Antibody Titration. Mr . Mohammed A. Jaber. Principle. Titration is a semiquantitative method used to determine the concentration of antibody in a serum sample or to compare the strength of antigen expression on different red cell samples. The usual applications of titration studies are:
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Mr . Mohammed A. Jaber
Titration studies specifically to assist in monitoring clinically significant antibodies in the pregnant woman.
+4 Single clump of agglutination with no free cells
+3 Three or four individual clumps with few free cells
+2 Many fairly large clumps with many free cells
+1 Fine granular appearance visually, but definite small clumps (10-15 cells) per low power field
+W 2 to 3 cells sticking together per low power field, uneven distribution Visually no agglutination
- All cells are free
H Hemolysis (partial or total) must be interpreted as positive