • October sampling • High sediment load

  12. Future Plans • Final Report • Suggestions for spring sampling to IN-DNR • Spring sampling

  13. Technological or Other Hurdles • None

  14. Data Gaps • No defined edge of the Wabash invasion front

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