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Foxp3

2.5. 16.5. 2.3. 5.3. 2.9. 2.4. 24. 71. 4.6. 23.4. 18. 9.4. 13.4. 13.9. 12. 23. 6. WT T con. WT T reg. +-. +IL2. +IL4. +IL7. 76. WT. Bim -/-. ***. 30. 100. % CD25 + Foxp3 + (in CD4 + cells). Count. 80. 20. Bim -/- T con. Bim -/- T reg. 60. Viability(%).

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Foxp3

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  1. 2.5 16.5 2.3 5.3 2.9 2.4 24 71 4.6 23.4 18 9.4 13.4 13.9 12 23 6 WT Tcon WT Treg +- +IL2 +IL4 +IL7 76 WT Bim-/- *** 30 100 % CD25+Foxp3+ (in CD4+ cells) Count 80 20 Bim-/- Tcon Bim-/-Treg 60 Viability(%) 10 40 20 BIM-/- Tcon BIM-/- Treg WT Tcon 0 WT Treg *** P = 0.0001 CFSE 0 WT Tcon WT Treg WT Tcon WT Treg WT Treg+IL7 8 22 10 Foxp3 PI Bim-/- Tcon Bim-/-Treg Bim-/- Tcon Bim-/-Treg Bim-/- Treg+IL7 53 FSC CFSE a b B6 CD45.1 Bcltg-CD45.1 BALB/C B6 Bim -/- Foxp3 Foxp3 CD4 CD4 B6 Bim -/- CD45.1 Bcltg-CD45.1 B6 BALB/C Foxp3 Foxp3 CD25 CD25 d e c g f

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