Peg protocol
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PEG protocol. as of April 2005. Cleaning, 2 hrs. Step A (Slides). Step B (Coverslips). Step C (Reaction containers). Step D, 1hr (Aminosilanization). Step E, 2hr incubation (PEGylation). Notes.

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PEG protocol

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Peg protocol

PEG protocol

as of April 2005


Peg protocol

Cleaning, 2 hrs

Step A

(Slides)

Step B

(Coverslips)

Step C

(Reaction containers)

Step D, 1hr

(Aminosilanization)

Step E, 2hr incubation

(PEGylation)


Notes

Notes

  • Reaction containers: any glass containers which are used for aminosilanization. We recycle them. We use a couple of glass staining dishes and a flask.

  • Aminosilane: obtained from UCT, Cat. # A0700, 25mg, expire after six months

  • PEG: obtained from Nektar Therapeutics. biotin-PEG (0H4M0H02, BIO-NHS-5000), mPEG (2M4M0H01, M-SPA-5000)

  • Methanol: ACS grade

  • The other chemicals such as sodium bicarbonate: from Fisher

  • The diameter of our centrifuge is 5ÔÇŁ. The power of our sonicator is 130W.


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