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Crimean Congo Hemorrhagic Fever- Pakistan Perspective

Crimean Congo Hemorrhagic Fever- Pakistan Perspective. National Institute of Health, Islamabad-Pakistan. Lab Methodologies used at NIH. Routine/Current Diagnosis: ELISA Kits used: BDSL IgM Capture ELISA RT-PCR Reference protocol:

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Crimean Congo Hemorrhagic Fever- Pakistan Perspective

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  1. Crimean Congo Hemorrhagic Fever- Pakistan Perspective National Institute of Health, Islamabad-Pakistan

  2. Lab Methodologies used at NIH Routine/Current Diagnosis: • ELISA Kits used: BDSL IgM Capture ELISA • RT-PCR Reference protocol: “Polymerase Chain Reaction for Diagnosis and Identification of Distinct Variants of Crimean-Congo Hemorrhagic Fever Virus in the United Arab Emirates. American Journal of Tropical Medicien and Hygiene, 55(2), 1996, pp. 190-196” • Genetic Sequencing: National Institute of Health, Pakistan holds the thorough capability including infrastructure and trained personnel but deficit of resources Discontinued methodologies: • Electron Microscopy • Inoculation in suckling mice • Fluorescent Microscopy

  3. Phylogenetic relationship of CCHF viruses detection during 2008-Quetta-Pakistan Outbreak 2008 PAK viruses (denoted by●) with Closest homology to: Asia Type-I Pakistan 1976, 2001 and 2002 viruses (denoted by ) as well as with Iran, Afghanistan Under Publication

  4. Future Needs for CCHF Lab Diagnosis at HIH • No recent changes to the lab diagnostic protocols • Need to establish a separate Core laboratory facility early service provision under improved standards • Establishment of standard guidelines for lab diagnosis

  5. Safety Precautions being used at NIH • BSL-II+ Laboratory with enhanced PPE • Separate Designated areas for sample receipt, processing and testing • Restricted personnel access • Waste management through Autoclaving and incineration

  6. Thanks

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