Histology Techniques (CLS 322). Dr. Samah Kotb Nasr Eldeen. Topics were covered in theoretical part. Introduction To Histological Techniques. Different histological methods Fixation. Topics were covered in practical part. Lab safety. Different histological methods Fixation.
Dr. Samah Kotb Nasr Eldeen
Definition of histology:
Histology is the study of the tissues of the body and how these tissues are arranged to constitute organs.
Histotechnologist should not only know what to do; but should also know the principle and the theory of what he or she is doing.
These techniques are :
The specimens are accessioned by giving them a number that will identify each specimen for each patient.
Gross examination consists of describing the specimen and placing all or parts of it into a small plastic cassette which holds the tissue while it is being processed to a paraffin block. Initially, the cassettes are placed into a fixative.
The organic solvent must replace the water gradually to preventturbulence at the interface between water and pure ethanol
Impregnation in paraffin
paraffin was developed
Can be used in Histochemistry to demonstrate enzymes and chemical components of tissues.
Fixation is a process by which the internal constituents of tissue are preserved.
According to previous factors we can determine the
concentration of fixative and fixation time.