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Examples of Synergy H4 Applications:

Examples of Synergy H4 Applications:. Immunoassays Kinase activity Metabolic activity Mitochondrial membrane potential NADH/NADPH Na + /H + exchange Nucleic acid quantification Oxidative burst Oxidation reactions PCR product quantification & quality Protease activity

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Examples of Synergy H4 Applications:

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  1. Examples of Synergy H4 Applications: • Immunoassays • Kinase activity • Metabolic activity • Mitochondrial membrane potential • NADH/NADPH • Na+/H+ exchange • Nucleic acid quantification • Oxidative burst • Oxidation reactions • PCR product quantification & quality • Protease activity • Protein quantification • Receptor binding studies • Reporter gene • Signal Transduction • Single Nucleotide Polymorphisms • Abs/FI spectral scanning • Toxicology • AlphaScreen/AlphaLISA • Apoptosis • Bacterial adhesion • BRET/BRET2 • Cell adhesion • Cell expression • Cell proliferation • Cell viability & Cytotoxicity • Chemotaxis • Colorimetric assays • Cytokine analysis • DNA quantification • eGFP quantification • ELISA • Enzyme activity • Enzyme kinetics • FRET • Heterogeneous TRF (DELFIA) • Homogeneous TRF (LANCE)

  2. Examples of Absorbance/Photometric Applications: • Assay Target • AlamarBlue® Cell Proliferation, Viability & Counting • Amplex® Red Enzyme Activity • phosphoELISA Kinases • Vybrant assay C12 Rezarurin Metabolic Labeling • Vybrant MTT Cell Proliferation Assay Kit Cell Counting • Lipid Peroxidation Assay Kit (using MDA) Oxidative Stress • Neutral Red Uptake Assay Cell Proliferation • CellTiter 96® Cell Proliferation Assay (MTT) Cell Proliferation • DC Protein, Bradford, Lowry & BCA Protein Assays Protein Quantification • OxiSelect™ Superoxide Dismutase Activity Assay Oxidative Stress • Pierce 660nm Protein Assay Protein Quantification • Glycoprotein Carbohydrate Estimation Kit Glycoprotein Quantification • Colorimetric Protease Assay Kit Protease • Micro BCA Protein Assay Kit Protein Quantification • FluoReporter Biotin-XX Protein Labelling Kit Biotin • Sulforhodamine B Assay Cell Proliferation • WST-1 Cell Proliferation Assay Cell Counting • Cell Counting Kit-8 (CCK-8) Cell Counting • IDEXX FlockChek™ Avian Influenza Multi-species ELISA Assay Avian Influenza • Melamine ELISA Assay Food Safety • CaspACE™ Assay Apoptosis • MTT Assay Cell Proliferation • Growth Assay (OD at 600nm) Cell Growth • Haemoglobin denaturation Assay Haemoglobin

  3. Examples of Fluorescence Intensity Applications: • Assay Target • Adapta® Universal Kinase Assay Kinases • AlamarBlue® Cell Proliferation, Viability & Counting • Amplex® Red Enzyme Activity • CyQuant® Cell Proliferation Assay Kit Cell Proliferation • EnzCheck Proteases • Fluo-4 AM GPCR • GeneBLAzer GPCR GPCR • LIVE/DEAD® Viability/Cytotoxicity Kit Apoptosis • NDP Sensor Assay Kinases • Omnia® Kinase Assays Kinases • PiPer™ Phosphate Assay Kit Phosphatases • SYTOX® stains Viability • Vivid® CYP450 Cytochrome P450 • Z'-LYTE™ Kinases • Quant-iT™ PicoGreen® dsDNA Assay Kit Nucleic Acid Quantification • Transcreener® GDP FI Assay GTPase • Fura-2 GPCR • Indo-1 GPCR • FluxOR™ Potassium Ion Channel Assay Kits Ion Channels • OxiSelect™ ROS Assay Kit Oxidative Stress • CellTiter-Fluor™ Cell Viability Assay Cell Viability • BD™ Ratiometric Calcium Assay Kit GPCR • FluoroBlok Cell Invasion Assays (using calcein AM) Cell Migration

  4. Examples of Luminescence Applications: • Assay Target • CellTiter-Glo® Luminescent Cell Cell Proliferation • Dual-Glo® Luciferase Assay Gene Expression • Dual-Luciferase® Reporter (DLR™) Assay Gene Expression • Bright-Glo™ Luciferase Assay Gene Expression • Steady-Glo® Luciferase Assay Gene Expression • ONE-Glo™ Luciferase Assay Gene Expression • P450-Glo™ Assay CYP Activities • ADP-Glo™ Assay Kinase • Caspase-Glo® 3/7 Assay Apoptosis • cAMP-Glo™ Assay GPCR • GloSensor™ cAMP Assay GPCR • Aequorin GPCR • Photina® GPCR • Britelite™ plus Reporter Gene Assay Gene Expression • Steadylite plus™ Reporter Gene Assay Gene Expression • Neolite Reporter Gene Assay Gene Expression • ATPlite™ Luminescence Assay Cell Proliferation/Kinases • aCella -TOX™ Bioluminescence Cytotoxicity Assay Cell Death • ToxiLight® Non-destructive Cytotoxicity BioAssay Kit Cell Death/Cell Proliferation • ATP quantification assay (luciferine-luciferase) ATP Quantification

  5. Examples of Time-Resolved Fluorescence Applications: • Assay Target • LanthaScreen™ Kinase Assay Kinases • LanthaScreen™ Cofactor Recruitment Assays Nuclear Receptors • LanthaScreen™ Cellular assay Kinases • Transcreener® ADP TR-FRET Assay Kinases • LANCE® cAMP Detection Kit GPCR • DELFIA® cAMP Kit GPCR • DELFIA® Tyrosine Kinase Kit Kinases • DELFIA® EuTDA Cell Cytotoxicity Kit Cytotoxicity • DELFIA® Cell Proliferation Kit Cell Proliferation • PI3-Kinase HTRF Assay Cell Proliferation • HTRF® HTplex™ cAMP and IP-One Assay GPCR • HTRF® cAMP femto 2 Kit GPCR • HTRF® KinEASE™ Kits Kinases • HTRF® heparanase Assay Heparanases • HTRF® Ubiquitin Assays Ubiquitinases • Human Insulin TRF-Pincer™ Assay Insulin • Tag-lite® Receptor Ligand Binding Assay GPCR • Cellul'erk GPCR • HTScan® Kinase Assays Kinases

  6. Examples of Fluorescence Polarization Applications: • Assay Target • PolarScreen™ Kinases • PolarScreen™ Competitive Binding Assays Nuclear Receptors • Transcreener® AMP/GMP Assay PDE’s • Transcreener® GDP FP Assay GTPase • Transcreener® UDP Assay UDP-glycosyltransferases • Transcreener® ADP2 FP Assay Kinases • [FP]2 cAMP

  7. Examples of AlphaScreen Applications: • Assay Target • AlphaScreen® cAMP Assay GPCR • AlphaScreen® SureFire® Assay GPCR • AlphaLISA® Immunoassay Kits Biological Markers (see below) • Biologics: IgA, IgG, IgM, CHO-P, MSO-P, E.Coli HCP, Residual Protein A • Angiogenesis: TNFα, VEGF, VEGFB, VEGFC, VEGFD • Cancer: AFP, CA125, CXCL11/I-TAC, EGF-R, EPO, ERBB2/HER2, IFNβ, MMP1, MMP2, MMP9, β-NGF, PSA • Cardiovascular: Myeloperoxidase, NT-proBNP, PCSK9, Plasminogen, Renin/Prorenin, tPA, ICAM-1 • Neurodegeneration: Aβ 1-40, Aβ 1-42, sAPPα, sAPPβ, Aβ 1-15/16 • Metabolic: Adiponectin, GH, GLP-1, IGF1, IGF2, Insulin, Leptin, Prolactin, Albumin • Virology: HIV p24 • Inflammation: CCL2/MCP1, CCL3/MIP-1α, CCL4/MIP-1β, COMP, CRP, CXCL10/IP-10, G-CSF, IFNα, IFNγ, IL1α, IL1β, IL2, IL3, IL4, IL5, IL6, IL7, IL8, IL10, IL12 (p70), IL13, IL17, IL18, CXCL1/GROα, CXCL9/MIG, IL15

  8. Enabling technologies covering the full spectrum of targets:

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