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Supplementary Figure 1

Supplementary Figure 1. Supplementary Figure 2. Suppl. Fig. 1. The promoter of miR-155 encoding Bic gene contains two putative NF- k B binding sites.

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Supplementary Figure 1

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  1. Supplementary Figure 1

  2. Supplementary Figure 2

  3. Suppl. Fig. 1. The promoter of miR-155 encoding Bic gene contains two putative NF-kB binding sites. It is drown a partial nucleotide sequence of Bic promoter (from + 150 to – 222; from – 1064 to – 1912) containing the putative cis elements with higher score. The TATA box (-25 in pink), the AP1 site (-40 in red) the SP1 site (- 97 in blue), the two NF-kB binding sites (-1150; -1697 in green), and the PU.1 site ( -1403 in purple). Suppl Fig. 2. Inhibition of NF-kB canonical pathway leads to a reduction of miR-155 levels in EBV immortalized MC3 cells. A) Western Blot analysis of total protein extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-IkBa-S32/36A (MC3 IkBa) and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for IkBa expression. The exogenous protein runs higher than the endgenous because has HA-FLAG. B) Western Blot analysis of nuclear extracts obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-IkBa-S32/36A (MC3 IkBa) and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for p65 expression and normalized by H3. C) Graphic representation of the Western blot showed in B results as average of three independent experiments. The nuclear p65 decreases when the undegradable form of IkBa is expressed. D)Northern Blot analysis of total RNAs obtained from MC3 untransfected (MC3 UT), MC3 transfected with pRc/CMV-HA-IkBa-S32/36A (MC3 IkBa) and MC3 transfected with Empty Vector (MC3 EV). The blot was analyzed for miR-155 expression and normalized by U6. E) Graphic representation of the Northern blot results as average of three independent experiments.

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