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PROTEIN ENGINEERING. Loschmidt Laboratories Enantis , Ltd. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team

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PROTEIN ENGINEERING

LoschmidtLaboratories

Enantis, Ltd.


LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science

  • multi-disciplinary team


LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science

  • multi-disciplinary team

  • laboratories and facilities(1.5 mil EUR)University Campusand Biotechnology Incubator, Brno


LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science

  • multi-disciplinary team

  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno

    • molecular biology andmicrobiology


LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science

  • multi-disciplinary team

  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno

    • molecular biology andmicrobiology

    • bioinformaticsandmolecular modelling


LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science

  • multi-disciplinary team

  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno

    • molecular biology andmicrobiology

    • bioinformaticsandmolecular modelling

    • biochemistryandbiophysics


LOSCHMIDT LABORATORIES & ENANTIS

BIOSENSING

BIOCATALYSIS

DECONTAMINATION



DEVELOPMENT OF BIOCATALYTIC PROCESS

  • GENETIC DATABASES

  • EXTREMOPHILES


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION

  • resistance to organicsolvents


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION

  • RATIONAL DESIGN

  • thermostabilityenhancement


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION

  • RATIONAL DESIGN

  • thermostabilityenhancement


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION

  • RATIONAL DESIGN

  • thermostabilityenhancement

  • 20°C


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • OPTIMIZED EXPRESSION

  • FERMENTATION

  • DOWN STREAM


DEVELOPMENT OF BIOCATALYTIC PROCESS

  • MEDIUM ENGINEERING

  • IMMOBILISATION


HOTSPOT WIZARD

RCSB PDB

Hot Spot

Wizard

user


HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

Hot Spot

Wizard

user


HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

CASTp

Hot Spot

Wizard

user

CAVER


HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

CASTp

Hot Spot

Wizard

user

CAVER

Rate4Site

Cd-hit

MUSCLE

tunnels

BLAST

NRDB


low

high

mutability

HOTSPOT WIZARD


HOTSPOT WIZARD

haloalkanedehalogenase

PDB-ID 1BN6

1,3-1,4-b-glucanase

PDB-ID 1GBG

b-lactamase

PDB-ID 1BLS

phosphotriesterase

PDB-ID 1I0D


HOTSPOT WIZARD

positive and neutral

hot spots

region

Proportion of mutations

Estimated mutability


HOTSPOT WIZARD

positive and neutral

slightly negative

hot spots

region

Proportion of mutations

Estimated mutability


HOTSPOT WIZARD

positive and neutral

slightly negative

deleterious

hot spots

region

Proportion of mutations

Estimated mutability


HOTSPOT WIZARD

Pavelkaet al. Nucl. Acids Res. 37: W376 2009


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