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Course: medicinal plants

Course: medicinal plants. Muhammad Qasim Hayat. Alkaloids. group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms. also includes some related compounds with neutral  and even weakly acidic  properties Low molecular weight Nitrogenous compounds

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Course: medicinal plants

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  1. Course: medicinal plants Muhammad QasimHayat

  2. Alkaloids • group of naturally occurring chemical compounds that contain mostly basic nitrogen atoms. • also includes some related compounds with neutral and even weakly acidic properties • Low molecular weight Nitrogenous compounds • Mostly metabolic by-products derived from amino acids. • A.k.a. secondary metabolites • produced by a large variety of organisms, including bacteria, fungi, plants, and animals.

  3. Chemical structure • Alkaloids contain; • carbon,  • hydrogen  • nitrogen • In addition, may also contain  • oxygen,  • sulfur and • rarely other elements such as chlorine, bromine, and phosphorus. • contain one or more phenolic or indole rings, usually with a nitrogen atom in the ring • position of the nitrogen atom in the carbon ring varies with different alkaloids • it is the precise position of the nitrogen atom that effects the properties of these alkaloids.

  4. The boundary between alkaloids and other nitrogen-containing natural compounds is not clear-cut.  • Compounds like amino acid peptides, proteins, nucleotides, nucleic acid, amines, and antibiotics are usually not called alkaloids. • alkaloids are at times called a special case of amines.

  5. Chemical properties • Bitter and alkaline • Oxygen-containing alkaloids; • usually colorless crystals at ambient conditions. • Oxygen-free alkaloids; • are typically volatile, colorless, oily liquids. •  Some alkaloids are colored eg: sanguinarine (orange). • Alkaloids are poorly soluble in water but readily dissolve in organic solvents, such as diethyl ether, chloroform  • Exceptions such as  caffeine dissolves well in boiling water.[ • With acids, alkaloids form salts of various strengths. • Those salts are usually soluble in water and alcohol and poorly soluble in most organic solvents.

  6. Identification tests: Qualitative • Mayor’s test • 1 ml of extract + 1 ml of Mayer’s reagent (potassium mercuric iodide solution). Whitish or cream colored precipitate indicates the presence of alkaloids. • Wagner’s test • 1 ml of extract + 2 ml of Wagner’s reagent (iodine in potassium iodide). Reddish brown colored precipitate indicates the presence of alkaloids • Dragendorff’s test • 1 ml of extract + 1 ml of Dragendroff’s reagent (potassium bismuth iodide solution). An orange-red precipitate indicates the presence of alkaloids.  • Hager’s test • 1 ml of extract + 3 ml of Hager’s reagent (saturated aqueous solution of picric acid). Yellow colored precipitate indicates the presence of alkaloids

  7. Identification tests : Quantitative • Spectrophotometric determination • HPLC (high performance liquid chromatography)

  8. Extraction • Because of the structural diversity of alkaloids, there is no single method of their extraction from natural raw materials. • Most methods exploit the solubility of most alkaloids in organic solvents but not in water, and the opposite tendency of their salts. • Many alkaloids can be purified from crude extracts by acid-base extraction.

  9. Toxicology • Many alkaloids are toxic to other organisms. • Example • Pyrrolizidine alkaloids (PAs) are a large group of natural toxins produced by plants, several of which are known to be highly hepatotoxic and have been shown to be carcinogenic in rats. • They have been associated with a number of livestock diseases and with cases of human poisoning following consumption of herbal remedies or after contamination of staple foods. • Example plants in • Boraginaceae, Asteraceae, Orchidaceae families

  10. Medicinal properties • Alkaloid-containing plants have been used by humans since ancient times for therapeutic and recreational purposes. • For example, A Chinese book on houseplants written in 1st–3rd centuries BC mentioned a medical use of opium poppies. • They often have pharmacological effects and are used as medications • Some alkaloids have remarkable structural similarities with neurotransmitters in the central nervous system of humans, including dopamine, serotonin and acetylcholine. • The amazing effect of these alkaloids on humans has led to the development of powerful pain-killer medications.

  11. Applications

  12. Fats AND OILS Muhammad QasimHayat

  13. INTRODUCTION • Fats and Oils belong to a group of biological subtance called Lipids.lipids are biological chemicals do not dissolve in water FUNCTIONs • Regulatory messenger • Structural component of membrane • Energy store house How fats differ from oils? Fats differ from oils in that, they are solid at room temperature while oils are liquid they have same molecular structure

  14. Molecular structure of fats and oils. They are also called triglycerides.one of the reaction of triglycerides is is hydrolysis of ester groups.

  15. Structures of few important oils and fats. 1. 2. Docosahexaenoic acid 4. Castor oil 3. Olive Oil Triglycerides Triglyceride of linseed oil

  16. Medicinal properties of fats and oils Fats and oils have high medicinal value • They are high energy foods, providing about 9 calories per gram of fat.  • They are needed for your brain and nervous system, for energy production and for making most of the body’s vital hormones • two omega-3 essential fatty acids needed for development of the nervous system.  • coconut oil, along with coconut milk and coconut water, is highly praised today due to its high content oflauric acid, a natural infection fighter. • MCT or medium chain triglygceridesappear to assist energy production in some diseases such as Alzheimer’s disease omega-3 fatty acids which are also sometimes called essential fatty acids.  They are critical chemicals in our body that can help reduce inflammation, are needed for cell membranes, for nervous system development, and for many other functions in the body.

  17. Cholesterol is an essential fat compound manufactured in our livers that is needed to make all of the sex hormones and steroid hormones.  It is mainly made in our bodies. • Symptoms of omega-3 deficiency include rashes, dry skin, boils, joint pain, irritability, mental problems and much more. • Fats and oils coat the nerves with myelin, an important fatty substance that is needed to conduct nerve impulses properly. • Castor oil is cathartic it is also used as an emollient. • Shark liver oil is used in joint pain and with Vit-D used in burns and sun burns. • Linseed oil is used in scabies and skin diseases. • Olive oil is used as laxative.

  18. Chemical properties of fats • Fats and oils can be classified according to their degree of unsaturation as measured by their ability to absorb iodine at the double bonds. • Fats have relatively low iodine values and consist of glycerides containing high percentages of such saturated acids as lauric, myristic, and palmitic. • ; milk fats are usually characterized by the presence of short-chain carboxylic acids (butyric, caproic, and caprylic) • Fats and oils are hydrolized readily. This property is used extensively in the manufacture of soaps and in the preparation of fatty acids for industrial applications.

  19. Toxicity of fats and oils.  When an oil is saturated, that means that the molecule has all the hydrogen atoms it can hold. Unsaturation means that some hydrogen atoms have been removed, and this opens the structure of the molecule in a way that makes it susceptible to attack by free radicals.  Free radicals are reactive molecular fragments that occur even in healthy cells, and can damage the cell. When unsaturated oils are exposed to free radicals they can create chain reactions of free radicals that spread the damage in the cell, and contribute to the cell's aging.  Rancidity of oils occurs when they are exposed to oxygen, in the body just as in the bottle. Harmful free radicals are formed, and oxygen is used up.

  20. Identification tests for oil and fats used 1.Quality evaluation of oils and fats Transparency Color Odour Taste Relative density Refractive Index Smoke point Melting point Freezing point Determination of impurity Determination of acid value Determination of phospholipids Determination of soap Determination of saponification value .Determination of unsaponifiable matter .Determination of iodine value Determination of peroxide value Determination of carbonyl value

  21. 2. QUANTITATIVE CHEMICAL TESTS • Chemical Constants • Acid Values • Saponification Value • Ester Value • Iodine Value • Unsaponifiable Matter • Acetyl value • Physical Constants • Specific Gravity • Melting / Congealing Point • Refractive Index • Viscosity • Optical Rotation

  22. Extraction procedure Extraction by Expression Cold Expression Hot Expression Extraction by Solvents Hydrogenation sparging Extraction by expression crew presses are normally used to express oils from plant material because they give a better yield than older hydraulic presses. They also operate at higher pressure and continuously (not in batches).

  23. EXTRACTION BY EXPRESSION HOT EXPRESSION The residue left after cold expression is broken down and treated with steam. This causes the remaining oil cells to rupture. COLD EXPRESSION Oils for medicinal uses are extracted at room temperature. Only a portion of the oil is obtained.

  24. 2.EXTRACTION BY SOLVENT This type of method is used only for technical oils (not medicinal oils). Seeds used: Intact or partially extracted by expression. Solvent: Normally hexane (BP: 65 ºC)

  25. 3. Hydrogenation Oils may be partially hydrogenated to produce various ingredient oils. Lightly hydrogenated oils have very similar physical characteristics to regular soy oil, but are more resistant to becoming rancid. Margarine oils need to be mostly solid at 32 °C (90 °F) so that the margarine does not melt in warm rooms,  4. Sparging In the processing of edible oils, the oil is heated under vacuum to near the smoke point, and water is introduced at the bottom of the oil. The water immediately is converted to steam, which bubbles through the oil, carrying with it any chemicals which are water-soluble. The steam sparging removes impurities that can impart unwanted flavors and odors to the oil.

  26. EXAMPLE OF MEDICINAL PLANTS HAVING OILS AND FATS

  27. Glycosides Definition: Glycosides are a class of molecules in which, a sugar molecule (glycone) is bonded to a "non-sugar" (aglycone) molecule. Many plants store medicinally important chemicals in the form of inactive glycosides. The non-sugar portion contains the biochemically active properties of medical interest. Classification of glycosides according to a glycone part : 1-Anthracene ------- anthraquinone glycoside 2-Steroid ------------- steroidal glycoside (cardiac) 3-Triterpenoid –------ saponin glycoside

  28. Chemical structure

  29. Chemical properties 1.Separation between glycosides parts: Glycosides Hydrolysis glycone +aglycone +HCL Neutralization by +HCLdil Using alkaline Filtration (H2O+G)+A chloroform (H2O+G)+(chloroform+ A) We can separate them by using separatory funnel The best solvent to extract aglycone is Ethyl acetate because: i- immiscible in water. ii- always presents in the upper layer. 2. Glycosides also hydrolyzed by temperature or by using enzymes 3. They are water soluble compounds, insoluble in organic solvents. when a glycosides has a lot of sugars its solubility in water decrease. G + A +salt+H2O

  30. Toxicology • Therapeutic use of herbal cardiac glycosides continues to be a source of toxicity today. • Recently, D lanata was mistakenly substituted for plantain in herbal products marketed to cleanse the bowel; human toxicity resulted. • Cardiac glycosides have been also found in Asian herbal products and have been a source of human toxicity.

  31. Identification test(s) (qualitative + quantitative) A. Colorless, solid, amorphous, nonvolatile B. Give positive reaction (after hydrolysis) with Molisch's solution test Fehling's solution test

  32. Extraction procedure Always glycosides founded in the plant with the enzymes which hydrolyzed them. First damage these enzymes first to extract these glycoside by the following steps: 1-drying the plants fresh in special oven at 100 c for 30 minutes. 2-boiling them with organic solvents for 20 minutes 3- boiling them with acetone 5 minutes If present in this plant tannins or resins we add lead acetate to precipitate them.

  33. Examples (medicinal plants) 1-Salicin –salix- 2-Cascaroside _cascara 3-Aloin- Aloe vera 4- Sennoside – senna- 5-Frangulin – frangula 6- Glycyrrhizin – glycyrrhiza

  34. Known bioactivities • Local irritant group: • a-Sinigrin(Black mustered seeds_Brassicanigra) • b-Sinalbin(White mustered seeds_Brasica alba) • Analgesics and antipyretics: • SalicinSalisylic acid - Willow or Salix bark. • Keeping elasticity of blood vessels like: • Rutin_Rutoside (Bitter orange peels, Lemon peels) • Anti-inflammatory group: • a- Aloin for 1)acne 2)peptic ulcer • b-Glycyrrhizin

  35. Phyto-constituents of Medicinal Plants: Steroids Muhammad QasimHayat

  36. What are Steroids? • Steroids are artificial compounds, which have a similar composition to the natural male hormone testosterone. • Commonly referred to as anabolic(“to build” ) or androgenic (“masculinizing” )steroids.

  37. History of steroids Developed in the 1030’s to prevent the atrophy or break down of muscles in patients. Nazi doctors gave steroids to their soldiers in an attempt to make them more aggressive. The soviet union then decided to give steroids to their athletes. Once the U.S learned the soviet's secret, the also began giving steroids to their athletes, starting in the 1950’s

  38. How steroids work Anabolic steroids work by stimulating the anabolic effects by binding or plugging into protein receptors in or on the cells that help create new proteins in the cells. Anabolic steroids are taken orally, by injection, or with creams and patches

  39. How steroids work

  40. Chemical Structure • Steroids are a general class of agents that all have the steroid ring in common. • Comprised of three 6-carbon rings and one 5-carbon ring joined. • Cholesterol is the most basic form and, indeed, the precursor.

  41. Trans AB isomer Cis AB isomer • Shape very important for biological activity • 3D determined by ring junction AB – CD • AB-CD trans junction tend to have a flat, planar structure • important for hormonal activity • AB-CD cis junction are bent • allows them to fit on heart / smooth muscle and blood protein receptor sites • poisonous steroids – some used in heart disease

  42. Types of steroids Corticosteroids Testosterone

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