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Metabolic Biochemistry Lecture 9 August 19, 2005 Oxidative phosphorylation Other Pathways of Carbohydrate Metabolism Photosynthesis

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Metabolic Biochemistry Lecture 9 August 19, 2005 Oxidative phosphorylation Other Pathways of Carbohydrate Metabolism Photosynthesis. Import of NADH into mitochondria. Example of a shuttle for oxidizing NADH from the cytosol. LNC 19-27. LNC 19-26. Getting ATP and ADP

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slide1

Metabolic Biochemistry

Lecture 9

August 19, 2005

Oxidative phosphorylation

Other Pathways of

Carbohydrate Metabolism

Photosynthesis

slide2

Import of

NADH

into mitochondria

slide5

Getting ATP and ADP

in and out of

mitochondria

slide7

for NADH: P/O ratio ~ 2.5

for succinate: P/O ratio ~ 1.5

slide8

SUBSTRATE LEVEL PHOSPHORYLATION

GAP + Pi + NAD+ NADH + 1,3 bisphosphoglycerate

1,3 bisphosphoglycerate + ADP ATP + 3 phosphoglycerate

1 ATP produced

per NAD+ reduced

per GAP used up

per 3-PG produced

There is a precise stoichiometry

slide9

OXIDATIVE PHOSPHORYLATION

NADH + 1/2 O2 + H+ NAD+ + H2O

~ 3 ADP + ~ 3 Pi ~ 3ATP

<3 ATP produced per NADH oxidized

per H2O produced

per 1/2 O2 consumed

(P/O ratio ~ 2.5)

There is no precise stoichiometry,

because protons can find other paths

to return to the matrix

slide10

When succinate is the substrate, the P/O ratio is ~1.5,

because only two pumps are used (only 6 protons are pumped per succinate)

slide12

Mitochondria contain DNA (~16.5 kb)

mtDNA encodes

2 rRNAs

22 tRNAs

13 proteins (in animals)

mtDNA can have mutations causing

mitochondrial diseases

slide13

GLUCONEOGENESIS

LNC Chapter 14, Section 14.4

PENTOSE PHOSPHATE SHUNT

LNC Chapter 14, Section 14.5

slide15

phosphatase

third bypass

phosphatase

second bypass

fructose-1,6 bisphosphate

Phosphoenolpyruvate

PEP

first bypass

pyruvate

LNC Fig. 14-16

slide16

BIOTIN is a co-factor utilized for

CARBOXYLATION REACTIONS

THIAMINE PYROPHOSPHATE (TPP)

is a co-factor utilized for

DECARBOXYLATION REACTIONS

LNC Fig. 14-18

slide17

first bypass

in liver

in muscle

LNC Fig. 14-19

slide18

Chapter 15, Section 15.3 (pp.575)

Coordinated Regulation of Glycolysis and Gluconeogenesis

emphasis on regulation of

phosphofructokinase-1

and

fructose 1,6 bisphosphatase

slide20

Phosphofructokinase-1

4 identical subunits (two shown)

1 active site per subunit

1 site for allosteric regulator (ADP/ATP)

LNC Fig. 15.18

slide21

Phosphofructokinase-1

PFK-1

Fructose bis-phosphatase

FBPase

LNC Fig. 15-22

slide23

THE PENTOSE PHOSPHATE PATHWAY

Section 14.5

two significant products: ribose-phosphate

NADPH

slide24

End of Lecture 9

August 19, 2005

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