1 / 19

Anna-Louise Reysenbach reysenbacha@pdx, alrlab.pdx

Microbiological concepts explained using examples from deep-sea and terrestrial hydrothermal ecosystems. Anna-Louise Reysenbach reysenbacha@pdx.edu, www.alrlab.pdx.edu. Outline. Diversity of microbes Culturing the unculturables-use of 16S rRNA techniques Early evolution of life Astrobiology

jory
Download Presentation

Anna-Louise Reysenbach reysenbacha@pdx, alrlab.pdx

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Microbiological concepts explained using examples from deep-sea and terrestrial hydrothermal ecosystems Anna-Louise Reysenbach reysenbacha@pdx.edu, www.alrlab.pdx.edu

  2. Outline • Diversity of microbes • Culturing the unculturables-use of 16S rRNA techniques • Early evolution of life • Astrobiology • Other concepts/topics • Symbioses • Stress responses • Bioprospecting and biotech

  3. BACTERIA ARCHAEA EUKARYA

  4. Proteobacteria Bacteria • Anoxygenic phototrophs • H2S • S0 stored in cell e.g. Chromatium tepedium

  5. Green sulfur bacteria • Anoxygenic phototrophs • H2S • S0 outside the cell, SO42- • Generally tolerates > H2S than purples • Reverse citric cycle • photoheterotrophy Bacteria

  6. Cyanobacteria-oxygenic phototrophs Bacteria

  7. Cyanobacteria and Green non-S bacteria • Anoxygenic phototroph • “Mix between greenS and purple” • Can grow heterotrophically in dark by aerobic respiration • Unique CO2 fixation pathway, hydroxyproprionate pathway

  8. Ward et al., MMBR, 62:1353

  9. Ephydra thermophila

  10. Cyanidium & Dactylaria Eucarya Algae &Fungi pH 1 Lichen-like relationship? Nice eg.-Nymph Creek

  11. New Viruses from Yellowstone Amphitheater Springs 7/99 Rabbit Creek 10/00 Mud Volcano 8/00 G. Rice Rice et al., (2001) P.N.A.S.98/23 13341-5 K.M.S. 12/01

  12. Thermus worldwide distribution subsurface isolates heterotrophs iron reducers mixotrophic S-oxidizer PCR Bacteria

  13. Other thermophilic Bacteria • Plantomycetes • Gram +- Thermoterrabacterium • Spirochetes • Thermotoga • Hugenholtz et al.1998. J BAct Thermotoga Stetter et al

  14. A novel lineage within the Bacteria -sulfur reducers exclusive to deep-sea vents? Desulfurobacteriales-A new order for deep-sea vents? L’Haridon et al. 1998 Jeanthon and Reysenbach, (unpublished)

  15. Aquificales • “knall gas” • microaerophiles • or nitrate • Recently isolated from deep-sea vents Bacteria

  16. Sulfolobus • Crenarchaeote • Obligate aerobe • Some strains oxidize and reduce sulfur • Pyrite oxidizer • Chemolitho and hetero • WHY not at deep-sea vents???

  17. Biogeographicalconsiderations in the Euryarchaeota Thermococcus-cosmopolitan Methanothermus-endemic?

  18. The world record holders: High temperature--- Pyrolobus fumarii discuss mechanisms of thermal stability/ oxygen protection Smallest cells---- Nanoarchaeum (Nature) discuss method of isolation and treeing problems

  19. Korarchaeota • Slightly acidic, pH 6 • Yellowstone,Japan,reports from Kamchatka and New Zealand • No known physiology… • Grown as enrichments Archaea

More Related