Cultivation of viruses
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E. &. O. O. &. Cultivation of viruses. Cultivation. To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation. Bacteria. Animal. Insect. Plant. Methods of animal virus cultivation. Organized tissue.

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Cultivation of viruses

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Cultivation of viruses

E

&

O

O

&


Cultivation of viruses

Cultivation of viruses


Cultivation

Cultivation

To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.


Cultivation1

Cultivation

Bacteria

Animal

Insect

Plant


Methods of animal virus cultivation

Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Cultivation of viruses

Chick embryo technique


Methods of animal virus cultivation1

Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Cell culture

Cell culture

  • Removal.

  • Homogenization.

  • Washing.

  • Trypsinization.

  • Washing.

  • Growing.

  • Inoculation.

  • Incubation.


Methods of animal virus cultivation2

Methods of animal virus cultivation

  • Organized tissue.

  • Cell culture.

  • Laboratory animals.


Cultivation of viruses

Intracerebrally

Intraperitoneally

Intramuscularly

Intravenously

Intranasally


Types of cell culture

Types of cell culture

Primary

Permanent

Suspension

Diploid


Primary cell culture

Primary cell culture

A culture derived directly from a tissue


Diploid cell culture

Diploid cell culture

Subsequent cultures derived from primary cell culture.


Suspension cell culture

Suspension cell culture

A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.


Permanent cell line

Permanent cell line

A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely.


Cultivation of plant viruses

Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses1

Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of viruses

Inoculation

Inoculation

Incubation

Incubation


Cultivation of plant viruses2

Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of plant viruses3

Cultivation of plant viruses

  • Protoplast and callus method.

  • Mechanical method.

  • Biological method.


Cultivation of viruses

Incubation


Cultivation of bacterial viruses

Cultivation of bacterial viruses

1.On broth media

2. On solid medium


Cultivation of viruses

Virus + semisolid agar

Bacteria + solid agar


Cultivation of insect viruses

Cultivation of Insect viruses


Cultivation of viruses

Thanks


Criteria of purity

Criteria of purity

Chemical purity

Physical purity

Biological purity


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