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Luciana Baruffaldi, Maria Modanu, Maydianne CB Andrade

Luciana, looks great I’ve suggested a few additions not necessary but if helpful you can use them. In text boxes highlighted in yellow. Variation in sperm load unrelated to male morphology or developmental challenges in black widow spiders. Luciana Baruffaldi, Maria Modanu, Maydianne CB Andrade.

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Luciana Baruffaldi, Maria Modanu, Maydianne CB Andrade

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  1. Luciana, looks greatI’ve suggested a few additions not necessary but if helpful you can use them.In text boxes highlighted in yellow

  2. Variation in sperm load unrelated to male morphology or developmental challenges in black widow spiders Luciana Baruffaldi, Maria Modanu, Maydianne CB Andrade University of Toronto, Scarborough

  3. In mating systems in which females are polyandrous. Sperm from different males compete for fertilization. (Parker, et al, 1990) Argiope argentata Sperm competition drives evolution of morphology, behaviour, life history and ejaculate characteristics across taxa

  4. More ejaculated sperm leads to higher paternity. Sperm raffle – paternity split along same ratio as sperm numbers. Thalassoma bifasciatum (Warner et al, 1995)

  5. Male body condition. Evolution of ejaculate size. The risk and intensity of sperm competition. Large males have large ejaculates. Release more ejaculate in the presence of sneaker males. Gasterosteus aculeatus (Parker, 1990; Zbinden, et al, 2001,2004)

  6. Individual decisions about sperm allocation across females According to female promiscuity (releasing more ejaculate with virgin females) Females with large sexual ornaments (superior maternal investment) Invest less sperm in each mating but have more matings (Pizzari et al, 2003)

  7. Latrodectus hesperus Females mate more than once. Males show considerable variation in body size Is this scale bar shifted to the right? Thorax width

  8. Palps Palps: male’s paired intromittant reproductive organs.

  9. Sperm Induction No direct connection between gonads and palps Palps filled with sperm after final moult

  10. Considerable variation in sperm load in the palps Total sperm in one palp

  11. Objective • Analyze the variation in the number of sperm produced by male Latrodectushesperus spiders in relation to: • Male body size and • Experimental injuries

  12. Related to body size Evidence in other taxa. Large males = more sperm Large males = less sperm

  13. Related to injuries Adulthood reached through molting. Injuries can occur during molting. Developmental challenges that occur in nature during the male’s last molt How does damage to a sexual vs. non-sexual structure affect sperm load?

  14. Methods Leg Palp Day 1 amputation N=25 Day 7 N=25 Remove palps Count sperm

  15. Sperm counting

  16. Body size Length of tibia-patella Cephalothorax width

  17. CORRELATION BETWEEN RIGHT AND LEFT PALP

  18. SPERM IS NOT CORRELATED WITH MALE BODY SIZE

  19. P-amp L-amp SPERM IS INDEPENDENT OF THE TYPE OF INJURY

  20. Conclusion • Wide variation in sperm #: • unrelated to male morphology. SURPRISING BECAUSE IS EXTENDE IN ALL OF THE TAXA • Independent of the type of injury. SURPRISING BECAUSE IN NOT THE SAME LOSE A PALP THAN A LEG

  21. Future directions Diet during juvenile stages could affect sperm production? The size of the palp is related to storage of sperm? Or the condition of the male?

  22. Thanks!!!!!!!! Gracias!!!!!

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