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A C B D. HCN. Our basic premise: A Stem Cell Based Pacemaker requires a two cell functional syncytium.

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  1. A C B D

  2. HCN Our basic premise: A Stem Cell Based Pacemaker requires a two cell functional syncytium Hypothesis: A genetically engineered stem cell coupled to a heart cell can serve as a biological pacemaker. Gap Junction Stem cell Myocyte

  3. QD (red)+ Phase z 0 QD+hMSCs in needle track Intramuscular QD+hMSCs Epicardial border Tissue adhesive adsorbed to epicardium Imaging QD-hMSCs in histologic sections • Goals of study • Enumerate cells • 3-D reconstructions • Characterize distribution 1 mm Quantum dots Hoechst 33342 (nuclei)

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