Additional file 1
This presentation is the property of its rightful owner.
Sponsored Links
1 / 1

Additional file 1 Evaluation of the number of integrated copies PowerPoint PPT Presentation


  • 57 Views
  • Uploaded on
  • Presentation posted in: General

Additional file 1 Evaluation of the number of integrated copies. A. 3.5 kb. AgeI. P-HTG. 2.0 kb. 1.6 kb. ATG. HTG. ∆neo. 1.4 kb. 0.9 kb. P SV40. P SV40. P SV40. P SV40. wt 1* 2* 3 4* 5. B. 21 kb. AgeI. 5.0 kb. 2.0 kb. 0.9 kb.

Download Presentation

Additional file 1 Evaluation of the number of integrated copies

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Additional file 1 evaluation of the number of integrated copies

Additional file 1

Evaluation of the number of integrated copies

A

3.5 kb

AgeI

P-HTG

2.0 kb

1.6 kb

ATG

HTG

∆neo

1.4 kb

0.9 kb

PSV40

PSV40

PSV40

PSV40

wt 1* 2* 3 4* 5

B

21 kb

AgeI

5.0 kb

2.0 kb

0.9 kb

- wt 1 2 3* 4 5* 6 7 8* 9 10 11 12

A: Analysis of bordering fragmentsfrom P-HTG tagged HEK293 cells.

lanes 1-5 individual clonesobtained upon tagging.

B: Analysis of bordering fragmentsof P-Ab-HTG antibodytagged CHO cells.

lanewt, untransfected CHO-K1 cells, lanes 1-12 correspondtoclones #1-12 in Fig. 2B.

Cloneswithsinglebandsaremarkedwith an asterisk.

wt: untransfectedcells; theneo probe usedforhybridizationisindicated.

Foranalysis, highmolecular DNA of individual clones was extractedaccordingto Ramirez-Solis et al., 1992. The DNA was cutwithAgeIandsubjectedto Southern Blotting. Fordetectionof bordering fragments, theblot was hybridizedtotheNeosequence. Each band correspondsto an individual integrationsite. Single bandsindicatesingleintegrationsites.

PAb-HTG

LC

HTG

∆neo

LC

Reference:

Ramirez-Solis, R., et al. (1992). Genomic DNA microextraction: a method to screen numerous samples. Anal. Biochem. 201: 331–335.


  • Login