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Introductory Questions #1. 1)What is the basic unit of measurement used by biologists to measure cells? What about internal organelles? 2) What are the approximate sizes for: -human egg cell-mitochondria -virus-protein

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Introductory questions 1

Introductory Questions #1

1)What is the basic unit of measurement used by biologists to measure cells? What about internal organelles?

2) What are the approximate sizes for:

-human egg cell-mitochondria

-virus-protein

3) What are the magnification limits of the human eye, a light microscope, and an electron microscope

4) How does a TEM differ from an SEM? What is the main limitation with using electron microscopes?

5) Briefly explain what the cell fractionation process does and how differential centrifugation can be helpful in the study of Cytology.

6) How do cells keep their internal contents separate from the outside environment?

7) Why is the surface to volume ratio an important factor with regard to cell size limits?


Introductory question 2

Introductory Question #2

1) Name three structures found in prokaryotic cells, eukaryotic plant cells, and eukaryotic animal cells.

2) Name the three layers that surround and protect a prokaryotic cell. Why are prokaryotes considered to be “simple” cells and eukaryotic are called “complex” cells?

Matching Ex.

Cellular respiration A. Nucleolus

Digests waste, worn out organellesB. Endoplasmic Ret.

Produces rRNA and ribosomes C. Ribosomes

Produces H2O2 D. Golgi Complex

Forms Mitotic spindle in Mitosis E. Lysosmes

Site for protein synthesis F. Peroxisomes

Site for the synthesis of lipidsG. Mitochondria

Modifies, packages and ships protein H. Centrioles


Introductory questions 1

IQ #3

What purpose do vesicles serve in the cell?

  • Name all of the organelles that are a part of the endomembrane system.

    4) Explain how the rough ER is different from the smooth ER,

    5) How is a lysosome different from a peroxisome?

    6) What do the chaperone proteins in the ER do?


Introductory questions 4

Introductory Questions # 4

  • Name the people that helped to develop the cell theory. What contribution did each person make (what did they discover)?


Introductory questions 1

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A tour of the cell

A Tour of the Cell

Chapter 6

(Pgs 94-123)

  • History & discoveries

  • Microscopy

  • Limits to Cell Size

    (Surface area to volume ratio)

  • Cell Fractionation

    (Structure & Function of Organelles)

  • Prokaryotic vs.Eukaryotic

  • Plant cells vs. Animal

  • Endomembrane System

  • Cytoskeleton

  • Intercellular junctions


History discovery of cells

History & Discovery of Cells

  • Anton Van Leeuwenhoek (pond water 1600’s)

  • Robert Hooke (Cork Cells, 1665)

  • Robert Brown (Nucleus, 1833)

  • Matthias Schleiden (Plant Cells, 1838)

  • Theodor Schwann (Animal Cells, 1839)

  • Rudolf Virchow(All Cells arise from other cells)

  • Cell Theory: 3 aspects


Introductory questions 1

  • Below is a list of the most common units of length biologists use (metric)

Table 4.2


Biological size and cell diversity pg 95

Biological Size and Cell Diversity (Pg. 95)

Human Eye: 1mm - meter+

LM: 1m – 1mm

EM: 1nm – 1mm

Chicken Egg (largest cell)

Mitochondria (1m)

Ribosomes (20-30 nm)

Viruses (80-100 nm)


Microscopes provide windows to the world of the cell

Microscopes provide windows to the world of the cell

  • The light microscope enables us to see the overall shape and structure of a cell

Image seen by viewer

Eyepiece

Ocularlens

Objective lens

Specimen

Condenser lens

Light source

Figure 4.1A


Introductory questions 1

  • Scanning electron microscope (SEM)

  • Scanning electron micrograph of cilia

Figure 4.1B


Introductory questions 1

  • Transmission electron microscope (TEM)

  • Transmission electron micrograph of cilia

Figure 4.1C


Cytology science study of cells

Cytology: science/study of cells

  • Light microscopy

  • resolving power~ measure of clarity

  • Electron microscopy (2 types)

    •TEM~ electron beam to study cell ultrastructure

    •SEM~ electron beam to study cell surfaces

  • Cell fractionation~ cell separation; organelle study

  • Ultracentrifuges~ cell fractionation; 130,000 rpm


Cell fractionation pg 97

Cell Fractionation-Pg 97


Cell fractionation

Cell Fractionation

  • Physically separates and purifies cell parts

  • Spun in a centrifuge (up to 500,000 rpm)

  • Two fractions: supernatant & pellet

  • Differential: successively at higher speeds

  • Density gradient: forms bands in tube according to density differences of organelles


Cell size

Cell Size

  • Is it more advantageous to be a single cell that is large or to be broken down into several small cells ?

    (Explain your answer)


Introductory questions 1

  • A small cell has a greater ratio of surface area to volume than a large cell of the same shape

30 µm

10 µm

Surface areaof one large cube= 5,400 µm2

Total surface areaof 27 small cubes= 16,200 µm2

Figure 4.3


Cell size surface area volume

Cell size - (surface area:volume)

  • As cell size increases, the surface area to volume ratio decreases (sa/vol)

  • Rates of chemical exchange may then be inadequate for cell size

  • Cell size, therefore, remains small


Natural laws limit cell size

Natural laws limit cell size

  • At minimum, a cell must be large enough to house the parts it needs to survive and reproduce

  • The maximum size of a cell is limited by the amount of surface needed to obtain nutrients from the environment and dispose of wastes


The prokaryotic cell see fig pg 98 also see pages 534 547 in ch 27

The Prokaryotic Cell-(See Fig. pg 98)(Also See Pages 534-547 in Ch. 27)

  • Characteristics include:

    • No true distinct nucleus

    • Have a “Nucleoid” region = DNA & Plasmids

    • No complex, membranous organelles (Ribosomes only)

    • Most have rigid cell walls

    • Flagella (rotary type structure & not composed w/microtubules)

    • Some have pigments (autotrophic)

    • Classified according to their metabolic needs

    • Eubacteria & Archeabacteria

    • Some have sticky capsules, pili, peptidoglycan, Endospores

    • Asexually Reproduce: Binary Fission, Budding, Fragmentation

    • Genetic Material Can be exchanged by 3 mechanisms:

      • Transformation, Transduction, and Conjugation


A prokaryotic cell

A Prokaryotic Cell


The eukaryotic cell

The Eukaryotic Cell

  • “Eu” = true“Karyo” = kernal (nucleus)

  • Protists, Plants, Fungi, and Animals

  • Internal Membrane System

  • Has many membranous organelles (Table 4.1) that include:

    -Nucleus-Lysosomes

    -Golgi complex-Endoplasmic reticulum (R & S)

    -Mitochondria-Chloroplast (plastids)

    -Peroxisomes (glyoxysomes)-Vesicles

    -Vacuole (food, contractile)-Ribosomes

  • Cytoskeleton: microtubules, microfilaments, and int. filaments

  • Centrioles (nine triplets of microtubules)

  • Cilia & Flagella (9+2 microtubule arrangement)

  • Extracellular matrix (ECM)-proteins & carbodydrate

    -glycoproteins-glycolipids-integrins

    -fibronectins-collagen


Introductory questions 1

Roughendoplasmicreticulum

Nucleus

Ribosomes

Smoothendoplasmicreticulum

Golgiapparatus

Microtubule

Centralvacuole

Not inanimalcells

Intermediatefilament

Cytoskeleton

Chloroplast

Microfilament

Cell wall

Mitochondrion

Peroxisome

Plasma membrane

Figure 4.5B


Introductory questions 1

Smooth endoplasmicreticulum

Nucleus

Roughendoplasmicreticulum

  • An animal cell

Flagellum

(exception is some plants)

Not in most plant cells

Lysosome

Centriole

Ribosomes

Peroxisome

Golgiapparatus

Microtubule

Plasma membrane

Cytoskeleton

Intermediatefilament

Microfilament

Mitochondrion

Figure 4.5A


Endomembrane function

Endomembrane Function

http://users.uma.maine.edu/SusanBaker/nucleus_endo.html


Nucleus ribosomes rough smooth er

Nucleus, Ribosomes, Rough & Smooth ER,

Flow of Genetic information and protein Synthesis


Nucleus pg 103

Nucleus (Pg. 103)

Control Center of the Cell

Genetic material:

•chromatin

•chromosomes

Nucleolus: ribosome synthesis

Double membrane envelope with pores

1st part of Protein synthesis:

Transcription (DNAmRNA)

Nuclear pores


Introductory questions 1

NUCLEUS

Chromatin

Two membranesof nuclearenvelope

Nucleolus

Pore

ROUGHENDOPLASMICRETICULUM

Ribosomes

Figure 4.6


Ribosomes

Ribosomes

  • Manufactures Protein

  • Free •cytosol; •protein function in cell

  • Bound •endoplasmic reticulum; •membranes, organelles, and export


Endoplasmic reticulum pg 105

Endoplasmic Reticulum (pg. 105)

Endoplasmic reticulum (ER)

  • Continuous with nuclear envelope

    Smooth ER

    •no ribosomes;

    •synthesis of lipids

    •metabolism of carbohydrates

    • detoxification of drugs and poisons

    Rough ER

    •with ribosomes

    •synthesis of secretory proteins (glycoproteins), membrane production

    **Found extensively in Pancreas


Rough endoplasmic reticulum makes membrane and proteins

Transport vesiclebuds off

4

Ribosome

Secretory(glyco-) proteininside transportvesicle

Sugarchain

3

Glycoprotein

1

2

ROUGH ER

Polypeptide

Rough Endoplasmic Reticulum makes membrane and proteins

  • The rough ER manufactures membranes

  • Ribosomes on its surface produce proteins

Figure 4.8


Introductory questions 1

SMOOTH ER

ROUGHER

Nuclearenvelope

Ribosomes

SMOOTH ER

ROUGH ER

Figure 4.9


Golgi complex pg 106

Golgi Complex (pg. 106)

  • Golgi apparatus

  • •ER products are modified, stored, and then shipped

  • Cisternae: flattened membranous sacs

  • trans face (shipping) & cis face (receiving)

  • Transport vesicles


The golgi apparatus finishes sorts and ships cell products

The Golgi apparatus finishes, sorts, and ships cell products

  • The Golgi apparatus consists of stacks of membranous sacs

    • These receive and modify ER products, then send them on to other organelles or to the cell membrane


Introductory questions 1

  • The Golgi apparatus

Golgi apparatus

Golgiapparatus

“Receiving” side ofGolgi apparatus

Transportvesiclefrom ER

Newvesicleforming

“Shipping”side of Golgiapparatus

Transport vesiclefrom the Golgi

Figure 4.10


Lysosomes digest the cell s food and wastes pg 107

Lysosomes digest the cell’s food and wastes (Pg.107)

  • Lysosomes are sacs of digestive enzymes budded off the Golgi

LYSOSOME

Nucleus

Figure 4.11A


Lysosomes

Lysosomes


Introductory questions 1

  • Contain lysosomal enzymes (hydrolytic enzymes)

  • digests food molecules (macromolecules)

  • destroys bacteria

  • recycles damaged organelles

  • function in embryonic development in animals

  • undergoes phagocytosis & engulfs material

  • Recycle cell’s own organic material

  • **Found extensively in Macrophages (WBC’s)

Lysosomes:


Introductory questions 1

Rough ER

Transport vesicle(containing inactivehydrolytic enzymes)

Plasmamembrane

Golgiapparatus

Engulfmentof particle

Lysosomeengulfingdamagedorganelle

“Food”

LYSOSOMES

Digestion

Foodvacuole

Figure 4.11B


Lysosomes can cause fatal diseases

Lysosomes can cause Fatal Diseases

  • Lysosomal Storage Diseases are hereditary that interfere with other cellular functions

    *Examples:

    Pompe’s disease (build up of glycogen)

    Tay-Sachs disease (lipid build up)

    (Pgs. 93, 331)


Vacuoles

Vacuoles

-Membrane-bound sacs (larger than vesicles)

-Food (phagocytosis)

-Contractile

(pump excess water)

-Central

(storage in plants)

-Tonoplast membrane


Vacuoles function in the general maintenance of the cell

Vacuoles function in the general maintenance of the cell

  • Plant cells contain a large central vacuole

    • The vacuole has lysosomal and storage functions

Centralvacuole

Nucleus

Figure 4.13A


Peroxisomes pg 111

Peroxisomes (Pg. 111)

  • Single membrane

  • Oxidative organelle

    ***strips e-’s (H’s) from substances

  • Produce hydrogen peroxide (H2O2) in cells

  • Metabolism of fatty acids; detoxification of alcohol (liver)

  • Hydrogen peroxide then converted to water


Mitochondria chloroplasts

Mitochondria & Chloroplasts

-Energy Harvesting Organelles


Mitochondria site of cellular respiration pg 110

Mitochondria -Site of Cellular Respiration(Pg. 110)


Mitochondria harvest chemical energy from food

Mitochondria harvest chemical Energy from food

  • Site for Cellular Respiration---Prod. of ATP

  • Uses O2 to extract energy from sugar, fats, and other molecules

  • Found in cells that are motile and contractible

  • Has a double membrane

  • Has Convoluted inner membranes: Cristae

  • Two spaces: Matrix & intermembrane space

  • Not part of the endomembrane system

  • Has its own DNA and rbosomes (able to regenerate & divide)---Semiautonomous


Introductory questions 1

MITOCHONDRION

Outermembrane

Intermembranespace

Innermembrane

Cristae

Matrix

Figure 4.16


Chloroplasts convert solar energy to chemical energy

Chloroplasts convert solar energy to chemical energy

  • Chloroplasts are found in plants and some protists

  • Chloroplasts convert solar energy to chemical energy in sugars

Chloroplast

Stroma

Inner and outer membranes

Granum

Intermembranespace

Figure 4.15


The chloroplast pg 111

The Chloroplast(pg. 111)

  • Site for Photosysnthesis: combines CO2 & H2O

  • Converts solar energy into chemical energy (sugar molecules)

  • A Type of Plastid

    • Three types: (Amyloplastid, chromoplast, and chloroplast)

  • Double membrane w/ thylakoids (flattened disks)

  • Grana (stacked thylakoids)

  • Three compartments

    -Stroma

    -Intermembrane space

    -Within the thylakoid membranes

  • Has its own DNA


The cytoskeleton pg 112 113

The Cytoskeleton (pg. 112-113)

-Fibrous proteins (actin & tubulin)

-Support, cell motility, biochemical regulation, organelle movement

-Microtubules: •thickest (nm) •tubulin protein; •shape, support, transport,

chromosome separation

-Microfilaments:

•thinnest ( nm)

•actin protein filaments;

•motility, cell division, shape

-Intermediate filaments:

• middle diameter; •keratin; •shape, nucleus anchorage


The cell s internal skeleton helps organize its structure and activities

The cell’s internal skeleton helps organize its structure and activities

  • A network of protein fibers makes up the cytoskeleton

Figure 4.17A


Comparing cytoskeletal filaments

Comparing Cytoskeletal Filaments

  • Scan image


The cytoskeleton

The Cytoskeleton

Tubulinsubunit

Actin subunit

Fibrous subunits

25 nm

7 nm

10 nm

MICROFILAMENT

INTERMEDIATEFILAMENT

MICROTUBULE

Figure 4.17B


Introductory questions 1

  • Intermediate filaments reinforce the cell and anchor certain organelles

  • Microtubules

    • give the cell rigidity

    • provide anchors for organelles

    • act as tracks for organelle movement

  • Microfilaments of actin enable cells to change shape and move


Cytoskeletal movement polymerization de polymerization

Cytoskeletal Movement(Polymerization & De-polymerization)


Centrosomes centrioles pg 114

Centrosomes/Centrioles(pg. 114)

  • Centrosome: region near nucleus

  • Centrioles: 9 sets of triplet microtubules in a ring;

    (used in cell replication; only in animal cells)


Cilia flagella pg 115 116

Cilia/Flagella (pg. 115-116)

-Locomotive appendages

-Ultrastructure: “9+2”

(9 doublets of microtubules in a ring)

(2 single microtubules in center)

-Connected by radial spoke

-Anchored by basal body

(nine triplets of microtubules)

-Dynein arm proteins (red)


Cilia and flagella move when microtubules bend

Cilia and flagella move when microtubules bend

  • Eukaryotic cilia and flagella are locomotor appendages that protrude from certain cells

  • A cilia or flagellum is composed of a core of microtubules wrapped in an extension of the plasma membrane


Introductory questions 1

FLAGELLUM

Electron micrograph of sections:

Outer microtubule doublet

Plasmamembrane

Flagellum

Centralmicrotubules

Outer microtubule doublet

Plasmamembrane

Basal body

Basal body(structurally identical to centriole)

Figure 4.18A


Dynein arm function pg 116

Dynein Arm Function(pg. 116)


Introductory questions 1

  • Clusters of microtubules drive the whipping action of these organelles

Microtubule doublet

Slidingforce

Dynein arm

Figure 4.18B


Ecm composition

ECM Composition

  • Extracellular matrix (ECM) composed of:

    -Proteins & Carbodydrate

    -Specifically:

    -glycoproteins

    -glycolipids

    -integrins

    -fibronectins

    -collagen (50% of all protein in the body)


Extracellular matrix ecm pg 118 120

Extracellular Matrix (ECM) - Pg. 118-120

Glycoproteins: •proteins covalently bonded to carbohydrate

Collagen

(50% of protein in human body

•embedded in proteoglycan

(another glycoprotein-95% carbohydrate)

Fibronectins

bind to receptor proteins in plasma

membrane called integrins

(cell communication?)


Introductory questions 1

  • Animal cells are embedded in an extracellular matrix

  • It is a sticky layer of glycoproteins

  • It binds cells together in tissues

  • It can also have protective and supportive functions


Intracellular junctions pg 121

Intracellular Junctions(pg. 121)

  • PLANTS:

  • Plasmodesmata:

    cell wall perforations; water and solute passage in plants

  • ANIMALS:

  • Tight junctions~ fusion of neighboring cells; prevents leakage between cells

  • Desmosomes~ riveted, anchoring junction; strong sheets of cells

  • Gap junctions~ cytoplasmic channels; allows passage of materials or current between cells


Cell surfaces junctions

Cell surfaces & Junctions

-Cell wall: •not in animal cells

•protection, shape, regulation

-Plant cell: •primary cell wall produced first

•middle lamella of pectin (polysaccharide)

-Holds cells together

•some plants have a secondary cell wall; strong durable matrix; wood

(between plasma membrane and primary wall)


Introductory questions 1

Walls of two adjacent plant cells

Vacuole

PLASMODESMATA

Layers of one plant cell wall

Cytoplasm

Plasma membrane

Figure 4.19A


Introductory questions 1

  • Tight junctions can bind cells together into leakproof sheets

  • Anchoring junctions link animal cells

  • Communicating junctions allow substances to flow from cell to cell

TIGHTJUNCTION

ANCHORING JUNCTION

COMMUNICATING

JUNCTION

Plasma membranes ofadjacent cells

Extracellularmatrix

Figure 4.19B


Movin on to chapter 7

Movin’ on to Chapter 7


Science and art

Science and Art


The art of looking at cells

The Art of Looking at Cells

  • Artists are often inspired by biology and biology depends on art

  • The paintings of Wassily Kandinsky (1866-1944) show the influence of cellular forms


Introductory questions 1

  • Illustration is an important way to represent what scientists see through microscopes

  • The anatomist Santiago Ramón y Cajal (1852-1934) was trained as an artist

    • He drew these retina nerve cells


Eukaryotic organelles comprise four functional categories

Eukaryotic organelles comprise FOUR functional categories

Table 4.20


Summary of organelles their function

Summary of Organelles & their Function

Table 4.20 (continued)


A review of the endomembrane system

A review of the endomembrane system

  • The various organelles of the endomembrane system are interconnected structurally and functionally

Transport vesiclefrom Golgi

Transport vesiclefrom ER

Rough ER

Plasmamembrane

Vacuole

Nucleus

Lysosome

Golgiapparatus

Smooth ER

Nuclearenvelope

Figure 4.14


Extraterrestrial life forms may share features with life on earth

Extraterrestrial life-forms may share features with life on Earth

  • It is almost certain that Earth is the only life-bearing planet in our solar system

  • But it is conceivable that conditions on some of the moons of the outer planets or on planets in other solar systems have allowed the evolution of life

Figure 4.21


Samples of various types of cells

Samples of Various Types of Cells


Introductory questions 1

Nucleus

Contractilevacuoles

  • These pump out excess water

  • Protists may have contractile vacuoles

Figure 4.13B


Introductory questions 1

  • Cell, stained for mitochondria, actin, and nucleus

Figure 4.1x


Introductory questions 1

  • Prokaryotic cells, Bacillus polymyxa

Figure 4.4x1


Introductory questions 1

  • Prokaryotic cell, E. coli

Figure 4.4x2


Introductory questions 1

  • Pili on a prokaryotic cell

Figure 4.4x3


Introductory questions 1

  • Prokaryotic flagella

Figure 4.4x4


Introductory questions 1

  • Prokaryotic and eukaryotic cells compared

Figure 4.4x5


Introductory questions 1

  • Paramecium, an animal cell

Figure 4.5Ax


Introductory questions 1

  • Plant cells

Figure 4.5Bx1


Introductory questions 1

  • Chloroplasts in plant cells

Figure 4.5Bx2


Introductory questions 1

  • Nuclei (yellow) and actin (red)

Figure 4.6x


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