Cultivation of viruses
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E. &. O. O. &. Cultivation of viruses. Cultivation. To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation. Bacteria. Animal. Insect. Plant. Methods of animal virus cultivation. Organized tissue.

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Cultivation of viruses

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E

&

O

O

&


Cultivation of viruses


Cultivation

To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.


Cultivation

Bacteria

Animal

Insect

Plant


Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Chick embryo technique


Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Cell culture

  • Removal.

  • Homogenization.

  • Washing.

  • Trypsinization.

  • Washing.

  • Growing.

  • Inoculation.

  • Incubation.


Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Intracerebrally

Intraperitoneally

Intramuscularly

Intravenously

Intranasally


Types of cell culture

Primary

Permanent

Suspension

Diploid


Primary cell culture

A culture derived directly from a tissue


Diploid cell culture

Subsequent cultures derived from primary cell culture.


Suspension cell culture

A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.


Permanent cell line

A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely.


Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Inoculation

Inoculation

Incubation

Incubation


Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Incubation


Cultivation of bacterial viruses

1.On broth media

2. On solid medium


Virus + semisolid agar

Bacteria + solid agar


Cultivation of Insect viruses


Thanks


Criteria of purity

Chemical purity

Physical purity

Biological purity


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