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Sterilization hot air oven Disinfection PowerPoint PPT Presentation


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Sterilization hot air oven Disinfection

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(Antiseptic and Disinfectant)


  • Sterilization () (hot air oven)

  • Disinfection ()

Disinfection

Sterilization

Antiseptic

Disinfectant


(dry heat) (moist heat)

moist heat dry heat

ultraviolet 2540-2800 angstorm

Staphylococcus, Streptococcus virus

UV


Antiseptics (/ )

  • ()

  • Disinfectant ( / )

  • ()


antiseptic disinfectant

  • 1.

  • 2.

  • 3.

  • 4.

  • 5.

  • 6. media /


antiseptic disinfectant

1. detergent

2. gasoline


Antiseptics Disinfectant

  • 1.

  • 2.

  • 3.

  • 4.

  • 5.


Antiseptics Disinfectant ()

  • 6.

  • 7.

  • 8.

  • 9.


disinfectant


: Prince et al., 1991


1. Alcohols

2. Aldehydes

3. Halogens

4. Heavy metals

5. Oxidizing agents

6. Phenols

7. Surface active agents

8. Acids

9. Nitrofurazone

10. Ethylene oxide

11. Miscellaneous


: Prince et al., 1991


ALCOHOL

Alcohol 2 Ethanol Isopropanol


Ethanol

  • 60-95%

  • 70% () 1-2


Ethanol ()

  • 5

  • 20% formalin 30

  • 0.2%

  • synergism disinfectant I, KI


ALDEHYDE

Formaldehyde Glutaraldehyde

  • alkylation

  • acid fast


FORMALDEHYDE

  • Formalin (Formaldehyde 37%)

  • > 15 C


GLUTARALDEHYDE

  • Cidex (Glutaraldehyde 2% 70% Isopropanol)

  • formaldehyde


HALOGENS

Halogens Iodine, chlorine

  • sulfhyhydryl (-SH) group S-S group


IODINES

  • 2%

  • 1 : 20000 1 15


IODOPHORES

  • carrier

  • Povidone-Iodine (Betadine, Isodine)

  • Iodine + Polyvinylpyrolidone

  • 10%


  • (HOCl)

  • 1-3 ppm


SODIUM HYPOCHLORITE (NaOCl)

  • 2-3% 2-3


HEAVY METALS

  • sulfhydryl group


  • ammonium mercury ointment

  • MerthiolateR(thimerosal) (Mercurochrome)


  • silver nitrate

  • 0.1 % 1% 0.5%

  • methemoglobin


OXIDIZING AGENTS

anaerobic facultative anaerobic microorganism


HYDROGEN PEROXIDE (H2O2)

  • 3% catalase

  • H2O2 free radical


HYDROGEN PEROXIDE (H2O2)

  • H2O2 oxidation 15-30 C


POTASSIUM PERMANGANATE (KMnO4)

  • oxidizing agent antiseptic

  • 1: 10000 1 .

  • 1: 5000


PHENOLS

  • 1-2%

    • 1%

    • 2%

    • 5% ()


CRESOLS

  • disinfectant

  • Phenol

  • isomer Phenol3

  • Lysol


HEXACHLOROPHENE

CHLORHEXIDINE, CHLOROXYLENOL (Dettol)

  • xylenol Cl

  • Staphylococcus aureus


DYES

  • Acriflavin () Gentian violet ()


(Acriflavin)

  • Dermatophytes Candida esp. Staphylococcus

(Gentian violet)


Brilliant Green

  • esp. Staphylococcus bacteriostatic E.coli

  • Brilliant Green + Gentian Violet


SURFACE-ACTIVE AGENTS

detergent


SURFACE-ACTIVE AGENTS

Anionic

  • emulsifier

Cationic (Anionic)

  • Quaternary ammonium compound

  • ( Anionic) esp.

Emulsify =


Quaternary Ammonium compound

  • 0.1% Ethanol 70%


ACIDS

  • pH

  • disinfectant

  • pH

  • Acetic acid, Benzoic acid


ACETIC ACID

  • 1%

  • 5%

  • 10-40%

SALICYLIC ACID


BORIC ACID

  • 3%

  • glycerine borax

  • Benzoic acid + Salicylic acid = Whitfield ointment

BENZOIC ACID


Stage


: AUSVETPLAN, 2000


: Quinn, 1991


esp.

  • 1


  • bioaerosols

  • 20-30


()

  • 1.

  • 2.

  • 2 -

  • 2 -


()

  • 3.

  • 1


bioaerosol

formaldehyde

. 2546. 9. . 95 .


/

()

, 3, 4

/ 2, 3

3

1, 4.2

5.3

/

3

5.2

4

2, 4.2

3, 4

2, 2.3, 3, 4.2

1 = ; 2 = : 2.1 = sodium hpochloride, 2.2 calcium hypochloride; 3 = : 3.1 = sodium hydroxide, 3.2 = sodium carbonate; 4 = : 4.1 hdrochloric acid, 4.2 citric acid; 5 = aldehyde: 5.1 glutaraldehyde, 5.2 formalin, 5.3 formaldehyde gas



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