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P04637|Human KGEPHHELPPGSTKRALPNNTSS S PQPKKKPLDGEYFTLQIRGRERFEMFRE

315. P04637|Human KGEPHHELPPGSTKRALPNNTSS S PQPKKKPLDGEYFTLQIRGRERFEMFRE P56424|Rhesus KGEPCHQLPPGSTKRALPNNTSS S PQPKKKPLDGEYFTLQIRGRERFEMFRE

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P04637|Human KGEPHHELPPGSTKRALPNNTSS S PQPKKKPLDGEYFTLQIRGRERFEMFRE

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  1. 315 P04637|Human KGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRE P56424|Rhesus KGEPCHQLPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRE P67939|Cow KGQSCPEPPPRSTKRALPTNTSSSPQPKKKPLDGEYFTLQIRGFKRYEMFRE P51664|Sheep KGQSCPEPPPGSTKRALPSSTSSSPQQKKKPLDGEYFTLQIRGRKRFEMFRE Q9TUB2|Pig KGQSCPEPPPGSTKRALPTSTSSSPVQKKKPLDGEYFTLQIRGRERFEMFRE Q9WUR6|Guinea KGGLCPEPTPGNIKRALPTSTSSSPQPKKKPLDAEYFTLKIRGRKNFEILRE P02340|Mouse KEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRE P10361|Rat KEEHCPELPPGSAKRALPTSTSSSPQQKKKPLDGEYFTLKIRGRERFEMFRE O09185|Chinese Ham KGEPCPELPPKSAKRALPTNTSSSPPPKKKTLDGEYFTLKIRGHERFKMFQE A B untransfected p53 p53S312A 0 3 6 0 3 6 TG (hr): p53 phos-S312 NS p53 Actin Supplementary Figure 1 – Lee et al.

  2. A p53 +/+ p53 +/+ p53S312A/S312A p53S312A/S312A p53-/- p53-/- 0 0 2 2 4 4 6 6 0 0 2 2 4 4 6 6 N.S. p53phos-S15 p53 Actin MEFs Thymocytes B 3 weeks old 5 months old p53-/- IR (hr): 0 2 5 0 2 5 p53 Actin Supplementary Figure 2 – Lee et al.

  3. Time (hrs): 0 4 8 12 15 24 22.57% p53 +/+ 23.15% 35.05% 28.74% 28.36% 30.65% 20.76% 21.76% 32.61% 27.8% 27.96% 27.38% p53 S312A/S312A 40.69% 42.83% 54.11% 46.34% 43.21% 50.35% p53 -/- BrdU DNA content A B Supplementary Figure 3A-B – Lee et al.

  4. 1.2 1.2 1.2 1 1 1 0.8 0.8 0.8 relative expressin of mdm2 relative expression of noxa 0.6 0.6 0.6 relative expression of p21 0.4 0.4 0.4 0.2 0.2 0.2 0 0 0 0 5 10 15 20 25 30 0 5 10 15 20 25 30 0 5 10 15 20 25 30 Time (hr after serum release) Time (hr after serum release) Time (hr after serum release) 5 6 4.5 4 5 3.5 4 3 Fold induction of cyclin A2 2.5 Fold Induction of cyclin D1 WT 3 2 1.5 2 1 0.5 1 0 0 0 5 10 15 20 25 30 0 5 10 15 20 25 30 Time (hr after serum release) Time (hr after serum release) C Supplementary Figure 3C – Lee et al.

  5. Each dot represent 1 gene Supplementary Figure 4 – Lee et al.

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