(1) Describe how the apparatus shown below is used to measure the rate of the enzyme catalysed decomposition of hydrogen peroxide. Side arm tube. Note:- the potato discs contain the enzyme catalase. Potato discs. Hydrogen peroxide.
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(1) Describe how the apparatus shown below is used to measure the rate of the enzyme
catalysed decomposition of hydrogen peroxide.
Side arm tube
Note:- the potato discs contain the enzyme catalase.
The effect of changes in pH and temperature on the decomposition of hydrogen peroxide (Unit 2 PPA 3)
The aim of the experiment is to show the effect of changing temperature or pH on the enzyme catalysed decomposition of hydrogen peroxide.
(1) When hydrogen peroxide decomposes it
gives off oxygen gas. The rate is
determined by counting the number of
bubbles of gas given off in 1 minute.
(2) Buffer solutions of differing pH values
(4, 7 and 10) are added to the
hydrogen peroxide solution.
(2) How can the effect of pH be investigated?
(3) The hydrogen peroxide solution is
warmed by placing the side arm tube in
a beaker of hot water until the desired
temperature is reached. Then the
potato discs are added.
(3) How can the effect of temperature be investigated?
(4) The graph shows the effect of temperature on the number of
bubbles of oxygen gas given off in 1 minute.
Describe how the activity of the enzyme is affected by
changes in temperature.
(4) The enzymeâ€™s activity is highest at a
temperature of about 37oC.
At temperatures higher, or lower
than this value the activity of the
enzyme decreases sharply.