Structure and composition of nuclear pore complexes npcs
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Structure and composition of nuclear pore complexes (NPCs). Electron micrographs of NPCs. Gall, 1967. Electron micrographs of NPCs. Gall, 1967. Three-dimensional view of a nuclear pore in the double-layered nuclear envelope. Gall, 1967.

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Structure and composition of nuclear pore complexes (NPCs)

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Structure and composition of nuclear pore complexes npcs

Structure and composition of nuclear pore complexes (NPCs)


Structure and composition of nuclear pore complexes npcs

Electron micrographs of NPCs

Gall, 1967


Structure and composition of nuclear pore complexes npcs

Electron micrographs of NPCs

Gall, 1967


Structure and composition of nuclear pore complexes npcs

Three-dimensional view of a nuclear pore in the double-layered nuclear envelope

Gall, 1967


Structure and composition of nuclear pore complexes npcs

Diagram of a model cylinder with the x-, y- and z-axes

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

Averaged xy slices taken from the 3D map of the NPC

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D side view of the NPC surface map

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

z slices taken from 3D maps of the NPCs

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

Surface views of the NPC 3D map

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D representation of the membrane-associated NPC

Akey & Radermacher, 1993


Structure and composition of nuclear pore complexes npcs

3D structure of the NPC

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

Cutaway view of the NPC

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

Slices through the structure of the pore

Beck et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC nuclear basket

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC cytoplasmic particles

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

The NPC cytoplasmic particles

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

Immuno-labelling of Nup116

Kiseleva et al, 2004


Structure and composition of nuclear pore complexes npcs

Summary

The NPC has eight rotational symmetry.

The NPC is constructed from three main parts: 1) cytoplasmic components 2) central parts3) nucleoplasmic components

The NPC is huge structure. The vertebrate NPC has about 125 nm diameter (around 20 times larger than a ribosome).


Structure and composition of nuclear pore complexes npcs

Comparison of the peripheral and intrinsic membrane polypeptides of nuclear envelopes and microsomal membranes

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Characterization of antibodies to gp 190 and the lamins

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Immunofluorescence staining of tissue culture cells with anti-gp 190 and anti-lamins A and C

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Immuno-labeling of nuclei with anti-gp 190

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Presence of gp 190 in diverse vertebrate species

Gerace et al, 1982


Structure and composition of nuclear pore complexes npcs

Mammalian, yeast and worm nucleoporins

Angelo & Hetzer, 2008 (Review)


Structure and composition of nuclear pore complexes npcs

Summary

The NPC is composed of ONLY about 30 different proteins (nucleoporins). Ribosome has 80 different protein components.

Like structure, the composition of NPC is highly conserved between human and yeast.


Structure and composition of nuclear pore complexes npcs

Schematic illustration of the NPC structure

Angelo & Hetzer, 2008 (Review)


Predicted composition of scaffold and central channel

Predicted composition of scaffold and central channel

Angelo & Hetzer, 2008 (Review)


Predicted composition of cytoplasmic components

Predicted composition of cytoplasmic components

Angelo & Hetzer, 2008 (Review)


Predicted composition of nuclear components

Predicted composition of nuclear components

Angelo & Hetzer, 2008 (Review)


Predicted composition of transmembrane components

Predicted composition of transmembrane components

Angelo & Hetzer, 2008 (Review)


Predicted localization of sub complexes and nucleoporins within the npc

Predicted localization of sub-complexes and nucleoporins within the NPC

Angelo & Hetzer, 2008 (Review)


Structure and composition of nuclear pore complexes npcs

Summary

The nuclear pore complex in turn is made of several protein sub-complexes.

The NPC sub-complexes are divided to two categories:1) peripheral 2) central


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