slide1
Download
Skip this Video
Download Presentation
By: Hibah A. W. Abusulaiman

Loading in 2 Seconds...

play fullscreen
1 / 45

By: Hibah A. W. Abusulaiman - PowerPoint PPT Presentation


  • 111 Views
  • Uploaded on

Second year Lab Medicine (Females) 1433- 1434 A.H. / 2012- 2013 Microbiology Practical (Course I). By: Hibah A. W. Abusulaiman. Once upon a time…. Please don’t sleep. Fashionable friends. Abrar’s …. Sara started the preparation early. She got a luxurious manicure and padicure plus….

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' By: Hibah A. W. Abusulaiman' - kail


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
slide1

Second year Lab Medicine (Females)

1433- 1434 A.H. / 2012- 2013

Microbiology Practical (Course I)

  • By:

Hibah A. W. Abusulaiman

once upon a time

Once upon a time…

Please don’t sleep

after discussion with the doctor she knew it s because of the poor hygienic manicure and pedicure
After discussion with the Doctor, she knew it’s because of the poor hygienic manicure and pedicure
slide15
Good hygiene

Poor hygiene

simply because they are the agents of

Simply because they are the agents of…

Contamination, infection and decay

in the 1800s

In the 1800s

Aseptic techniques invented to prevent contamination of surgical wounds

many organizations classified infection control systems and substances into
Many organizations classified infection control systems and substances into:
  • Physical methods.
  • Chemical methods.

Referred broadly as Germicides

germicidal systems fall into three categories
Germicidal systems fall into three categories:
  • Decontamination.
  • Disinfection.
  • Sterilization.
1 decontamination
1. Decontamination:
  • Reduction of pathogenic microorganisms to be safe to handle without protective attire.
  • The Lowest level of control.
  • Includes physical cleaning with soap and detergents and removing of most organic and inorganic materials.
  • It is the critical step toward disinfection and steilisation.
2 disinfection
2. Disinfection:
  • Disinfectants are liquid chemical agents that kill targeted pathogens but not spores . It can also be solid or gaseous.
  • It is the next level of control.
3 sterilization
3. Sterilization
  • The complete elimination of viable organisms including spores.
  • The highest level of control
factors influencing disinfection
Factors influencing disinfection
  • Number of microorganisms.
  • Species and type of microorganism.
  • Temperature.
physical antimicrobial agents
Physical Antimicrobial Agents
  • Physical environmental agents can destroy large concentrations of microorganisms in lab cultures or a clinical specimen.
radiation
Radiation

UV Light

To inactivate microorganisms on surfaces

filtration
Filtration
  • Filtration is used to remove bacteria, fungi and their endospores from air or solutions.
osmotic pressure
Osmotic Pressure
  • Extremely hypertonic conditions can cause plasmolysis.
chemical antimicrobial agents
Chemical Antimicrobial Agents
  • In considering their application to patient care, two general classes:
    • Those destroying pathogenic microorganisms in the environment (disinfectants) or on skin (antiseptics).
    • Those administered to patients for treatment of infectious diseases (antimicrobial agents, often called antibiotics).
evaluating a disinfectant
Evaluating a disinfectant
  • Direct spray method.
  • Testing the quality of disinfectant from different brands.
slide39

Testing Disinfectant from Different Brands

0.5 mL

5 mLDettol

5 mL Clorox

5 mL DAC

5 mLGento

5 mL Bacterial Suspension in Nutrient Broth (Control)

slide40

Testing Disinfectant from Different Brands

Water Bath

Bacteria in Clorox

Bacteria in Dettol

Bacteria in DAC

Bacteria in Gento

Bacterial Suspension in Nutrient Broth (Control)

slide41

Testing Disinfectant from Different Brands

2 Loopfulls

48 hrs

Incubation

in the 37O C

Water Bath

After 5min

in Water Bath

After 10 min in

Water Bath

After 20 min in

Water Bath

Bacterial Suspension in Nutrient Broth (Control)

objectives
Objectives
  • Some infection control terms.
  • Factors influencing disinfectants.
  • Physical destruction of microorganisms.
  • Chemical destruction of microorganisms.
  • Methods of evaluating disinfectants.
ad