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E. &. O. O. &. Cultivation of viruses. Cultivation. To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation. Bacteria. Animal. Insect. Plant. Methods of animal virus cultivation. Organized tissue.

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E

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O

O

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Cultivation
Cultivation

To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.


Cultivation1
Cultivation

Bacteria

Animal

Insect

Plant


Methods of animal virus cultivation
Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.



Methods of animal virus cultivation1
Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Cell culture
Cell culture

  • Removal.

  • Homogenization.

  • Washing.

  • Trypsinization.

  • Washing.

  • Growing.

  • Inoculation.

  • Incubation.


Methods of animal virus cultivation2
Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Intracerebrally

Intraperitoneally

Intramuscularly

Intravenously

Intranasally


Types of cell culture
Types of cell culture

Primary

Permanent

Suspension

Diploid


Primary cell culture
Primary cell culture

A culture derived directly from a tissue


Diploid cell culture
Diploid cell culture

Subsequent cultures derived from primary cell culture.


Suspension cell culture
Suspension cell culture

A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.


Permanent cell line
Permanent cell line

A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely.


Cultivation of plant viruses
Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses1
Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Inoculation

Inoculation

Incubation

Incubation


Cultivation of plant viruses2
Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses3
Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.



Cultivation of bacterial viruses
Cultivation of bacterial viruses

1.On broth media

2. On solid medium


Virus + semisolid agar

Bacteria + solid agar




Criteria of purity
Criteria of purity

Chemical purity

Physical purity

Biological purity


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