Research Training in Immunology at Brazilian Laboratory

1. Research Training in Immunology at Brazilian Laboratory Al Ali, E ; Alves, A.F.; Pacheco-Oliveira, M.; T. Muccini; Ramalho, T.R.; Costa, H.F.; Izidro-Bezerra, A.; Alves, A.K. A.; Paiva-Ferreira, L.K.D.; Vieira, G.C. and Piuvezam, M.R. enaamalali@yahoo.com / mrpiuvezam@ltf.ufpb.br Universidade Federal da Paraiba (UFPB) Programa de Pos-GraduacaoemProdutosNaturais e SinteticosBioativos (PgPNSB/UFPB) Introduction Hypersensitivity is an exaggerated response of the body against antigens. The process started by the exposure to antigen which lead to the activation of Th2 cells and B cells and IgE production that will bind to Fc receptor of mast cell. The reexpouser to the antigen will create a pathologic reaction. Inflammation is a body response against harmful agents, which is characterized by vasodilatation and fluids flow into the inflamed tissues. These events are mediated by various chemical agents for example PGE2, bradykinin and serotonin and leukocyte migration into the inflamed tissue. The cells migrate to the endothelium by attaching to selectin and integrin molecules on the endothelium cells. Finally, when the leukocytes reach the inflamed tissue they perform their functions and the inflammatory process may end or evolve to chronicity. Immunology is the science that study the recognition of aggressors and how the body defends itself from the action of these antigens. The Immunopharmacology Laboratory of the Federal University of Paraíba (UFPB) carries several lines of research, such as inflammation and hypersensitivity. Objective The purpose of this study was to evaluate the anti-inflammatory property of warifteine, an alkaloid of ​Cissampelos sympodialis, a medicinal plant used in Brazil to treat airway inflammatory processes. Materials and Methods Saline Saline Warifteine (2.0 mg/kg or 10.0 mg/gh)) 1 hour PGE2 carrageenan PGE2 , bradikinin or carrageenan bradikinin Results Paw edema Indomethacine Conclusion The results demonstrated that the compound Warifeine modulates the inflammatory process by decreasing the edema formation induced by phlogistic agents. These results will allow us to investigate more this plant and its compounds in order to prove that Cissampelos sympodialis can be used as an alternative medicine to treat inflammatory processes including allergic diseases. References Acknowledgements BARBOSA-FILHO, et al. Ciência e Cultura, 49,  386-94, 1997. PIUVEZAN et al. J. Ethnopharmacol, 67, 93-101, 1999 BEZERRA-SANTOS et al. Int. Immunopharmacol., 6 (7): 1152-60, 2006. COSTA et al. Int. Immunopharmacol, 8, 519-25, 2008. CERQUEIRA-LIMA et al. Current Drug Targets, 11, 2010 VIEIRA, G. C., 2012. Phytoterapy Research (accepted) ABBAS, et Al, (2007),Cellular and Molecular Immunology, 6th Edition, Saunders Elsever.