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AfCD1

AfTD1. AfTI1. AfCI3. AfTD2. (a). AfTD3. (b). AfCD3. AfCD1. AfTD2. AfCD2. AfTD3. AfCI2. AfCD3. AfTI2. AfTD1. AfTI3. AfCD1. WC1. AfCI1. AfCD2. WC3. AfCI3. WT1. AfTI2. WT2. AfCI1. AfTI1. AfTI3. WT3. WC1. WT1. AfCI2. WC3. WT2. WC2. Supplemental Figure S1. (c).

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AfCD1

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  1. AfTD1 AfTI1 AfCI3 AfTD2 (a) AfTD3 (b) AfCD3 AfCD1 AfTD2 AfCD2 AfTD3 AfCI2 AfCD3 AfTI2 AfTD1 AfTI3 AfCD1 WC1 AfCI1 AfCD2 WC3 AfCI3 WT1 AfTI2 WT2 AfCI1 AfTI1 AfTI3 WT3 WC1 WT1 AfCI2 WC3 WT2 WC2 Supplemental Figure S1

  2. (c) AfCI1 AfCI2 AfCI3 AfCD1 AfCD2 AfCD3 WC3 AfTD2 AfTD3 AfTD1 AfTI2 AfTI3 AfTI1 WC1 WC2 WT2 WT3 WT1

  3. Supplemental Figure S2

  4. Supplemental Figure S3

  5. Supplemental material: • Supplemental Figure S1: Cluster analysis using the unweighted-pair group method for (a) total bacterial and (b) Pseudomonas and (c) Actinobacteria. AfCD, A. fulva from Caboclo Island extracted directly; AfCI, A. fulva from Caboclo Island extracted indirectly; AfTD, A. fulva from Tartaruga beach extracted directly; AfTI, A. fulva from Tartaruga beach extracted indirectly; SWC, surrounding seawater from Caboclo Island; SWT, surrounding seawater from Tartaruga beach. • Supplemental Figure S2: LIBSHUFF comparison plots of evolutionary distance by homologous coverage curves from direct (red line) compared to heterologous coverage curve from indirect (green line) 16S rRNA gene clone libraries (a) and homologous coverage curves for indirect (red line) compared to heterologous coverage curve direct (green line) 16S rRNA gene clone libraries (b). The blue line indicates the value of delta C (right y axis) for the original samples at each value of evolutionary distance. Purple line indicates the 95% of delta C for the randomized samples. The ranked values (p) of the delta C from the heterologous coverage are shown. • Supplemental Figure S3: Rarefaction curves of observed operational taxonomic units (OTU) at the “species” (97%, bold) and “genus” (95%, dotted) levels for partial bacterial 16S rRNA gene sequences retrieved from A. fulva derived DNA. The table on the upper-left side shows Chao1 richness estimations with their respective low and high confidence limits (95%), as well as the number of OTUs determined with DOTUR for each case evaluated.

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