1 / 7

Multi-Spectral Blending Presentation State

Multi-Spectral Blending Presentation State. Bas Hulsken. Philips Digital Pathology. August 26, 2011. Applications. 3+ channel true colour images: display multi-spectral visible light image sets with consistent color representation.

inoke
Download Presentation

Multi-Spectral Blending Presentation State

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Multi-Spectral Blending Presentation State Bas Hulsken Philips Digital Pathology August 26, 2011

  2. Applications • 3+ channel true colour images: display multi-spectral visible light image sets with consistent color representation. • Pseudo colour images of multi-channel fluorescence images: display multi-spectral pseudo colour images with consistent color representation. • Un-mixing of fluorescent channels for display • Un-mixing of fluorescent channels for measurements

  3. Un-mixing of fluorescent channels Linear un-mixing for known excitation, emission, light-source and filter spectra. Non-linear un-mixing methods are also available

  4. Current blending transformation model Limitations: • Blend only 2 channels • Only additive linear blending (opacity) (no un-mixing, no real ICC profile)

  5. Proposal: ICC based blending pipeline • Limitations • Maximum of 16 input channels • Maximum of 3 output channels, only suitable for display • Advantages • Flexible blending pipeline for linear and non-linear operations • Industry standard, full specification and reference implementations available Most advanced ICC pipeline (http://www.color.org/ICC1v42_2006-05.pdf)

  6. Using the blending pipeline • Case 1:display of multi-channel true colour visible light image. Program the pipeline directly by an ICC profile associated with the image acquisition device. • Case 2:display of (un-mixed) multi-channel fluorescence image. Specify the fluorophores absorption and emission spectra as well as the light source(s) spectra. The rendering software can then translate this internally to an ICC profile which programs the pipeline. • Case 3: un-mixing of multiple fluorescent channels for the purpose of quantitative measurements of individual fluorophore concentration. ??Same as 2, but only use L channel of PCS??

  7. Multi-Spectral Blending Presentation State based on ICC blending pipeline • Ability to consistently display ultra wide gamut true colour images • Ability to consistently display (un-mixed, linear, non-linear) multi-channel pseudo colour fluorescence images • All on the presentation side, so no modification of ‘raw’ captured data is required on the device side. • New powerful blending pipeline based on ICC, so reference specification and implementations are available.

More Related