Body fluids
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Body Fluids. Prof. K. Sivapalan. Body Composition. Carbon, oxygen, hydrogen, nitrogen, ……. Muscles, bones, liver, spleen, brain…. Water 60 %. Protein 18-20 % Fat: M-15 %, F- 25 % Carbohydrate 2 % Minerals. Body Fat. Structural fat [6-7%] and storage fat Lean body mass

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Body Fluids

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Body fluids

Body Fluids

Prof. K. Sivapalan


Body composition

Body Composition

  • Carbon, oxygen, hydrogen, nitrogen, …….

  • Muscles, bones, liver, spleen, brain….

  • Water 60 %.

  • Protein 18-20 %

  • Fat: M-15 %, F- 25 %

  • Carbohydrate 2 %

  • Minerals.

Body Fluids


Body fat

Body Fat

  • Structural fat [6-7%] and storage fat

  • Lean body mass

  • Body mass index-

    • Weight in Kg/height in M2

Body Fluids


Fluid compartments

Fluid Compartments

  • Intra cellular fluid

  • Extra cellular fluid-Inter cellular fluid

    • Tissue fluid

    • Plasma

    • Transcellular fluids

Body Fluids


Body fluids

Body Fluids


Body fluids

Body Fluids


Major components

Major Components

Body Fluids


Body fluids

Body Fluids


Definitions solutions

Definitions - Solutions

  • A mole is the gram-molecular weight of a substance- NaCl- 23+35.5=58.5g

  • One electrical equivalent (eq) is 1 mol of an ionized substance divided by its valence.

  • Gram equivalent is the weight of a substance that is chemically equivalent to 8.000 g of oxygen

  • The normality (N) of a solution is the number of gram equivalents in 1 liter

  • Molar solution contains one gram mole of a substance in one liter.

Body Fluids


Body fluids

Body Fluids


Diffusion

Diffusion

  • Diffusion is the process by which a gas or a substance in a solution expands, because of the motion of its particles, to fill all the available volume.

  • The rate depends on ,

    • Concentration [chemical] gradient

    • Electrical gradient for charged particles

    • Cross-sectional area

    • Distance

    • Permeability of the boundaries

Body Fluids


Osmosis

Osmosis

  • Diffusion of solvent molecules into a region in which there is a higher concentration of a solute to which the membrane is impermeable—is called osmosis.

  • P=nRT/V [n is the number of particles, R is the gas constant, T is the absolute temperature, and V is the volume]

  • One osmole (Osm) equals the gram-molecular weight of a substance divided by the number of freely moving particles that each molecule liberates in solution

  • Osmolar solution- 1 osmole in 1 liter [op-22.4 atmosphears]

  • Glucose- mole=Osmole, NaCl- 1 mole = 2 osmoles, Na2so4- 1 mole = 3 osmoles.

Body Fluids


Osmolality tonisity

Osmolality - Tonisity

  • The osmolarity is the number of osmoles per liter of solution (eg, plasma)

  • The osmolality is the number of osmoles per kilogram of solvent.

  • Measured by the degree to which the freezing point is depressed [1.86 ºC]

  • Osmolality of plasma is 290 mOsm/L

  • The term tonicity is used to describe the osmolality of a solution relative to plasma- hypotonic, isotonic, hypertonic.

  • Osmotic Pressure is the pressure exerted by the solution when separated from water by semi-permiable membrane or the pressure required to prevent net movement of water into the solution.

  • Osmotic pressure of 1 osmolar solution is 22.4 atmospheres = 17024 mmHg, plasma= 17024x0.290=4936 mmHg.

Body Fluids


Importance of osmolality

Importance of Osmolality

  • Cells swell when exposed to extracellular hypotonicity and shrink when exposed to extracellular hypertonicity because cell membrane is freely permiable to water. [Fragility test for red cells]

  • If the concentration increases, reactions are altered and if the volume increases beyond the limit- lyses or contents diffuse out.

  • All but about 20 of the 290 mOsm in each liter of normal plasma are contributed by Na+ and its accompanying anions, principally Cl– and HCO3–.

  • Osmolality of plasma (mOsm/L) = 2[Na+] (mEq/L) + 0.055[Glucose] (mg/dL) + 0.36[Blood Urea Nitrogen] (mg/dL)

Body Fluids


Exchange between icf and ecf

Exchange Between ICF and ECF

  • The properties of the cell membrane determine the exchange.

  • Water is freely permeable

  • The permeability to solutes is determined by fat solubility and mollicular size.

  • Many substances are transported across the membrane by facilitated diffusion, active transport, secondary active transport, pinocytosis, phagocytosis etc.

Body Fluids


Transport across membrane

TRANSPORT ACROSS MEMBRANE

Body Fluids


Pinocytosis

PINOCYTOSIS

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Exchange between blood and tissues

Exchange Between Blood and Tissues

  • The exchange occurs through capillaries which lie between arteriol and venule.

  • Precapillarysphinctors determine the flow into specific capillaries which is determined by local metabolites.

  • The capillaries have clefts between endothelial cells [slit-pores] with diameter of 6-7 nm- smaller than albumin.

  • Fluid and solutes pass freely through these pores.

  • Plasmalemmal vesicles also transport small amounts.

Body Fluids


Diffusion in capillaries

Diffusion in Capillaries

  • Lipid soluble substances including oxygen and carbon dioxide diffuse across the endothelium according to concentration gradient.

  • Water and water soluble substances such as sodium, chloride, bicarbonate and glucose pass through the pores.

  • Larger molecules, albumin and other plasma proteins cannot pass through the pores

Body Fluids


Colloid osmotic pressure

Colloid Osmotic Pressure

  • The capillary pore permit all solutes in plasma except the cells and proteins.

  • It is similar to fluid separated by a semi-permiable membrane, the osmotic particle being the protein.

  • The osmotic pressure exerted by plasma proteins amount to about 25 mmHg.

  • Even though it is negligible contribution to the osmotic pressure of the plasma, it is the only difference across the capillary membrane.

  • It is named colloid osmotic pressure or oncotic pressure.

  • As the largest number of protein molecules are albumin, it is responsible for the colloid osmotic pressure

Body Fluids


Starling forces for capillary exchange

Starling Forces forCapillary Exchange

  • Pressure of the blood in the arteriolar end of the capillaries is 30 mm Hg.

  • It reduces progressively towards the venous end and becomes 10 mmHg.

  • Colloid osmotic pressure is 25 mmHg at the arteriolar end and becomes 28 mm Hg as concentration increases.

  • Tissue pressure is slightly negative [-3 mm Hg] probably due to lymphatic suction.

  • As some protein molecules escape into the tissue fluid, it also has mild colloid pressure [8 mm Hg].

Body Fluids


Starling equilibrium for capillary exchange

Starling Equilibrium forCapillary Exchange

  • At the arteriolar end:

    • Hydrostatic [blood] pressure= 30 mmHg

    • Plasma Colloid osmotic pressure= -25 mmHg

    • Tissue Colloid osmotic pressure= 8 mmHg

    • Tissue pressure= -3 mmHg

  • At the Venular end

    • Hydrostatic [blood] pressure= 10 mmHg

    • Plasma Colloid osmotic pressure= -28 mmHg

    • Tissue Colloid osmotic pressure= 8 mmHg

    • Tissue pressure= -3 mmHg

Body Fluids


Role of lymphatics

Role of Lymphatics

  • Allmost all tissues have lymphatics channels which coalase and open into superior venacava through thoracic duct [2-3 liters / day].

  • Exceptions include the superficial portions of the skin, the central nervous system, the endomysium of muscles, and the bones.

  • Lymphatic channels originate in the tissues as blind ended tube.

Body Fluids


Formation of lymph

Formation of Lymph

  • Lymphatic capillaries are made of endothelial cells which are attached to connective tissue through anchoring filaments.

  • The edge of one endothelial cell overlaps the adjacent cell so that the edge forms a minute valve that opens to the interior of the lymphatic capillary.

  • The lymphatics contract periodically and the valves prevent back flow and push the contents forwards.

  • This permits sucking of excess tissue fluid along with the protein and other large particles.

Body Fluids


Summary of microcirculation

Summary of Microcirculation

  • Filtration at the arteriolar end

  • Re-absorption at venular end

  • Balance fluid and proteins drained by lymphatics.

Body Fluids


Estimation of fluid volumes

Estimation of Fluid Volumes

  • The volume of a fluid compartment in the body can be measured by placing an indicator substance in the compartment,

  • allowing it to disperse evenly throughout the compartment’s fluid,

  • and then analyzing the extent to which the substance becomes diluted.

Body Fluids


Conditions for indicator dilution principle

Conditions for Indicator Dilution Principle

  • This method can be used to measure the volume of virtually any compartment in the body as long as :

  • (1) the indicator disperses evenly throughout the compartment,

  • (2) the indicator disperses only in the compartment that is being measured, and

  • (3) the indicator is not metabolized or excreted.

  • Several substances can be used to measure the volume of each of the different body fluids.

Body Fluids


Measurement of total body water

Measurement of Total Body Water

  • Radioactive water (tritium, 3H2O) or heavy water (deuterium, 2H2O) can be used to measure total body water.

Body Fluids


Measurement of extracellular fluid volume

Measurement of Extracellular Fluid Volume.

  • substances that disperse in the plasma and interstitial fluid but do not readily permeate the cell membrane are used.

  • They include radioactive sodium, radioactive chloride, radioactive iothalamate, thiosulfate ion, and inulin

Body Fluids


Measurement of plasma volume

Measurement of Plasma Volume

  • Substance used should not penetrate capillary membranes but remains in the vascular system after injection.

  • One of the most commonly used substances for measuring plasma volume is serum albumin labeled with radioactive iodine (125I-albumin).

  • Also, dyes that avidly bind to the plasma proteins, such as Evans blue dye (also called T-1824), can be used.

Body Fluids


Measurement of body fluid volumes

Measurement of Body Fluid Volumes

Body Fluids


Fluid balance ml day

Fluid Balance- ml/day

Body Fluids


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