CANDIDA & CRYTOCOCCUS. Prepared by: Miss Norzawani Jaffar Bsc ( Hons ) Biomedical Sciences, UKM. Lecture 15. Learning Outcomes. At the end of learning session, student must be able to ; Understand and describe the general morphology for Candida spp. and Cryptococcus spp.
CANDIDA & CRYTOCOCCUS
Bsc (Hons) Biomedical Sciences, UKM
At the end of learning session, student must be able to ;
Candida albicans, showing pseudohyphae with clamydospores, this fungus usually takes yeast
Candida albicans on blood agar; moist, opaque colonies are characteristic of yeast.
Candida albicans incubated in rabbit serum at 37° (germ tube test). Germ tubes are indicated by arrows and are the beginnings of true hyphae: no constriction is at the origin of the germ tube and the parent cell.
Candida tropicalis on a Dalmau plate.
Low power appearance of Candida parapsilosis on Dalmau plate. Note the characteristic "shaggy star" morphology.
Cryptococcus neoformans 1000x stained with Calcofluor.
Cryptococcus neoformans in blood culture; gram stain.
Cryptococcus laurentii on Dalmau plate. Note the absence of hyphae.
The colony colors indicate the following;
BLUE: C. albicans or C. dubliniensis (some strain of Trichosporon can be blue, but the colony morphology is very different from that of the Candida spp.)
PINK: C. tropicalis, C. guilliermondii, C. kefyr or C. lusitaniae (Cryptococcus spp., may also pink)
WHITE or CREAM: other yeast and some filamentaous moulds.
*Notes; Plates must always be stored and incubated in the dark. Some bacteria (notably Enterococcusfaecalis) may grow on the medium and have a pink or blue color.