RNA Switches Genetic Research Tools RAVINDER NAGPAL 1 , MALVIKA MALIK 1 , MONICA PUNIYA 2 , AARTI BHARDWAJ 3 , SHALINI JAIN 4 and HARIOM YADAV 4* 1 Dairy Microbiology, 2 Dairy Cattle Nutrition, 4 Animal Biochemistry, National Dairy Research Institute, Karnal 132001,
RNA SwitchesGenetic Research Tools
RAVINDER NAGPAL1, MALVIKA MALIK1, MONICA PUNIYA2, AARTI BHARDWAJ3, SHALINI JAIN4 and HARIOM YADAV4*
1Dairy Microbiology, 2Dairy Cattle Nutrition, 4Animal Biochemistry,
National Dairy Research Institute, Karnal 132001,
Haryana, 3 Meerut Institute of Engineering and Technology, Meerut-250002, U.P., India.
DNA RNA PROTEIN
(Ellington and Szostak, 1990)
Regulation by Transcriptional Termination
Regulation by Translational Initiation Inhibition
Translation regulation by an mRNA Thermo sensor
This type of gene expression is given different names in different organisms.
Aptamers have been placed within 5' UTRs and shown to inhibit gene expression upon introduction of the appropriate ligand.(Werstuck and Green, 1998)
Self-cleaving RNAs can be placed under allosteric control by various small molecules, which can then be used to analyze the composition of chemical and biological mixtures .(Seetharaman et al, 2001)
RNA switches can be used in gene therapy, allowing the patient to take pills to switch introduced gene on or off.
An array of RNA molecular switches constructed using seven distinct effector-modulated ribozymes based on the hammerhead self-cleaving RNA.