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Evolution Lab

Evolution Lab. Pre Lab Exercise:. 1. Name/Title/ Partners/ Date 2. Observations 3. Hypothesis- cladogram w/explanation 4. Data: Species/Max Score/ E value/ number of nucleotides in sequence First species for each gene Make data table.

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Evolution Lab

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  1. Evolution Lab

  2. Pre Lab Exercise: • 1. Name/Title/ Partners/ Date • 2. Observations • 3. Hypothesis- cladogram w/explanation • 4. Data: Species/Max Score/ E value/ number of nucleotides in sequence • First species for each gene • Make data table

  3. Results: In addition to the Analyzing Results questions on page S48 (1-4): • 1. How long is the sequence that was used to search the database? • Hint: This sequence is called the "query" sequence because you used it to ask a question (or query) of the database. • 2. What organism was the most likely source of the sequence? • Hint: Refer to the BLAST tutorial to find an overview of the GenBank nucleotide record. If more than one organism matches, look at the E values to determine the most likely match. • 3. What is the common name for this organism? • Hint: Refer to the GenBank nucleotide record. It may also help to look at the Taxonomy database.

  4. Conclusion: • Cladogramincluding fossil specimen. • Conclusion paragraph discussing whether or not your data/ conclusion supports your hypothesis.

  5. Lab Format: • 1. Title/Partners • 2. Objectives • 3. Background

  6. Lab Format: • 4. Procedure(s) • 5. Materials • 6. Data- reference some of the max scores and E values from your BLAST results

  7. Lab Format: • Results: Part Two • What is the function in humans of the protein produced from that gene? • Would you expect to find the same protein in other organisms? • Is it possible to find the same gene in two different kinds of organisms but not find the protein that is produced from that gene?

  8. Lab Format: • Results: Part Two (continued): • If you found the same gene in all organisms you test, what does this suggest about the evolution of this gene in the history of life on earth? • Does the use of DNA sequences in the study of evolutionary relationships mean that other characteristics are unimportant in such studies?

  9. Lab Format: • 8. Conclusion:

  10. Additional genes to research: • Fox P2 (Forkhead box P2)- language • Melanocortin receptor gene- skin color • Phi-hHaA gene- associated with hair- produces keratin in chimps and gorillas but not in humans • Microcephalin- brain size • ASPM- brain size

  11. More Genes: • Supersonic Hedgehog- embryonic body plan • Noggin- body organization – body axis • BMP-4- bottom genes • Hox- HomeoBox genes- body plan(segmentation)

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