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PROTEIN ENGINEERING. Loschmidt Laboratories Enantis , Ltd. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team. LOSCHMIDT LABORATORIES & ENANTIS. 17 years experience in protein science multi - disciplinary team

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Presentation Transcript
slide1

PROTEIN ENGINEERING

LoschmidtLaboratories

Enantis, Ltd.

slide2

LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science
  • multi-disciplinary team
slide3

LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science
  • multi-disciplinary team
  • laboratories and facilities(1.5 mil EUR)University Campusand Biotechnology Incubator, Brno
slide4

LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science
  • multi-disciplinary team
  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno
    • molecular biology andmicrobiology
slide5

LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science
  • multi-disciplinary team
  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno
    • molecular biology andmicrobiology
    • bioinformaticsandmolecular modelling
slide6

LOSCHMIDT LABORATORIES & ENANTIS

  • 17 years experience in protein science
  • multi-disciplinary team
  • laboratories and facilities(1.5 mil EUR) University Campusand Biotechnology Incubator, Brno
    • molecular biology andmicrobiology
    • bioinformaticsandmolecular modelling
    • biochemistryandbiophysics
slide7

LOSCHMIDT LABORATORIES & ENANTIS

BIOSENSING

BIOCATALYSIS

DECONTAMINATION

slide9

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • GENETIC DATABASES
  • EXTREMOPHILES
slide10

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION
  • resistance to organicsolvents
slide11

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION
  • RATIONAL DESIGN
  • thermostabilityenhancement
slide12

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION
  • RATIONAL DESIGN
  • thermostabilityenhancement
slide13

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • DIRECTED EVOLUTION
  • RATIONAL DESIGN
  • thermostabilityenhancement
  • 20°C
slide14

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • OPTIMIZED EXPRESSION
  • FERMENTATION
  • DOWN STREAM
slide15

DEVELOPMENT OF BIOCATALYTIC PROCESS

  • MEDIUM ENGINEERING
  • IMMOBILISATION
slide16

HOTSPOT WIZARD

RCSB PDB

Hot Spot

Wizard

user

slide17

HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

Hot Spot

Wizard

user

slide18

HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

CASTp

Hot Spot

Wizard

user

CAVER

slide19

HOTSPOT WIZARD

CSA

PDBSWS

UniProt

RCSB PDB

CASTp

Hot Spot

Wizard

user

CAVER

Rate4Site

Cd-hit

MUSCLE

tunnels

BLAST

NRDB

slide20

low

high

mutability

HOTSPOT WIZARD

slide21

HOTSPOT WIZARD

haloalkanedehalogenase

PDB-ID 1BN6

1,3-1,4-b-glucanase

PDB-ID 1GBG

b-lactamase

PDB-ID 1BLS

phosphotriesterase

PDB-ID 1I0D

slide22

HOTSPOT WIZARD

positive and neutral

hot spots

region

Proportion of mutations

Estimated mutability

slide23

HOTSPOT WIZARD

positive and neutral

slightly negative

hot spots

region

Proportion of mutations

Estimated mutability

slide24

HOTSPOT WIZARD

positive and neutral

slightly negative

deleterious

hot spots

region

Proportion of mutations

Estimated mutability

slide25

HOTSPOT WIZARD

Pavelkaet al. Nucl. Acids Res. 37: W376 2009

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