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Sterilization hot air oven Disinfection PowerPoint PPT Presentation


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Sterilization hot air oven Disinfection

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Sterilization hot air oven disinfection

(Antiseptic and Disinfectant)


Sterilization hot air oven disinfection

  • Sterilization () (hot air oven)

  • Disinfection ()

Disinfection

Sterilization

Antiseptic

Disinfectant


Sterilization hot air oven disinfection

(dry heat) (moist heat)

moist heat dry heat

ultraviolet 2540-2800 angstorm

Staphylococcus, Streptococcus virus

UV


Sterilization hot air oven disinfection

Antiseptics (/ )

  • ()

  • Disinfectant ( / )

  • ()


Antiseptic disinfectant

antiseptic disinfectant

  • 1.

  • 2.

  • 3.

  • 4.

  • 5.

  • 6. media /


Sterilization hot air oven disinfection

antiseptic disinfectant

1. detergent

2. gasoline


Sterilization hot air oven disinfection

Antiseptics Disinfectant

  • 1.

  • 2.

  • 3.

  • 4.

  • 5.


Sterilization hot air oven disinfection

Antiseptics Disinfectant ()

  • 6.

  • 7.

  • 8.

  • 9.


Sterilization hot air oven disinfection

disinfectant


Sterilization hot air oven disinfection

: Prince et al., 1991


Sterilization hot air oven disinfection

1. Alcohols

2. Aldehydes

3. Halogens

4. Heavy metals

5. Oxidizing agents

6. Phenols

7. Surface active agents

8. Acids

9. Nitrofurazone

10. Ethylene oxide

11. Miscellaneous


Sterilization hot air oven disinfection

: Prince et al., 1991


Alcohol

ALCOHOL

Alcohol 2 Ethanol Isopropanol


Ethanol

Ethanol

  • 60-95%

  • 70% () 1-2


Ethanol1

Ethanol ()

  • 5

  • 20% formalin 30

  • 0.2%

  • synergism disinfectant I, KI


Aldehyde

ALDEHYDE

Formaldehyde Glutaraldehyde

  • alkylation

  • acid fast


Formaldehyde

FORMALDEHYDE

  • Formalin (Formaldehyde 37%)

  • > 15 C


Glutaraldehyde

GLUTARALDEHYDE

  • Cidex (Glutaraldehyde 2% 70% Isopropanol)

  • formaldehyde


Halogens

HALOGENS

Halogens Iodine, chlorine

  • sulfhyhydryl (-SH) group S-S group


Iodines

IODINES

  • 2%

  • 1 : 20000 1 15


Iodophores

IODOPHORES

  • carrier

  • Povidone-Iodine (Betadine, Isodine)

  • Iodine + Polyvinylpyrolidone

  • 10%


Sterilization hot air oven disinfection

  • (HOCl)

  • 1-3 ppm


Sodium hypochlorite naocl

SODIUM HYPOCHLORITE (NaOCl)

  • 2-3% 2-3


Heavy metals

HEAVY METALS

  • sulfhydryl group


Sterilization hot air oven disinfection

  • ammonium mercury ointment

  • MerthiolateR(thimerosal) (Mercurochrome)


Sterilization hot air oven disinfection

  • silver nitrate

  • 0.1 % 1% 0.5%

  • methemoglobin


Oxidizing agents

OXIDIZING AGENTS

anaerobic facultative anaerobic microorganism


Hydrogen peroxide h 2 o 2

HYDROGEN PEROXIDE (H2O2)

  • 3% catalase

  • H2O2 free radical


Hydrogen peroxide h 2 o 21

HYDROGEN PEROXIDE (H2O2)

  • H2O2 oxidation 15-30 C


Potassium permanganate kmno 4

POTASSIUM PERMANGANATE (KMnO4)

  • oxidizing agent antiseptic

  • 1: 10000 1 .

  • 1: 5000


Phenols

PHENOLS

  • 1-2%

    • 1%

    • 2%

    • 5% ()


Cresols

CRESOLS

  • disinfectant

  • Phenol

  • isomer Phenol3

  • Lysol


Hexachlorophene

HEXACHLOROPHENE

CHLORHEXIDINE, CHLOROXYLENOL (Dettol)

  • xylenol Cl

  • Staphylococcus aureus


Sterilization hot air oven disinfection

DYES

  • Acriflavin () Gentian violet ()


Acriflavin

(Acriflavin)

  • Dermatophytes Candida esp. Staphylococcus

(Gentian violet)


Brilliant green

Brilliant Green

  • esp. Staphylococcus bacteriostatic E.coli

  • Brilliant Green + Gentian Violet


Surface active agents

SURFACE-ACTIVE AGENTS

detergent


Surface active agents1

SURFACE-ACTIVE AGENTS

Anionic

  • emulsifier

Cationic (Anionic)

  • Quaternary ammonium compound

  • ( Anionic) esp.

Emulsify =


Quaternary ammonium compound

Quaternary Ammonium compound

  • 0.1% Ethanol 70%


Acids

ACIDS

  • pH

  • disinfectant

  • pH

  • Acetic acid, Benzoic acid


Acetic acid

ACETIC ACID

  • 1%

  • 5%

  • 10-40%

SALICYLIC ACID


Boric acid

BORIC ACID

  • 3%

  • glycerine borax

  • Benzoic acid + Salicylic acid = Whitfield ointment

BENZOIC ACID


Sterilization hot air oven disinfection

Stage


Sterilization hot air oven disinfection

: AUSVETPLAN, 2000


Sterilization hot air oven disinfection

: Quinn, 1991


Sterilization hot air oven disinfection

esp.

  • 1


Sterilization hot air oven disinfection

  • bioaerosols

  • 20-30


Sterilization hot air oven disinfection

()

  • 1.

  • 2.

  • 2 -

  • 2 -


Sterilization hot air oven disinfection

()

  • 3.

  • 1


Sterilization hot air oven disinfection

bioaerosol

formaldehyde

. 2546. 9. . 95 .


Sterilization hot air oven disinfection

/

()

, 3, 4

/ 2, 3

3

1, 4.2

5.3

/

3

5.2

4

2, 4.2

3, 4

2, 2.3, 3, 4.2

1 = ; 2 = : 2.1 = sodium hpochloride, 2.2 calcium hypochloride; 3 = : 3.1 = sodium hydroxide, 3.2 = sodium carbonate; 4 = : 4.1 hdrochloric acid, 4.2 citric acid; 5 = aldehyde: 5.1 glutaraldehyde, 5.2 formalin, 5.3 formaldehyde gas


Sterilization hot air oven disinfection


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