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EASY TO USE MICROARRAY PRODUCTS-TODAY!. Pat Mayer Global Product Manager MICROMAX Feb. 2002. PerkinElmer Microarray Solution. Spotters. Data Analysis Software. GeneTAC G3. Scanners. Glass Slides. PerkinElmer Genomics. Slide Scanning Service. NEN Brand Nucleotide

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easy to use microarray products today
EASY TO USE MICROARRAY PRODUCTS-TODAY!

Pat Mayer

Global Product Manager

MICROMAX Feb. 2002

perkinelmer microarray solution
PerkinElmer Microarray Solution

Spotters

Data Analysis Software

GeneTAC G3

Scanners

Glass Slides

PerkinElmer

Genomics

Slide Scanning Service

NEN Brand Nucleotide

Analogs (Cyanine- and

Biotin-labeled)

GeneTAC Hybstation

Labeling Kits

Prespotted Microarrays

Complete Microarray Systems

why micromax products
Why MICROMAX Products?

MICROMAX

&

Differential Gene

Expression

why micromax products1
Why MICROMAX Products?

Differential gene expression

Gene expression

Relative levels of RNA and proteins

Differential gene expression

Differences in levels of RNA and proteins as a result of changes in cellular phenotype

backgound micromax technology1
Backgound MICROMAX Technology

Microarrays

  • A new and prominent technology that vies with PCR as the most significant commercial scientific advance of the decade.
  • Technology allows
    • Miniaturized format
    • Membrane or glass slide
    • Detection instrumentation
    • Analysis software
backgound micromax technology2
Backgound MICROMAX Technology

DNA Microarrays

  • Membrane Arrays
  • Oligonucleotide Arrays
  • cDNA or Long Oligo Arrays
backgound micromax technology3
Backgound MICROMAX Technology

Types of DNA Arrays

  • Membrane Arrays
    • cDNA spotted on nylon membrane
    • Rad nucleotides (chemiluminesence)
    • Gene expression and novel gene discovery
  • Advantages
    • Standard molecular biology techniques
    • Sensitive
    • Reusable
    • Unknown genes (ESTs) can be analyzed
  • Disadvantages
    • Single analysis per array
    • Low density
    • Radioactive safety
backgound micromax technology4
Backgound MICROMAX Technology

Types of DNA Arrays

  • Oligonucleotide chips
    • Oligo probes synthesized onto chip
    • Fluorescence detection
    • Gene expression and mutational analysis
  • Advantages
    • Very specific, can identify isotypic changes or SNPs
    • Tens of thousands oligos can be analyzed per chip
    • Automation
  • Disadvantages
    • Only known genes can be analyzed
    • Single analysis per array
    • Low sensitivity
    • Many spots make a gene
    • May be too specific for gene expression analysis
    • Not reusable
backgound micromax technology5
Backgound MICROMAX Technology

Types of DNA Arrays

  • Glass microarrays
    • cDNA or long oligos (50-70 mers) printed onto slides
    • Two-color fluorescence detection
    • Gene expression and novel gene discovery
  • Advantages
    • Dual detection for accurate differential analysis
    • Thousands of individual cDNA per slide
    • Unknown genes (ESTs) can be analyzed
    • Automation
  • Disadvantages
    • Low sensitivity
    • Not reusable
backgound micromax technology6

Reverse

Transcriptase

*P*

*P*

*P*

33P dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and detection for Microarrays

Direct - radioactivity

Preferred method for membrane arrays

** Single detection , Very sensitive**

backgound micromax technology7

Reverse

Transcriptase

F

F

F

fluoro- dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and Detection for Microarrays

Direct - fluorescence

Standard method for glass and oligo arrays

** Dual detection, Good sensitivity**

backgound micromax technology8

F

F

F

F

F

F

F

F

F

F

T

T

T

T

T

T

T

T

T

T

H

H

H

Y

Y

Y

Reverse

Transcriptase

B

B

B

biotin-dNTP

fluorescein-dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and Detection forMICROMAX

Indirect and amplified - Fluorescence

Excellent method for glass arrays (MICROMAX-TSA)

** Dual detection, Excellent sensitivity**

tsa technology review1
TSA Technology Review
  • TSA is a proprietary, PATENTED technology developed at NEN
    • Patented under the name CARD (Catalyzed Reporter Deposition)
    • Commercialized under the name TSA(Tyramide Signal Amplification)
tsa technology review2
TSA Technology Review

Keys To TSA

Inroduction of Horseradish Peroxidase (HRP) enzyme

Labeled Tyramide

tsa technology review3
TSA Technology Review

SIGNAL AMPLIFICATION

SIGNALAMPLIFICATION

SIGNAL AMPLIFICATION

tsa technology review4
TSA Technology Review

SENSITIVITY

SENSITIVITY

SENSITIVITY

tsa technology review5
TSA Technology Review

TSA

&

Microarray Technology

slide21

Signal Detection

Amplify

F

F

F

F

F

F

F

F

F

F

F

F

F

H

H

H

React with Tyramides

Y

Y

Y

T

T

T

T

T

T

T

T

T

T

T

T

T

Incubate with HRP- conjugate

Specific binding with

hapten containing probe

B

B

B

Sample immobilized to a surface - slide

  • Used existing patented and proprietary technology

TSA

Tyramide Signal Amplification

tsa on microarrays

Simultaneous hybridization

both Fluorescein and Biotin

labeled total RNA

Y

Y

Y

Y

Incubate with Anti-F-HRP

Amplify with TSA-cyanine 3

Inactivate ResidualHRP

Incubate with streptavidin-HRP

Amplify with TSA-cyanine 5

TSA on Microarrays

Sequential Fluorescence

Deposition with TSA

tsa technology review6
TSA Technology Review

Reduced Customer Sample Requirements

TSA reduces the input RNA sample up to 200-fold

Increases opportunity for microarray technology use

Tumor samples

Reduced RNA sample costs

Increased # of experiments

micromax microarray analysis with direct fluorescence and tyramide signal amplification
MICROMAX microarray Analysis with Direct Fluorescence and Tyramide Signal Amplification

4800 Slide

Direct Labeling Method

TSA Labeling Method

(100 µg Total RNA)

(1 µ g Total RNA)

TSA labeling method is very sensitive and needs as little as 0.5 to 1 µ gtotal RNA

direct and tsa labeling scatter plots
Direct and TSA Labeling: Scatter Plots

Direct

TSA

Jurkat: Cyanine 3

Jurkat: Cyanine 3

HL-60: Cyanine 5

HL-60: Cyanine 5

TSA labeling method is highly sensitive and identifies more differentially regulated genes

slide27

MICROMAX Platforms

Biotin/Fluorescein linked

dNTPs

Cyanine 3/5

dNTPs

OR

cDNA

labeling

isolate

total RNA

tissue samples

imaging

data analysis

hybridize

hybridize

TSATM amplified fluorescence detection

Fully integrated systems for streamlined application and consistent performance.

slide28

MICROMAX Products and Services =Flexibility

Microarray Products

Reagent Kits

Slide Scanning and Data

Processing Services

Cyanine 3- labeled dCTP, dUTP

TSA Kit

Direct Kit

Cyanine 5- labeled dCTP, dUTP

Complete

Microarray Systems

Quality Control microarray

(Practice Slide: 25 genes)

Human Screening microarrays

Human Focused microarrays

MICROMAXTM TSA

Microarray Systems

MICROMAXTM Direct

Microarray Systems

Glass Slides

ULTRACLEAN I

SUPERCHIP I

Human Screening Array System I&II

Human Array Systems I&II

Human Focused Array Systems

Chemical Labeling Kit

slide29

MICROARRAY SLIDES

  • 2 human cDNA
  • microarrays
  • 2 practice arrays
  • coverslips
  • cDNA PREPARATION
  • Cyanine 3-dUTP
  • Cyanine 5-dUTP
  • Reverse Transcriptase
  • (RT)/RNase inhibitor
  • dNTP labeling mix
  • Unlabeled control RNA
  • HYBRIDIZATION
  • Hybridization Buffer

INSTRUCTION MANUAL

System Components

MICROMAX Direct

slide30

cDNA PREPARATION

  • Biotin and Fluorescein
  • Nucleotides
  • dNTP Nucleotide Mix
  • Reverse Transcriptase
  • (RT)
  • RNase Inhibitor
  • Unlabeled control RNA
  • MICROARRAY SLIDES
  • 2 human cDNA
  • microarrays
  • 2 practice arrays
  • coverslips
  • cDNA QC CHECK AND HYBRIDIZATION
  • Biotin- and Fluorescein-Control cDNAs
  • GeneScreenTM Membrane
  • Streptavidin-HRP and Anti-Fluor HRP
  • Hybridization Buffer
  • DUAL FLUORESCENCE
  • DETECTION
  • TSA Amplification Buffer
  • Cyanine 3-Tyramide
  • Cyanine 5-Tyramide
  • HRP Inactivation Reagent

System Components

MICROMAX TSA

platform specifications
Platform Specifications

MICROMAX Direct

MICROMAX TSA

  • Sample Requirement
    • 20-80 ug Total RNA
  • Dynamic Range
    • 8-125,000 pg input mRNA (>104)
  • Differential Sensitivity
    •  2-fold ratio difference
  • Sample Requirement
    • 0.5-1 ug Total RNA
  • Dynamic Range
    • 0.04-200 pg input mRNA (>103.5)
  • Differential Sensitivity
    •  3-fold ratio difference
micromax products services
MICROMAX Products & Services

Complete MICROMAX Systems

MICROMAX Human cDNA Microarray System I-Direct

Direct Fluorescence Labeling System

5 slides

MICROMAX Human cDNA Microarray System 1.1-TSA

TSA Fluorescence Labeling System

5 slides

MICROMAX Human cDNA Microarray System II-TSA

TSA Fluorescence Labeling System

5 slides

Positioning

Everything for novice microarray user

micromax products services1
MICROMAX Products & Services

MICROMAXReagent Kits

MICROMAX Direct Reagent Kit

Direct Fluorescence Labeling Kit

10 Labeling Reactions

50 Labeling Reactions

MICROMAX TSA Labeling and Detection Kit

TSA Fluorescence Labeling Kit

10 Labeling & Amplifcation Reactions

40 Labeling & Amplifcation Reactions

Positioning

Researchers who manufacture their own microarrays

micromax products services2
MICROMAX Products & Services

MICROMAX Pre-SpottedMicroarrays

MICROMAX Microarray: Human cDNA 1.1 (2400 human genes)

MICROMAX Microarray: Human cDNA II (4800 human genes)

MICROMAX Microarray: Quality Control Slides

MICROMAX Microarray: Focused Array Slides (~270 human genes)

2 Microarrays

GeneMAP Microarrays: Human, Mouse and Rat

5 microarrays

Positioning

Experienced researchers who don’t have access to

cDNA arrays

Experienced researchers who have their own labeling

technology

micromax products services3
MICROMAX Products & Services

MICROMAX Slide Scanning Service

Scanning of all types of MICROMAX Slides

Practice Arrays free of charge

Slide Scanning Service Upgraded

Web Based Service

Positioning

Novice researchers who don’t have access to

fluorescent microarray scanner

selling genemap microarrays
Selling GeneMAP Microarrays
  • What are they?
    • Spotted, preprinted arrays
    • Human, mouse and rat glass microarrays
  • Available Worldwide (non-exclusive) from PerkinElmer
  • Sales of GeneMAP will count towards your MM sales goals!
    • Under a different pac code however, 2063
m micromax competition
MMICROMAX Competition

Who sells “Complete Systems”?

Labeling System

Nucleotides

Slides

ONLY PERKINELMER!

micromax competition1
MICROMAX Competition
  • Competition

Company Labeling Kits Slides

Affymetrix x X

Agilent X

Clontech X X

Amersham X

Stratagene X X

Genisphere X

Mergen X

HOMEBREW!!!! X X

micromax competition2
MICROMAX Competition

Competing Technologies

cDNA Labeling Technology

AminoAllyl

Clontech

Dendrimer

Genisphere

HOMEBREW

Slide Technology

Short (20-30 mers) Oligo

Affymetrix

Mergen

Medium (70-80 mers) Oligos

Clontech

cDNA

Agilent

Takara

HOMEBREW

micromax competition technologies
MICROMAX Competition-Technologies

Aminoallyl labeling technology…….

Amino groups are incorporated during cDNA synthesis using

aminoallyl-dUTP

Cy3 or Cy5 monomer NHS ester is reacted with cDNA

Labeling is independent of incorporation of dye-dNTP analogs

……is a novel technology which is cumbersome

TARGET CUSTOMER FOR NEW CHEMICAL LABELING!

micromax competition technologies1
MICROMAX Competition-Technologies

Genisphere’ s dendrimer technology…...

Labeling of cDNA RT with Unlabeled deoxynucleotides and Cy3™ or Cy5™ Primer Oligo (=3DNA Capture Sequence)

Hybridize to Array

Detect with 3DNA Fluor (Complementary Capture Sequence labeled with Cy-Dye™)

…...only competing technology to MICROMAX-TSA

Our customers have told us this doesn’t work very well-no specific signal

competitive advantages
Competitive Advantages
  • Low cost entry to microarray technology
  • Complete systems right out of the box
  • Broad product offering
  • License to do dual color fluorescence hybridization

Every lab can afford it!

Easy to use!

Something for every lab!

Two samples on one slide!

micromax selling
MICROMAX Selling

Probing Questions…...

Are you currently using microarray technology in your research?

Do you anticipate using microarray technology in the near future?

Is your differential gene expression research limited by the amount

of starting material?

Are you just getting started in microarray technology?

…….leads to providing customers with the right products

qc process for microarray products
QC Process for Microarray products
  • Labeling and Detection Kits
    • QC all components
    • Nucleotides HPLC QC
    • Nucleotides functional testing
    • Components used in functional testing of microarrays
    • Cross check in R & D and Applications Lab
  • Microarrays
    • Check PCR process
      • In process checks
    • Check autofluorescence of glass slides
    • Representative sequencing
    • Check after printing for spots
    • Functional testing of arrays
      • Spot morphology
      • Spot size
      • S/N
    • Cross check in R & D and Applications Lab
new micromax products in the last year
New MICROMAX products in the last year
  • 4800 Arrays and Systems (1.1) (Alphagene genes)
    • Now manufacture our own arrays
    • TSA and Direct versions
  • Direct Kit
    • more economical-$40/slide
  • Focused Arrays
    • Human Apoptosis & Neurobiology
  • Additional Screening Arrays and System (II)
    • Incyte Genomics’ Gene Content
  • Coming Soon
    • Glass Slides for DNA spotting
    • New Chemical Direct Labeling System
    • Mouse and Rat Screening Arrays
      • Content from LION bioscience
    • Control Kits

NEW

current micromax ads
Current MICROMAX Ads
  • MICROMAX TSA
    • “The NEN TSA Microarray Labeling and Detection Kit lets you do more with less”
    • Ran about 10 times in 2001-will be redesigned
  • MICROMAX Direct Kit Ad
    • “Simply the best microarray reagents on earth (or anywhere else you would care to explore!”
    • Ran about 2 times in 2001 and continue in 2002
  • MICROMAX Microarray Ad
    • “A long time ago. . .in a galaxy far away. . .we started making pre-spotted microarrays. . .We Still Do.”
    • Newly created and will run in 2002
  • MICROMAX Glass Slide Ad
    • “We have a new addition to the family. . .Introducing MICROMAX Glass Slides”
    • Newly created and will run in 2002
  • MICROMAX Chemical Direct Labeling Ad
    • Not created yet and will run in 2002
introducing micromax glass slides
Introducing MICROMAX Glass Slides
  • ULTRACLEAN I
    • Glass Slides that are cleaned!
    • For homebrew coating procedures
      • Looking for evaluators (e-mail Pat)
      • MPS692
  • SUPERCHIP I
    • Amino Coated Glass Slides
      • Looking for evaluators (e-mail Pat)
      • MPS695-5 slides
      • MPS696-20 slides

Ultra-flat

Low Intrinsic Fluorescence

Bundling opportunities with PerkinElmer printers and spotters!

Packaged in sets of 5 slides

Ready to ship evals February 11, 2002

Ready to ship by end of February

slide49

Breadth of Technical Support

  • In-field dedicated genomics specialists
  • Internal dedicated Technical Support
    • Leslie Stern X9117
  • Internal dedicated Applications Lab
    • Testing and expanding microarray protocols
  • Research and Product Development Staff
    • Organized for efficiency and time to market improvements
    • External seminar series
micromax sales tools available
MICROMAX Sales Tools Available

MICROMAX Seminar

Will be updated ASAP to incorporate Packard instruments

MICROMAX Literature Pieces

Code Name of Piece

H78255 MICROMAX Sell Sheet

H78511 MICROMAX TSA BioTechniques Reprint

P10208 MICROMAX Direct Reagent Kit Sell Sheet

H78414 MICROMAX TSA Sell Sheet

H78381 MICROMAX Focused Array Sell Sheet

H78416 MICROMAX TSA Protocol Laminated Card

Laminated card (H78416) is a must have for wet labs!

Order at www.perkinelmerliterature.com

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