Easy to use microarray products today
Download
1 / 50

EASY TO USE MICROARRAY PRODUCTS-TODAY! - PowerPoint PPT Presentation


  • 49 Views
  • Uploaded on

EASY TO USE MICROARRAY PRODUCTS-TODAY!. Pat Mayer Global Product Manager MICROMAX Feb. 2002. PerkinElmer Microarray Solution. Spotters. Data Analysis Software. GeneTAC G3. Scanners. Glass Slides. PerkinElmer Genomics. Slide Scanning Service. NEN Brand Nucleotide

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' EASY TO USE MICROARRAY PRODUCTS-TODAY!' - joshua


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Easy to use microarray products today

EASY TO USE MICROARRAY PRODUCTS-TODAY!

Pat Mayer

Global Product Manager

MICROMAX Feb. 2002


Perkinelmer microarray solution
PerkinElmer Microarray Solution

Spotters

Data Analysis Software

GeneTAC G3

Scanners

Glass Slides

PerkinElmer

Genomics

Slide Scanning Service

NEN Brand Nucleotide

Analogs (Cyanine- and

Biotin-labeled)

GeneTAC Hybstation

Labeling Kits

Prespotted Microarrays

Complete Microarray Systems



Why micromax products
Why MICROMAX Products?

MICROMAX

&

Differential Gene

Expression


Why micromax products1
Why MICROMAX Products?

Differential gene expression

Gene expression

Relative levels of RNA and proteins

Differential gene expression

Differences in levels of RNA and proteins as a result of changes in cellular phenotype



Backgound micromax technology1
Backgound MICROMAX Technology

Microarrays

  • A new and prominent technology that vies with PCR as the most significant commercial scientific advance of the decade.

  • Technology allows

    • Miniaturized format

    • Membrane or glass slide

    • Detection instrumentation

    • Analysis software


Backgound micromax technology2
Backgound MICROMAX Technology

DNA Microarrays

  • Membrane Arrays

  • Oligonucleotide Arrays

  • cDNA or Long Oligo Arrays


Backgound micromax technology3
Backgound MICROMAX Technology

Types of DNA Arrays

  • Membrane Arrays

    • cDNA spotted on nylon membrane

    • Rad nucleotides (chemiluminesence)

    • Gene expression and novel gene discovery

  • Advantages

    • Standard molecular biology techniques

    • Sensitive

    • Reusable

    • Unknown genes (ESTs) can be analyzed

  • Disadvantages

    • Single analysis per array

    • Low density

    • Radioactive safety


Backgound micromax technology4
Backgound MICROMAX Technology

Types of DNA Arrays

  • Oligonucleotide chips

    • Oligo probes synthesized onto chip

    • Fluorescence detection

    • Gene expression and mutational analysis

  • Advantages

    • Very specific, can identify isotypic changes or SNPs

    • Tens of thousands oligos can be analyzed per chip

    • Automation

  • Disadvantages

    • Only known genes can be analyzed

    • Single analysis per array

    • Low sensitivity

    • Many spots make a gene

    • May be too specific for gene expression analysis

    • Not reusable


Backgound micromax technology5
Backgound MICROMAX Technology

Types of DNA Arrays

  • Glass microarrays

    • cDNA or long oligos (50-70 mers) printed onto slides

    • Two-color fluorescence detection

    • Gene expression and novel gene discovery

  • Advantages

    • Dual detection for accurate differential analysis

    • Thousands of individual cDNA per slide

    • Unknown genes (ESTs) can be analyzed

    • Automation

  • Disadvantages

    • Low sensitivity

    • Not reusable


Backgound micromax technology6

Reverse

Transcriptase

*P*

*P*

*P*

33P dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and detection for Microarrays

Direct - radioactivity

Preferred method for membrane arrays

** Single detection , Very sensitive**


Backgound micromax technology7

Reverse

Transcriptase

F

F

F

fluoro- dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and Detection for Microarrays

Direct - fluorescence

Standard method for glass and oligo arrays

** Dual detection, Good sensitivity**


Backgound micromax technology8

F

F

F

F

F

F

F

F

F

F

T

T

T

T

T

T

T

T

T

T

H

H

H

Y

Y

Y

Reverse

Transcriptase

B

B

B

biotin-dNTP

fluorescein-dNTP

cDNA

mRNA

Backgound MICROMAX Technology

Labeling and Detection forMICROMAX

Indirect and amplified - Fluorescence

Excellent method for glass arrays (MICROMAX-TSA)

** Dual detection, Excellent sensitivity**



Tsa technology review1
TSA Technology Review

  • TSA is a proprietary, PATENTED technology developed at NEN

    • Patented under the name CARD (Catalyzed Reporter Deposition)

    • Commercialized under the name TSA(Tyramide Signal Amplification)


Tsa technology review2
TSA Technology Review

Keys To TSA

Inroduction of Horseradish Peroxidase (HRP) enzyme

Labeled Tyramide


Tsa technology review3
TSA Technology Review

SIGNAL AMPLIFICATION

SIGNALAMPLIFICATION

SIGNAL AMPLIFICATION


Tsa technology review4
TSA Technology Review

SENSITIVITY

SENSITIVITY

SENSITIVITY


Tsa technology review5
TSA Technology Review

TSA

&

Microarray Technology


Signal Detection

Amplify

F

F

F

F

F

F

F

F

F

F

F

F

F

H

H

H

React with Tyramides

Y

Y

Y

T

T

T

T

T

T

T

T

T

T

T

T

T

Incubate with HRP- conjugate

Specific binding with

hapten containing probe

B

B

B

Sample immobilized to a surface - slide

  • Used existing patented and proprietary technology

TSA

Tyramide Signal Amplification


Tsa on microarrays

Simultaneous hybridization

both Fluorescein and Biotin

labeled total RNA

Y

Y

Y

Y

Incubate with Anti-F-HRP

Amplify with TSA-cyanine 3

Inactivate ResidualHRP

Incubate with streptavidin-HRP

Amplify with TSA-cyanine 5

TSA on Microarrays

Sequential Fluorescence

Deposition with TSA


Tsa technology review6
TSA Technology Review

Reduced Customer Sample Requirements

TSA reduces the input RNA sample up to 200-fold

Increases opportunity for microarray technology use

Tumor samples

Reduced RNA sample costs

Increased # of experiments


Micromax microarray analysis with direct fluorescence and tyramide signal amplification
MICROMAX microarray Analysis with Direct Fluorescence and Tyramide Signal Amplification

4800 Slide

Direct Labeling Method

TSA Labeling Method

(100 µg Total RNA)

(1 µ g Total RNA)

TSA labeling method is very sensitive and needs as little as 0.5 to 1 µ gtotal RNA


Direct and tsa labeling scatter plots
Direct and TSA Labeling: Scatter Plots Tyramide Signal Amplification

Direct

TSA

Jurkat: Cyanine 3

Jurkat: Cyanine 3

HL-60: Cyanine 5

HL-60: Cyanine 5

TSA labeling method is highly sensitive and identifies more differentially regulated genes


MICROMAX Products & Services Tyramide Signal Amplification


MICROMAX Platforms Tyramide Signal Amplification

Biotin/Fluorescein linked

dNTPs

Cyanine 3/5

dNTPs

OR

cDNA

labeling

isolate

total RNA

tissue samples

imaging

data analysis

hybridize

hybridize

TSATM amplified fluorescence detection

Fully integrated systems for streamlined application and consistent performance.


MICROMAX Products and Services Tyramide Signal Amplification=Flexibility

Microarray Products

Reagent Kits

Slide Scanning and Data

Processing Services

Cyanine 3- labeled dCTP, dUTP

TSA Kit

Direct Kit

Cyanine 5- labeled dCTP, dUTP

Complete

Microarray Systems

Quality Control microarray

(Practice Slide: 25 genes)

Human Screening microarrays

Human Focused microarrays

MICROMAXTM TSA

Microarray Systems

MICROMAXTM Direct

Microarray Systems

Glass Slides

ULTRACLEAN I

SUPERCHIP I

Human Screening Array System I&II

Human Array Systems I&II

Human Focused Array Systems

Chemical Labeling Kit


  • MICROARRAY SLIDES Tyramide Signal Amplification

  • 2 human cDNA

  • microarrays

  • 2 practice arrays

  • coverslips

  • cDNA PREPARATION

  • Cyanine 3-dUTP

  • Cyanine 5-dUTP

  • Reverse Transcriptase

  • (RT)/RNase inhibitor

  • dNTP labeling mix

  • Unlabeled control RNA

  • HYBRIDIZATION

  • Hybridization Buffer

INSTRUCTION MANUAL

System Components

MICROMAX Direct


  • cDNA PREPARATION Tyramide Signal Amplification

  • Biotin and Fluorescein

  • Nucleotides

  • dNTP Nucleotide Mix

  • Reverse Transcriptase

  • (RT)

  • RNase Inhibitor

  • Unlabeled control RNA

  • MICROARRAY SLIDES

  • 2 human cDNA

  • microarrays

  • 2 practice arrays

  • coverslips

  • cDNA QC CHECK AND HYBRIDIZATION

  • Biotin- and Fluorescein-Control cDNAs

  • GeneScreenTM Membrane

  • Streptavidin-HRP and Anti-Fluor HRP

  • Hybridization Buffer

  • DUAL FLUORESCENCE

  • DETECTION

  • TSA Amplification Buffer

  • Cyanine 3-Tyramide

  • Cyanine 5-Tyramide

  • HRP Inactivation Reagent

System Components

MICROMAX TSA


Platform specifications
Platform Specifications Tyramide Signal Amplification

MICROMAX Direct

MICROMAX TSA

  • Sample Requirement

    • 20-80 ug Total RNA

  • Dynamic Range

    • 8-125,000 pg input mRNA (>104)

  • Differential Sensitivity

    •  2-fold ratio difference

  • Sample Requirement

    • 0.5-1 ug Total RNA

  • Dynamic Range

    • 0.04-200 pg input mRNA (>103.5)

  • Differential Sensitivity

    •  3-fold ratio difference


Micromax products services
MICROMAX Products & Services Tyramide Signal Amplification

Complete MICROMAX Systems

MICROMAX Human cDNA Microarray System I-Direct

Direct Fluorescence Labeling System

5 slides

MICROMAX Human cDNA Microarray System 1.1-TSA

TSA Fluorescence Labeling System

5 slides

MICROMAX Human cDNA Microarray System II-TSA

TSA Fluorescence Labeling System

5 slides

Positioning

Everything for novice microarray user


Micromax products services1
MICROMAX Products & Services Tyramide Signal Amplification

MICROMAXReagent Kits

MICROMAX Direct Reagent Kit

Direct Fluorescence Labeling Kit

10 Labeling Reactions

50 Labeling Reactions

MICROMAX TSA Labeling and Detection Kit

TSA Fluorescence Labeling Kit

10 Labeling & Amplifcation Reactions

40 Labeling & Amplifcation Reactions

Positioning

Researchers who manufacture their own microarrays


Micromax products services2
MICROMAX Products & Services Tyramide Signal Amplification

MICROMAX Pre-SpottedMicroarrays

MICROMAX Microarray: Human cDNA 1.1 (2400 human genes)

MICROMAX Microarray: Human cDNA II (4800 human genes)

MICROMAX Microarray: Quality Control Slides

MICROMAX Microarray: Focused Array Slides (~270 human genes)

2 Microarrays

GeneMAP Microarrays: Human, Mouse and Rat

5 microarrays

Positioning

Experienced researchers who don’t have access to

cDNA arrays

Experienced researchers who have their own labeling

technology


Micromax products services3
MICROMAX Products & Services Tyramide Signal Amplification

MICROMAX Slide Scanning Service

Scanning of all types of MICROMAX Slides

Practice Arrays free of charge

Slide Scanning Service Upgraded

Web Based Service

Positioning

Novice researchers who don’t have access to

fluorescent microarray scanner


Selling genemap microarrays
Selling GeneMAP Microarrays Tyramide Signal Amplification

  • What are they?

    • Spotted, preprinted arrays

    • Human, mouse and rat glass microarrays

  • Available Worldwide (non-exclusive) from PerkinElmer

  • Sales of GeneMAP will count towards your MM sales goals!

    • Under a different pac code however, 2063


Micromax competition
MICROMAX Competition Tyramide Signal Amplification

COMPETITION


M micromax competition
M Tyramide Signal AmplificationMICROMAX Competition

Who sells “Complete Systems”?

Labeling System

Nucleotides

Slides

ONLY PERKINELMER!


Micromax competition1
MICROMAX Competition Tyramide Signal Amplification

  • Competition

Company Labeling Kits Slides

Affymetrix x X

Agilent X

Clontech X X

Amersham X

Stratagene X X

Genisphere X

Mergen X

HOMEBREW!!!! X X


Micromax competition2
MICROMAX Competition Tyramide Signal Amplification

Competing Technologies

cDNA Labeling Technology

AminoAllyl

Clontech

Dendrimer

Genisphere

HOMEBREW

Slide Technology

Short (20-30 mers) Oligo

Affymetrix

Mergen

Medium (70-80 mers) Oligos

Clontech

cDNA

Agilent

Takara

HOMEBREW


Micromax competition technologies
MICROMAX Competition-Technologies Tyramide Signal Amplification

Aminoallyl labeling technology…….

Amino groups are incorporated during cDNA synthesis using

aminoallyl-dUTP

Cy3 or Cy5 monomer NHS ester is reacted with cDNA

Labeling is independent of incorporation of dye-dNTP analogs

……is a novel technology which is cumbersome

TARGET CUSTOMER FOR NEW CHEMICAL LABELING!


Micromax competition technologies1
MICROMAX Competition-Technologies Tyramide Signal Amplification

Genisphere’ s dendrimer technology…...

Labeling of cDNA RT with Unlabeled deoxynucleotides and Cy3™ or Cy5™ Primer Oligo (=3DNA Capture Sequence)

Hybridize to Array

Detect with 3DNA Fluor (Complementary Capture Sequence labeled with Cy-Dye™)

…...only competing technology to MICROMAX-TSA

Our customers have told us this doesn’t work very well-no specific signal


Competitive advantages
Competitive Advantages Tyramide Signal Amplification

  • Low cost entry to microarray technology

  • Complete systems right out of the box

  • Broad product offering

  • License to do dual color fluorescence hybridization

Every lab can afford it!

Easy to use!

Something for every lab!

Two samples on one slide!


Micromax selling
MICROMAX Selling Tyramide Signal Amplification

Probing Questions…...

Are you currently using microarray technology in your research?

Do you anticipate using microarray technology in the near future?

Is your differential gene expression research limited by the amount

of starting material?

Are you just getting started in microarray technology?

…….leads to providing customers with the right products


Qc process for microarray products
QC Process for Microarray products Tyramide Signal Amplification

  • Labeling and Detection Kits

    • QC all components

    • Nucleotides HPLC QC

    • Nucleotides functional testing

    • Components used in functional testing of microarrays

    • Cross check in R & D and Applications Lab

  • Microarrays

    • Check PCR process

      • In process checks

    • Check autofluorescence of glass slides

    • Representative sequencing

    • Check after printing for spots

    • Functional testing of arrays

      • Spot morphology

      • Spot size

      • S/N

    • Cross check in R & D and Applications Lab


New micromax products in the last year
New MICROMAX products in the last year Tyramide Signal Amplification

  • 4800 Arrays and Systems (1.1) (Alphagene genes)

    • Now manufacture our own arrays

    • TSA and Direct versions

  • Direct Kit

    • more economical-$40/slide

  • Focused Arrays

    • Human Apoptosis & Neurobiology

  • Additional Screening Arrays and System (II)

    • Incyte Genomics’ Gene Content

  • Coming Soon

    • Glass Slides for DNA spotting

    • New Chemical Direct Labeling System

    • Mouse and Rat Screening Arrays

      • Content from LION bioscience

    • Control Kits

NEW


Current micromax ads
Current MICROMAX Ads Tyramide Signal Amplification

  • MICROMAX TSA

    • “The NEN TSA Microarray Labeling and Detection Kit lets you do more with less”

    • Ran about 10 times in 2001-will be redesigned

  • MICROMAX Direct Kit Ad

    • “Simply the best microarray reagents on earth (or anywhere else you would care to explore!”

    • Ran about 2 times in 2001 and continue in 2002

  • MICROMAX Microarray Ad

    • “A long time ago. . .in a galaxy far away. . .we started making pre-spotted microarrays. . .We Still Do.”

    • Newly created and will run in 2002

  • MICROMAX Glass Slide Ad

    • “We have a new addition to the family. . .Introducing MICROMAX Glass Slides”

    • Newly created and will run in 2002

  • MICROMAX Chemical Direct Labeling Ad

    • Not created yet and will run in 2002


Introducing micromax glass slides
Introducing MICROMAX Glass Slides Tyramide Signal Amplification

  • ULTRACLEAN I

    • Glass Slides that are cleaned!

    • For homebrew coating procedures

      • Looking for evaluators (e-mail Pat)

      • MPS692

  • SUPERCHIP I

    • Amino Coated Glass Slides

      • Looking for evaluators (e-mail Pat)

      • MPS695-5 slides

      • MPS696-20 slides

Ultra-flat

Low Intrinsic Fluorescence

Bundling opportunities with PerkinElmer printers and spotters!

Packaged in sets of 5 slides

Ready to ship evals February 11, 2002

Ready to ship by end of February


Breadth of Technical Support Tyramide Signal Amplification

  • In-field dedicated genomics specialists

  • Internal dedicated Technical Support

    • Leslie Stern X9117

  • Internal dedicated Applications Lab

    • Testing and expanding microarray protocols

  • Research and Product Development Staff

    • Organized for efficiency and time to market improvements

    • External seminar series


Micromax sales tools available
MICROMAX Sales Tools Available Tyramide Signal Amplification

MICROMAX Seminar

Will be updated ASAP to incorporate Packard instruments

MICROMAX Literature Pieces

Code Name of Piece

H78255 MICROMAX Sell Sheet

H78511 MICROMAX TSA BioTechniques Reprint

P10208 MICROMAX Direct Reagent Kit Sell Sheet

H78414 MICROMAX TSA Sell Sheet

H78381 MICROMAX Focused Array Sell Sheet

H78416 MICROMAX TSA Protocol Laminated Card

Laminated card (H78416) is a must have for wet labs!

Order at www.perkinelmerliterature.com


ad