PhD Course . TOPICS IN (NANO) BIOTECHNOLOGY Lecture 6. 30th October, 2006. Overview . So we have looked at what is DNA and what is a gene. We also looked at DNA replication and protein synthesis, and the path from the gene to protein
TOPICS IN (NANO) BIOTECHNOLOGY
30th October, 2006
In 1971 Cohen, exploited the antibiotic resistance of the plasmids to selectively enrich offspring that contained cell propogating plasmids.
In the late 60s, it was shown that CaCl2 made the cells of E.coli permeable so that they could take up DNA, but could not grow E.coli cells with genetic property changes.
In late 1972, Berg reported on methods for joining fragments of DNA outside of cells using ligases.
Endonucleases, or restrictions enzymes, would however, provide the tool for linking DNA.
In Nov. 1972, Berg, Boyer and Cohen met up at a deli bar in Honololu, and discussed the endonuclease that Boyer was working on, and that night they
dreamed of the collaborative project that would be the true start of recombinant DNA technology. In March 1973, the pair produced DNA fragments using Boyer’s technique and joined them to plasmids using Berg’s technique, and then introduced them into bacteria using Cohen’s technique.
The first demonstration of DNA cloning had been achieved.
Vectors for gene cloning
- Hybrid molecules designed for use in multiple cell types
- Multiple ORIs allow replication in both prokaryotic and eukaryotic host cells allowing transfer between different cell types