Schott nexterion mtp 96
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SCHOTT NEXTERION MTP-96. Maximum & Minimum filling volumes per well. Experimental Details. Substrate tested: - MTP 2-2259 without patterning 18.01.11 Buffer Solutions tested: - PBS (1x) with 0.08 mg/ml Dye 5 - PBST 0.1% Tween with 0.08 mg/ml Dye 5. Filling Scheme.

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SCHOTT NEXTERION MTP-96

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Schott nexterion mtp 96

SCHOTT NEXTERION MTP-96

Maximum & Minimum filling volumes per well


Experimental details

Experimental Details

  • Substrate tested:

    - MTP 2-2259 without patterning 18.01.11

  • Buffer Solutions tested:

    - PBS (1x) with 0.08 mg/ml Dye 5

    - PBST 0.1% Tween with 0.08 mg/ml Dye 5

Corinna Schwung, 22.08.2014


Filling scheme

Filling Scheme

These wells were filled up to maximum liquid level.

Corinna Schwung, 22.08.2014


Results from adding 10 120 l per well

1

2

3

4

5

6

7

8

9

10

11

12

A

B

C

D

E

F

G

H

Results from adding 10 – 120 µl per well

  • Solution pipetted into upper left corner

  • MTP was gently shaken

PBST

  • Minimum Volumes

  • PBST: 40 µl

  • PBS: 50 µl

PBS

10 µl

120 µl

Corinna Schwung, 22.08.2014


Results from adding 10 240 l per well

1

2

3

4

5

6

7

8

9

10

11

12

A

B

C

D

E

F

G

H

Results from adding 10 – 240 µl per well

130 µl

240 µl

PBST

PBST

10 µl

120 µl

PBS

PBS

130 µl

240 µl

Corinna Schwung, 22.08.2014


Results from adding 10 240 l per well1

Results from adding 10 – 240 µl per well

  • Safe filling volume is about 160 µl for PBS, 130 µl for PBST

PBST

PBS

240 µl

130 µl

140 µl

150 µl

160 µl

170 µl

180 µl

190 µl

200 µl

210 µl

220 µl

230 µl

PBS

PBST

240 µl

230 µl

220 µl

210 µl

200 µl

190 µl

180 µl

170 µl

160 µl

150 µl

140 µl

130 µl

Corinna Schwung, 22.08.2014


Results from adding 10 240 l per well2

Results from adding 10 – 240 µl per well

PBS

190 µl

200 µl

210 µl

220 µl

230 µl

240 µl

  • The maximum filling level was not reached with these volumes

PBST

240 µl

230 µl

220 µl

210 µl

200 µl

190 µl

Corinna Schwung, 22.08.2014


Results from adding 320 400 l per well

Results from adding 320 – 400 µl per well

PBS

  • Maximum Volume per well

  • PBS: 410 µl

  • PBST: 340 µl

370 µl

380 µl

390 µl

400 µl

PBST

350 µl

340 µl

330 µl

320 µl

Corinna Schwung, 22.08.2014


Conclusions

Conclusions

  • The minimum volume that will cover the well bottom is:

    • 40 microlitres (with detergent)

    • 50 microlitres (without detergent)

  • The maximum recommended „safe“ working volume is:

    • 130 microlitres (with detergent)

    • 160 microlitres (without detergent)

Corinna Schwung, 22.08.2014


Schott nexterion mtp 961

SCHOTT NEXTERION MTP-96

Maximum & Minimum filling volumes per well


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