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Max-Planck-Institute of Plant Breeding

Max-Planck-Institute of Plant Breeding. Dept. of Plant Microbe Interactions (P. Schulze-Lefert). Research areas: -Recognition and Signalling in Disease Resistance (Paul Schulze-Lefert) Molecular Mechanisms of Defence Suppression (Ralph Panstruga)

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Max-Planck-Institute of Plant Breeding

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  1. Max-Planck-Institute of Plant Breeding Dept. of Plant Microbe Interactions (P. Schulze-Lefert) • Research areas: • -Recognition and Signalling in Disease Resistance (Paul Schulze-Lefert) • Molecular Mechanisms of Defence Suppression (Ralph Panstruga) • Signaling mechanisms in Plant Innate Immunity (Jane Parker) • Molecular basis of Fungal Biotrophy (Richard O'Connell) • Regulatory Protein Complexes in Plant Defense (Erich Kombrink) • Transcriptional Regulation in Plant Defence (Imre E. Somssich) • The Mechanism of FL2 Signaling in Plant Innate Immunity (Silke Robatzek) • Functional Genomics in Barley (Thomas Koprek); until end of 2005

  2. Mutations in barley Mlo confer resistance to all isolates of Blumeria graminis f. sp. hordei Mlo wildtype mlo mutant BUT:mlo mutants are more susceptible to certain hemibiotrophic (Magnaporthe grisea) and necrotrophic (Bipolaris sorokiniana) fungi

  3. TPS 8, 263, 2003

  4. Inducibility and expression kinetics of parsley genes upon Pmg elicitor treatment A B probe: nuclear run-on transcript

  5. Fungal Elicitor Responsive Cis-acting Elements W1 box CTTACACACTTAATTTGACCGAGTAACATTCG W2 box CAATTACAGCCATCAAAGTTGACCAATAATA W3 box CTATTATGACTAAATAGTCAGAATTTCCAA E17 box TGTCAATGGTCAACATTCAAC D box TACAATTCAAACATTGTTCAAACAAGGAACC U box TGTGCCATGAAGTTGAAATTCAATAGTGTGC S box CAGCCACCAAAGAGGACCCAGAAT GCC box CAGCCGCCAAAGAGGACCCAGAAT Gst1 box TTCTAGCACCAGATTTGACCAAAC Rushton et al., Plant Cell l4, 749 (2002)

  6. TATA-box cis-element 4 x W1 box 4 x D box MgCl P. syringae 2 x (W2/S/ D) box 4 x S box B. graminis P. parasitica

  7. The WRKY family of transcription factors Group I WRKY C-C-H-H WRKY C-C-H-H Group II C-C-H-H WRKY HARF WRKY C-C-H-H WRKY C-C-H-H LZ Group III WRKY C-C- -H-C WRKY C-C- -H-C LZ WRKY domain:

  8. The WRKY Family of Transcription Factors • Novel Zn finger type DNA binding proteins • Translocated into the plant cell nucleus via NLS motif • Bind to cis-regulatory element:W box (C/T TGAC T/C) • Phosphorylation increases DNA binding affinity • Contain functional transactivation domains • Capable of activating but also repressing gene expression AGTTGACCAA AGATGACCAA AGGTGACCAA AGCTGACCAA AGTTGACAAA AGTTGACGAA AGTTGACTAA AGTAGACCAA AGTTCACCAA AGTTGAACAA AGTTGACCAA WRKY11 At4g31550

  9. WRKY genes are found outside of the plant phyla

  10. Arabidopsis thaliana WRKY Family

  11. AtWRKY27 KO mutant Wt Response of AtWRKY KO lines to Ralstonia solanacearum 8 days post root inoculation with strain GMI1000 (virulent on wt Col-0 plants)

  12. W27ETL-Col-1 AtWRKY27 KO lines show enhanced resistance towards Rastonia solanacearum (GMI1000) infection Control-Col-0 4 Control-Col-1 W27-SALK-Col-0 3 Disease Index 2 1 0 0 2 4 6 8 10 12 14 Days post-inoculation (50 plants from each line root inoculated) Disease Index 2 : 25 – 50 % wilted leaves 0 : no wilt symptom 3 : 50 – 75 % wilted leaves 1 : 0 – 25 % wilted leaves 4 : 75 – 100 % wilted leaves

  13. Col-0 WRKY40 14 WRKY18 12 10 WRKY53 8 bacteria log cfu/cm2 6 4 2 0 Days 0 2 4 AtWRKY40 + 53 knockout lines are more susceptible towards P. syringae pv. tomato DC3000 Col-0 WRKY40 KO WRKY53 KO

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