Structure and composition of nuclear pore complexes npcs
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Structure and composition of nuclear pore complexes (NPCs). Electron micrographs of NPCs. Gall, 1967. Electron micrographs of NPCs. Gall, 1967. Three-dimensional view of a nuclear pore in the double-layered nuclear envelope. Gall, 1967.

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Structure and composition of nuclear pore complexes (NPCs)

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Structure and composition of nuclear pore complexes (NPCs)


Electron micrographs of NPCs

Gall, 1967


Electron micrographs of NPCs

Gall, 1967


Three-dimensional view of a nuclear pore in the double-layered nuclear envelope

Gall, 1967


Diagram of a model cylinder with the x-, y- and z-axes

Akey & Radermacher, 1993


Averaged xy slices taken from the 3D map of the NPC

Akey & Radermacher, 1993


3D side view of the NPC surface map

Akey & Radermacher, 1993


z slices taken from 3D maps of the NPCs

Akey & Radermacher, 1993


Surface views of the NPC 3D map

Akey & Radermacher, 1993


3D representation of the membrane-associated NPC

Akey & Radermacher, 1993


3D structure of the NPC

Beck et al, 2004


Cutaway view of the NPC

Beck et al, 2004


Slices through the structure of the pore

Beck et al, 2004


The NPC nuclear basket

Kiseleva et al, 2004


The NPC cytoplasmic particles

Kiseleva et al, 2004


The NPC cytoplasmic particles

Kiseleva et al, 2004


Immuno-labelling of Nup116

Kiseleva et al, 2004


Summary

The NPC has eight rotational symmetry.

The NPC is constructed from three main parts: 1) cytoplasmic components 2) central parts3) nucleoplasmic components

The NPC is huge structure. The vertebrate NPC has about 125 nm diameter (around 20 times larger than a ribosome).


Comparison of the peripheral and intrinsic membrane polypeptides of nuclear envelopes and microsomal membranes

Gerace et al, 1982


Characterization of antibodies to gp 190 and the lamins

Gerace et al, 1982


Immunofluorescence staining of tissue culture cells with anti-gp 190 and anti-lamins A and C

Gerace et al, 1982


Immuno-labeling of nuclei with anti-gp 190

Gerace et al, 1982


Presence of gp 190 in diverse vertebrate species

Gerace et al, 1982


Mammalian, yeast and worm nucleoporins

Angelo & Hetzer, 2008 (Review)


Summary

The NPC is composed of ONLY about 30 different proteins (nucleoporins). Ribosome has 80 different protein components.

Like structure, the composition of NPC is highly conserved between human and yeast.


Schematic illustration of the NPC structure

Angelo & Hetzer, 2008 (Review)


Predicted composition of scaffold and central channel

Angelo & Hetzer, 2008 (Review)


Predicted composition of cytoplasmic components

Angelo & Hetzer, 2008 (Review)


Predicted composition of nuclear components

Angelo & Hetzer, 2008 (Review)


Predicted composition of transmembrane components

Angelo & Hetzer, 2008 (Review)


Predicted localization of sub-complexes and nucleoporins within the NPC

Angelo & Hetzer, 2008 (Review)


Summary

The nuclear pore complex in turn is made of several protein sub-complexes.

The NPC sub-complexes are divided to two categories:1) peripheral 2) central


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