Vaginal discharge culture. Specimen processing for Vaginal swab. Vaginitis. Inflammation of the vaginal mucosa called vaginitis. Women who present with vaginal symptoms often complain of an abnormal discharge and possibly other symptoms such as offensive odor and itching.
Bacterial VaginosisAt least 10–20 high power (1000× oil immersion) fields are counted and an average determined.
Bacterial VaginosisGarm Stain
KOH slide A sample of the vaginal discharge is placed on a slide and mixed with a solution of 10% potassium hydroxide (KOH). The KOH kills bacteria and cells from the vagina, leaving only yeast for easier detection of a yeast infection.
Digest of casein.
Digest of animal tissue.
Dextrose. 4 %
Agar 1.5 %
Final pH 5.6 ± 0.2 at25°C
Sabouraud Dextrose agar is used for the isolation, cultivation, and maintenance of saprophytic and pathogenic fungi.
It supplies peptone as the protein source and dextrose as the carbohydrate source for nourishment .
Bacterial suppression occur due to the low pH (5.6) pH.
Note: chloramphenicol may use to inhibit growth of bacteria.
Candida appears as large, round, white or cream (albicans is from latin meaning 'whitish') colonies with a yeasty odor on agar plates.