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Supplementary Figure 1. B. Control X0. 100 90 80 70 60 50 40 30 20 10. a - HBx ( mAb ). Control X9. a - HBx (rabbit serum). C. A. B. C. E. F. 0. X0. X0. X0. X0. X0. X0. X0. X0. X9. X9. X9. X9. X9. X9. X9. X9. Control. Control. Control. Control.

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Supplementary figure 1

Supplementary Figure 1

B

Control X0

100

90

80

70

60

50

40

30

20

10

a-HBx

(mAb)

Control X9

a-HBx

(rabbit serum)

C

A

B

C

E

F

0

X0

X0

X0

X0

X0

X0

X0

X0

X9

X9

X9

X9

X9

X9

X9

X9

Control

Control

Control

Control

Control

Control

Control

Control

Cells (%)

A

1 58 85 120 154 aa

A

B

C

D

E

F

X0

X9 (DD)

0 1 2 3 4 6 8 10

Post Double Thymidine Block (h)


Supplementary figure 1

Supplementary Figure 2

A

+ 1

AP-2

CCAAT

NFkb

+ 225

-1534

E2F1

E2F1

CDC6 promoter

[-1534, +225]

- 100

- 200

-130

Wild type E2F1 binding site

TTTGGCGC

Luciferase

E2F1

E2F1

CDC6 WT

-130

Mutant E2F1 binding site

TTTAACGC

E2F1

E2F1

Luciferase

CDC6-SM1

B

#

#

Control

X0

Normalized luciferase units

pGL3 CDC6 WT CDC6-SM1

C

Cyclin A

Binding

(68-108)

DP-1

Binding

(206-283)

DNA

Binding

(120-191)

Rb

binding

Transactivation

Domain

(323-437)

Wild-type

E2F1-WT

Transactivation

Domainmutant

E2F1 (1-374)


Supplementary figure 1

Supplementary Figure 3

+ 1

CCAAT

CCAAT

CCAAT

Sp1

Sp1

Sp1

Sp1

Sp1

-728

+ 127

E2F1 promoter

[-728, +127]

E2F1

E2F1

- 122

- 204

- 242

- 242

Luciferase

E2F1

E2F1

pGL3-E2F1 WT

- 242

Luciferase

E2F1

E2F1

pGL3-E2F1 Mut


Supplementary figure 1

Supplementary Figure 4

A

Human b-globin origin

Relative abundance of CDC6

on human b-globin origin of replication

(arbitrary unit)

ChIP

Control X0 X9

Control X0 X9

a-CDC6

Control

Serum Stimulation (h)

Input

3h 7h

Serum Stimulation

B

g-globulin non-origin locus

Control X0 X9

Control X0 X9

ChIP

a-CDC6

Relative abundance of CDC6

on human g-globulin locus

(arbitrary unit)

Control

Input

3h 7h

Serum Stimulation

Serum Stimulation (h)


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