Chapter 4 Microbiological Contaminants. Waterworks Operations WQT 111 Lecture 4. E. coli are bad?. True False. Objectives. Compare virus (small), bacteria (medium), and protozoa (large) Review Total Coliform Rule. Go Over Total Coliform Analytical Procedures.
"E Coli H0157" is the bacterium that is the best known serotype of "Enterohemorrhagic E. Coli"and known by its more formal scientific name of Escherichia coli 0157:H7. Transmitted from undercooked hamburger meat and raw milk, it releases verotoxin, which causes inflammation of the lower gastrointestinal tract, often resulting in bloody bowel movements, potentially several other illnesses and/or death.
Membrane Filtration total coliform andTotal Coliforms (m-Endo broth)
Total Coliforms (http://dl.clackamas.cc.or.us/wqt111/unit-8-coliformtest.htm)
Fecal Coliforms (http://dl.clackamas.cc.or.us/wqt111/unit-8-coliformtest.htm)
MI-Media: Pure Culture of Escherichia coli ATCC 25922 with UV Light (http://www.whatman.com/products/?pageID=7.61.409.297)
Inoculate lauryl tryptose broth and incubate for 24h at 35oC
Gas or acidity
Transfer to BGBB
incubate 48 h at 35oC
No gas or acidity
24 h at 35oC
No gas produced
- test. Coliform group absent
Confirm as in (1)
Confirm as in (1)
Coliform group confirmed
Re-inoculate in fresh BGBB
Positive completed phase
Coliform bacteria- prior to analysis is 24 hours or less at 4 deg. C.? Microorganisms predominantly inhabiting the intestines of humans and other animals, but also occasionally found elsewhere. They include all aerobic and facultative anaerobic, Gram-negative, nonspore-forming, rod-shaped bacteria that ferment lactose with the production of gas. Also included are all bacteria that produce a dark, purplish-green metallic sheen by the membrane filter technique used for coliform identification.
# of organisms
Objective #4 on the syllabus (Understand the fundamentals of water microbiology, especially as it relates to waterborne infectious disease) has been met?