Introduction Arabidopsis thaliana is widely studied due to its small genome and the many mutant lines available (Rédei, 1969, Bowman, 1994).
Arabidopsis thaliana is widely studied due to its small genome and the many mutant lines available (Rédei, 1969, Bowman, 1994).
The first quantitative study and detailed observations of ovule development of megagametophytic stages was of five Cornus L. species using a clearing technique (Smith, 1973, Smith 1975) .
Clearing techniques allows you to halt the development of ovaries in certain stages and observe them with clarity using phase contrast microscopy.
Morphological size disparities in the different developmental stages could be detected by calculating Fibonacci sequences in successive stages
Many life-forms have the golden ratio in some aspects of their design e.g., flowering plants have petals that are fibonacci numbers such lilies, iris, corn, asters, etc
To obtain the Mean and CI of the length and width of megasporogenesis and megagametogenesis stages of A. thaliana .
To use the means of the widths and lengths to see if the Fibonacci sequence can be determined (the golden ratio, phi)
Methods and Materials
Measuring of Length and Width of Ovules from Arabidopsis thaliana (L.) HEYNH. undergoing Megagametogenesis and determining the Fibonacci Sequence using the MeanAshad Syed* and Bruce SmithYork College of PA
A. thaliana, Landsbergecotype
grown from seeds
Fixed for 24 hrs in FPA50 and then treated
with ETOH dehydration treatment with 10-15 min exposure to each fluid
70% →80% →90% →95% →100%
Added to Herr Fluid
Observed under phase contrast microscope at 100x magnification
Measure length and width using software
Compute data to determine
if Fibonacci sequence can be obtained (Golden Ratio)