Alzheimer s disease
This presentation is the property of its rightful owner.
Sponsored Links
1 / 16

Alzheimer’s Disease PowerPoint PPT Presentation


  • 77 Views
  • Uploaded on
  • Presentation posted in: General

Alzheimer’s Disease. BRAIN, THE FINAL FRONTIER. Kiminobu Sugaya, Ph.D. Alzheimer’s Disease. NH 2. COOH. A . Plaques. Normal Secretory Pathway. Amyloid- b precursor protein. b -secretase.  -secretase. g -secretase.  -amyloid deposition.

Download Presentation

Alzheimer’s Disease

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Alzheimer s disease

Alzheimer’s Disease

BRAIN,

THE FINAL FRONTIER

Kiminobu Sugaya, Ph.D.


Alzheimer s disease1

Alzheimer’s Disease


Amyloid b precursor protein

NH2

COOH

A

Plaques

Normal Secretory Pathway

Amyloid-b precursor protein

b-secretase

-secretase

g-secretase

-amyloid deposition


Mechanism of neurodegeneration in alzheimer s disease

Mechanism of Neurodegeneration in Alzheimer’s Disease

APP

Glial Activation

Presenilin

Presenilin

Ab

1-40, 1-42

Neurodegeneration

Amyloid

Deposition


Glial activation in aging and disease

Glial Activation in Aging and Disease

Neuronal Dysfunction or Neurodegeneration

Glial Activation (Inflammation)

Shift

Shift

Age


Alzheimer s disease

DNA Fragmentation

Cromatin Condensation

Membrane Blebbing and Loss of Nuclear Envelope

Cellular Fragmentation into Apoptotic Bodies

APOPTOSIS

APOPTOSIS

Phagocytosis and Degradation


Fragmented dna apoptosis in the hippocampus

Alzheimer’s disease

Fragmented DNA (Apoptosis) in the hippocampus

Aged Control


Amyloid plaque and apoptosis

Plaque with many

Plaque with many

TUNEL

positive

TUNEL

positive

cells

cells

Plaque with no

Plaque with no

TUNEL

positive

TUNEL

positive

cells

cells

Plaque with a few TUNEL

positive cells

Plaque with a few TUNEL

positive cells

Amyloid Plaque and Apoptosis


Neuro fibrillary tangles and apoptosis

Neuro-fibrillary Tangles and Apoptosis


Astrocyte and apoptosis

Apoptotic Cells

Astorocyte

Astrocyte and Apoptosis


Gfap mrna expression in the aged memory impaired model rat

Ci/g tissue

14.68

68.42

122.16

175.90

229.64

283.38

337.13

390.87

444.61

600

Young

498.35

552.09

605.83

young

500

659.57

Aged unimpaired

713.31

Aged impaired

767.05

820.79

400

874.54

928.28

982.02

300

1035.76

Radio activity in the section (nCi/g)

1089.50

1

1

4

3

.

2

4

Aged unimpaired

1

1

9

6

.

9

8

200

1250.72

p=0.0001

1304.46

1358.20

100

1

4

1

1

.

9

4

1465.69

1519.43

0

1573.17

1626.91

300

120

140

160

180

200

220

240

260

280

1680.65

1734.39

memory score

Aged impaired

Regression Analysis of GFAP mRNA Expression in the Hippocampus against the Memory Score

GFAP mRNA Expression in the Aged Memory Impaired Model Rat


App mrna expression in the aged memory impaired model rat

68.17

176.38

284.60

392.81

501.03

609.24

717.46

825.67

933.89

1042.10

1

150.31

1258.53

1366.74

young

2000

1474.96

Aged unimpaired

1583.17

Aged impaired

1900

1691.39

1799.60

1800

1907.81

1700

2016.03

2124.24

1600

2232.46

Radio activity in the section (nCi/g)

2340.67

1500

2448.89

1400

2557.10

2665.32

1300

2773.53

2881.74

1200

p = 0.0055

2989.96

1100

3098.17

3206.39

3314.60

3422.82

120

140

160

180

200

220

240

260

280

300

3531.03

memory score

Ci/g tissue

Regression Analysis of APP mRNA Expression in the Hippocampus against the Memory Score

APP mRNA Expression in the Aged Memory Impaired Model Rat

Young

Aged unimpaired

Aged impaired


Mn sod mrna expression in the aged memory impaired model rat

210

young

Aged unimpaired

190

Aged impaired

170

150

Radio activity in the section (nCi/g)

130

110

p = 0.0190

90

120

140

160

180

200

220

240

260

280

300

memory score

Ci/g tissue

Regression Analysis of Mn-SOD mRNA Expression in the Hippocampus against the Memory Score

49.28

52.13

54.97

57.82

60.66

63.51

66.36

69.20

72.05

74.89

77.74

80.59

83.43

86.28

89.12

91.97

94.82

97.66

100.51

103.35

106.20

109.04

111.89

114.74

117.58

120.43

123.27

126.12

128.97

131.81

134.66

137.50

Mn-SOD mRNA Expression in the Aged Memory Impaired Model Rat

Young

Aged unimpaired

Aged impaired


App ishh with gfap immunostaining in the aged memory impaired model rats

Hilus

CA1

Dentate Gyrus

CA1

Dentate Gyrus

Hilus

APP ISHH with GFAP Immunostaining in the Aged Memory Impaired Model Rats

Aged Impaired

matured


Gfap ishh astrocytes marker with 4g8 a b immuno histochemistry in ps1 app tm

GFAP ISHH (astrocytes marker) with 4G8 (Ab) Immuno-histochemistry in PS1-APP TM


App ishh with gfap immuno histochemistry astrocytes marker in ps1 app tm

APP ISHH with 4G8 (Ab) Immuno-histochemistry in PS1-APP TM

APP ISHH with GFAP Immuno-histochemistry (astrocytes marker) in PS1-APP TM


  • Login