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MICROBIAL GROWTH. By Dr. Ijeoma Nkeiruka Otigbuo. PHYSICAL REQUIREMENTS. Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs. pH: Acidophiles, Extreme halophiles, and facultative halophiles. CHEMICAL REQUIREMENTS. Carbon. Nitrogen, Sulfur, and Phosphorus.

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Microbial growth l.jpg

MICROBIAL GROWTH.

By

Dr. Ijeoma Nkeiruka Otigbuo.


Physical requirements l.jpg
PHYSICAL REQUIREMENTS.

  • Temperature:Psychrophiles, mesophiles, thermophiles, and psychrotrophs.

  • pH: Acidophiles, Extreme halophiles, and facultative halophiles.


Chemical requirements l.jpg
CHEMICAL REQUIREMENTS.

  • Carbon.

  • Nitrogen, Sulfur, and Phosphorus.

  • Trace Elements.

  • Oxygen: Obligate aerobes, Facultative anaerobes, Obligate anaerobes, Aerotolerant anaerobes, and Microaerophiles.

  • Organic Growth Factors.


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TOXIC FORMS OF OXYGEN.

  • Singlet oxygen.

  • Superoxide free radicals.

  • The hydroxyl radical.


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CULTURE MEDIA.

  • Chemically Defined Media.

  • Complex Media.

  • Anaerobic Growth Media and Methods.

  • Special Culture Techniques.

  • Selective and Differential Media.

  • Enrichment Culture.


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OBTAINING PURE CULTURES.

  • Streak Plate Method.


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PRESERVING BACTERIAL CULTURES.

  • Deep Freezing.

  • Lyophilization.

  • Cryopreservation.


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GROWTH OF BACTERIAL CULTURES.

  • Bacterial Division.

  • Generation Time.

  • Phases of Growth: Lag, Log,Stationary, and Death.


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DIRECT MEASUREMENT OF MICROBIAL GROTW.

  • Pate Counts.

  • Serial Dilutions.

  • Pour Plates and Spread Plates.

  • Filtration.

  • Most Probable Number Method.

  • Direct Microscopic Count.


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ESTIMATION OF NUMBERS BY INDIRECT METHODS.

  • Turbity.

  • Metabolic Activity.

  • Dry Weight.


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