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PCR = DNA ?. LB 145 January 27, 2011. Does PCR happen inside your body? (How do you raise your temp to 95°C?) PCR was invented in the last 30 years. It exploits our knowledge of another process called ??. Through the process of DNA Replication. Why?.

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Pcr dna

PCR = DNA ?

LB 145

January 27, 2011


Pcr dna

  • Does PCR happen inside your body?

    (How do you raise your temp to 95°C?)

  • PCR was invented in the last 30 years. It exploits our knowledge of another process called ??...


Pcr dna

  • Through the process of DNA Replication


Pcr dna

Why?

  • Each cell must have a way to copy its entire genome

    • Cell Division

    • Damage Control

      Where does this take place?


Check this out

Check this out

  • http://www.youtube.com/watch?v=teV62zrm2P0&feature=related

  • http://www.youtube.com/watch?v=AGUuX4PGlCc


Pcr vs dna replication

PCR vs. DNA Replication

  • Similarities?

    • Copy DNA

    • Move 5’-3’ via a polymerase enzyme

    • require primers


Pcr vs dna replication1

PCR vs. DNA Replication

  • Differences?

    • Constant temperature (body temp is 37C)

      • Enzymes are the workhorses

    • All 3 stages happen simultaneously

    • Multiple enzymes vs. Taq Polymerase

    • Leading/Lagging Strand

    • RNA primers vs DNA primers


So what are we doing again

So, what are we doing again?

  • PCR is selective DNA replication, it exploits our knowledge of the DNA rep.

  • Instead of using difficult (and expensive) enzymes in the body we used more controllable, predictable PCR machines.


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