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Metabolic Biochemistry Lecture 9 August 19, 2005 Oxidative phosphorylation Other Pathways of Carbohydrate Metabolism Photosynthesis PowerPoint PPT Presentation


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Metabolic Biochemistry Lecture 9 August 19, 2005 Oxidative phosphorylation Other Pathways of Carbohydrate Metabolism Photosynthesis. Import of NADH into mitochondria. Example of a shuttle for oxidizing NADH from the cytosol. LNC 19-27. LNC 19-26. Getting ATP and ADP

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Metabolic Biochemistry Lecture 9 August 19, 2005 Oxidative phosphorylation Other Pathways of Carbohydrate Metabolism Photosynthesis

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Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Metabolic Biochemistry

Lecture 9

August 19, 2005

Oxidative phosphorylation

Other Pathways of

Carbohydrate Metabolism

Photosynthesis


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Import of

NADH

into mitochondria


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Example of a shuttle for oxidizing NADH from the cytosol

LNC 19-27


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

LNC 19-26


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Getting ATP and ADP

in and out of

mitochondria


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Biological work

LNC 19-25


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

for NADH: P/O ratio ~ 2.5

for succinate: P/O ratio ~ 1.5


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

SUBSTRATE LEVEL PHOSPHORYLATION

GAP + Pi + NAD+ NADH + 1,3 bisphosphoglycerate

1,3 bisphosphoglycerate + ADP ATP + 3 phosphoglycerate

1 ATP produced

per NAD+ reduced

per GAP used up

per 3-PG produced

There is a precise stoichiometry


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

OXIDATIVE PHOSPHORYLATION

NADH + 1/2 O2 + H+ NAD+ + H2O

~ 3 ADP + ~ 3 Pi~ 3ATP

<3 ATP produced per NADH oxidized

per H2O produced

per 1/2 O2 consumed

(P/O ratio ~ 2.5)

There is no precise stoichiometry,

because protons can find other paths

to return to the matrix


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

When succinate is the substrate, the P/O ratio is ~1.5,

because only two pumps are used (only 6 protons are pumped per succinate)


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Regulation of OXPHOS:


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Mitochondria contain DNA (~16.5 kb)

mtDNA encodes

2 rRNAs

22 tRNAs

13 proteins (in animals)

mtDNA can have mutations causing

mitochondrial diseases


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

GLUCONEOGENESIS

LNC Chapter 14, Section 14.4

PENTOSE PHOSPHATE SHUNT

LNC Chapter 14, Section 14.5


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

LNC Fig. 14-15


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

phosphatase

third bypass

phosphatase

second bypass

fructose-1,6 bisphosphate

Phosphoenolpyruvate

PEP

first bypass

pyruvate

LNC Fig. 14-16


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

BIOTIN is a co-factor utilized for

CARBOXYLATION REACTIONS

THIAMINE PYROPHOSPHATE (TPP)

is a co-factor utilized for

DECARBOXYLATION REACTIONS

LNC Fig. 14-18


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

first bypass

in liver

in muscle

LNC Fig. 14-19


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Chapter 15, Section 15.3 (pp.575)

Coordinated Regulation of Glycolysis and Gluconeogenesis

emphasis on regulation of

phosphofructokinase-1

and

fructose 1,6 bisphosphatase


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

LNC Fig. 14-16


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Phosphofructokinase-1

4 identical subunits (two shown)

1 active site per subunit

1 site for allosteric regulator (ADP/ATP)

LNC Fig. 15.18


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

Phosphofructokinase-1

PFK-1

Fructose bis-phosphatase

FBPase

LNC Fig. 15-22


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

LNC Fig. 15-23


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

THE PENTOSE PHOSPHATE PATHWAY

Section 14.5

two significant products:ribose-phosphate

NADPH


Metabolic biochemistry lecture 9 august 19 2005 oxidative phosphorylation other pathways of carbohydrate metabolism p

End of Lecture 9

August 19, 2005


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