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PCR = DNA ?. LB 145 January 27, 2011. Does PCR happen inside your body? (How do you raise your temp to 95°C?) PCR was invented in the last 30 years. It exploits our knowledge of another process called ??. Through the process of DNA Replication. Why?.

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pcr dna

PCR = DNA ?

LB 145

January 27, 2011

slide2
Does PCR happen inside your body?

(How do you raise your temp to 95°C?)

  • PCR was invented in the last 30 years. It exploits our knowledge of another process called ??...
slide4
Why?
  • Each cell must have a way to copy its entire genome
    • Cell Division
    • Damage Control

Where does this take place?

check this out
Check this out
  • http://www.youtube.com/watch?v=teV62zrm2P0&feature=related
  • http://www.youtube.com/watch?v=AGUuX4PGlCc
pcr vs dna replication
PCR vs. DNA Replication
  • Similarities?
    • Copy DNA
    • Move 5’-3’ via a polymerase enzyme
    • require primers
pcr vs dna replication1
PCR vs. DNA Replication
  • Differences?
    • Constant temperature (body temp is 37C)
      • Enzymes are the workhorses
    • All 3 stages happen simultaneously
    • Multiple enzymes vs. Taq Polymerase
    • Leading/Lagging Strand
    • RNA primers vs DNA primers
so what are we doing again
So, what are we doing again?
  • PCR is selective DNA replication, it exploits our knowledge of the DNA rep.
  • Instead of using difficult (and expensive) enzymes in the body we used more controllable, predictable PCR machines.
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