(1)  Describe how the apparatus shown below is used to measure the rate of the enzyme
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(1) Describe how the apparatus shown below is used to measure the rate of the enzyme catalysed decomposition of hydrogen peroxide. Side arm tube. Note:- the potato discs contain the enzyme catalase. Potato discs. Hydrogen peroxide.

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(1) When hydrogen peroxide decomposes it gives off oxygen gas. The rate is

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1 when hydrogen peroxide decomposes it gives off oxygen gas the rate is

(1) Describe how the apparatus shown below is used to measure the rate of the enzyme

catalysed decomposition of hydrogen peroxide.

Side arm tube

Note:- the potato discs contain the enzyme catalase.

Potato discs

Hydrogen

peroxide

The effect of changes in pH and temperature on the decomposition of hydrogen peroxide (Unit 2 PPA 3)

The aim of the experiment is to show the effect of changing temperature or pH on the enzyme catalysed decomposition of hydrogen peroxide.

(1) When hydrogen peroxide decomposes it

gives off oxygen gas. The rate is

determined by counting the number of

bubbles of gas given off in 1 minute.

(2) Buffer solutions of differing pH values

(4, 7 and 10) are added to the

hydrogen peroxide solution.

(2) How can the effect of pH be investigated?

(3) The hydrogen peroxide solution is

warmed by placing the side arm tube in

a beaker of hot water until the desired

temperature is reached. Then the

potato discs are added.

(3) How can the effect of temperature be investigated?


1 when hydrogen peroxide decomposes it gives off oxygen gas the rate is

Number of

bubbles of

gas

20

15

10

5

Temperature (oC)

(4) The graph shows the effect of temperature on the number of

bubbles of oxygen gas given off in 1 minute.

Describe how the activity of the enzyme is affected by

changes in temperature.

(4) The enzyme’s activity is highest at a

temperature of about 37oC.

At temperatures higher, or lower

than this value the activity of the

enzyme decreases sharply.


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