ENZYME CLASSIFICATION EXERCISE
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ENZYME CLASSIFICATION EXERCISE. (1) GLUCOSE + ATP  GLUCOSE-6-PHOSPHATE + ADP + H + (2) CH 3 CH 2 OH + NAD +  (CH3)CHO + NADH + H + (3) ATP + H 2 O  ADP + P i (4) CH 3 (CO)COO - + H +  CH 3 CHO + CO 2 (PYRUVATE) (ACETALDEHYDE)

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ENZYME CLASSIFICATION EXERCISE

(1) GLUCOSE + ATP  GLUCOSE-6-PHOSPHATE + ADP + H+

(2) CH3CH2OH + NAD+ (CH3)CHO + NADH + H+

(3) ATP + H2O  ADP + Pi

(4) CH3(CO)COO- + H+  CH3CHO + CO2

(PYRUVATE) (ACETALDEHYDE)

(5) CH3(CO)COO- + ATP + CO2  ADP + Pi + -OOC-CH2 (C0) COO-

(OXALOACETATE)

-OOC H H H

| | | |

(6) C = C  C = C

| | | |

H COO- -OOC COO-


ENZYME CLASSIFICATION EXERCISE

(7) OXALOACETATE + GTP  PHOSPHOENOLPYRUVATE + GDP + CO2

(8) ASP + -KETOGLUTARATE  OXALOACETATE + GLU

(9) 3-PHOSPHOGLYCERATE  2-PHOSPHOGLYCERATE

(10) -OOC-CH2-CH2-COO- + FAD  -OOC-CH=CH-COO- + FADH2


ENZYME CLASSIFICATIONANSWERS TO IN-CLASS EXERCISE

  • (1) TRANSFERASE (HEXOKINASE)

  • (2) OXIDOREDUCTASE (ALCOHOL DEHYDROGENASE)

  • (3) HYDROLASE (ATPase)

  • (4) LYASE (PYRUVATE DECARBOXYLASE)

  • (5) LIGASE (PYRUVATE CARBOXYLASE)

  • (6) ISOMERASE (MALEATE ISOMERASE)

  • (7) HYDROLASE (PHOSPHOENOLPYRUVATE CARBOXYKINASE)

  • (8) TRANSFERASE (AMINOTRANSFERASE)

  • (9) PHOSPHOGLYCERATE MUTASE

  • (10) OXIDOREDUCTASE (SUCCINATE DEHYDROGENASE)


OXIDOREDUCTASES

  • CATALYZE REDOX REACTIONS

  • SOME COMMON TYPES:

    • REDUCTASES

    • DEHYDROGENASES

    • OXIDASES

    • OXYGENASES

  • LOOK FOR INVOLVEMENT OF COENZYMES

    • NAD+ , NADP+, FAD, FMN


TRANSFERASES

  • TRANSFER OF FUNCTIONAL GROUPS

  • SOME EXAMPLES:

    • TRANSFERASES

    • PHOSPHORYLASES

      • TRANSFER OF Pi OR PPi

    • KINASES

      • A PHOSPHATE TRANSFER

      • UTILIZE OR GENERATE ATP


HYDROLASES

  • HYDROLYSIS REACTIONS

  • WATER MOLECULES USED TO BREAK BONDS

  • EXAMPLES:

    • PHOSPHATASES

    • PEPTIDASES

    • ATPase, GTPase


LYASES

  • CATALYZES REACTIONS THAT

    • GENERATE A DOUBLE BOND

    • ADDS A SUBSTRATE MOLECULE TO DOUBLE BOND OF A SECOND SUBSTRATE

  • EXAMPLES:

    • DECARBOXYLASES

    • DEHYDRATASES

    • ALDOLASE


ISOMERASES

  • CONVERSION OF ONE ISOMERIC FORM INTO ANOTHER

  • EXAMPLES:

    • ISOMERASES

    • EPIMERASES

    • RACEMASES

    • MUTASES


LIGASES

  • TWO MOLECULES ARE JOINED

  • ANABOLIC REACTIONS

  • REQUIRE NUCLEOTIDES (ATP, GTP) TO DRIVE THEM

    • A PYROPHOSPHATE BOND MUST BE BROKEN

  • EXAMPLES:

    • CARBOXYLASE

    • SYNTHETASES

  • DON’T CONFUSE SYNTHETASES WITH SYNTHASES


Ongoing exercise
ONGOING EXERCISE

  • USING THE PRECEEDING PRINCIPLES OF ENZYME CLASSIFICATION, MAKE SURE THAT YOU CAN IDENTIFY THE CLASS OF ENZYME FOR EACH ENZYME THAT WE STUDY AND RATIONALIZE WHY IT FITS INTO THAT PARTICULAR CLASS


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