1 / 10

Finding genetical modified contaminations (GMO) in groceries by using gel electrophoresis & PCR Biotechnologische

GMO. Finding genetical modified contaminations (GMO) in groceries by using gel electrophoresis & PCR Biotechnologisches Gymnasium Mettnau-Schule, Radolfzell . Structure. Experiment design Grocery samples tested 3. Results of the investigation of GMO food

jack
Download Presentation

Finding genetical modified contaminations (GMO) in groceries by using gel electrophoresis & PCR Biotechnologische

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. GMO Finding geneticalmodified contaminations (GMO) in groceries by using gel electrophoresis & PCR Biotechnologisches Gymnasium Mettnau-Schule, Radolfzell

  2. Structure • Experiment design • Grocery samples tested • 3. Results of the investigation of GMO food • - Germany ( 1st and 2nd analysis) • - USA • 4. Conclusion • 5. Suggestions and next steps

  3. Experiment design • Sample preparation2)PCR • 3)Gel electrophoresis 4)Analysis

  4. Grocery samples (N = 23) 3 11 2

  5. Results of the investigation of GMO in food origin GMO primer plant primer result valid 1st analysis Germany tofu 1 - - - +1) tofu 2 - - - +1) soy meal - - - - corn meal - + - + hamburger - - - +1) tobacco - - - - dog‘s feed - - - +1) popcorn Seeberger - + - + negative c. - - - -2) positive c. + + + + 1 = no chlorophyll 2 = DNA was digested

  6. 2nd analysis GMO primer plant primer result valid Germany rabbit’s feed Vitakraft - + - + biotofu, Edeka - - - + fresh corn leaves Gottmadingen - + - + fresh corn - - - ? bio-corn Seeberger - - - + polenta Kattus - - - + tortilla chips, Delvita - + - + soy sprouts, bamboo garden - - - + tobacco, Lucky Strike - - - + Results of the investigation of GMO in food

  7. USA GMO primer plant primer results valid America’s choice corn starch - + - + America’s choice muffin mix - + - + All natural corn starch - - - + Agro Pure corn starch - - - + Quaker, corn meal - + - + Aunt Jamina corn meal mix - + - + Jiffy corn muffin mix - + - + fresh corn leaves - + - + fresh corn - + - + negative c. - + - + positve c. + + + + Results of the investigation of GMO in food

  8. Conclusion

  9. Suggestions and next steps • DNA is digested without sample buffer , even when it‘s boiled : Buffer contains complexing substances for Ca2+ and Mg2+ • Gels with 3 % (w/v) agarose : tends to keep air bubbles • High concentration of agarose and buffer : the gel swells • - Problem: the wells will become smaller and smaller : difficult to load - remove comb just before loading • PCR is not influenced by any mistakes • For electrophoresis: put samples + control samples of the same primer together in 1 line • – you can save space! • Two combs fits in one gel – that saves space as well ! • => For statistic purpose : sent us more samples !!! • Thank you for your attention

More Related