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The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the

The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the Growth of Escherichia Coli Bacteria. Stephanie Close – Sterling Heights High School Claire St. Louis – Warren Mott High School. Why?. Antibiotic resistance Household remedies. Background.

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The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the

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  1. The Effect of Allium Sativum, Camellia Sinensis and Penicillin on the Growth of Escherichia Coli Bacteria Stephanie Close – Sterling Heights High School Claire St. Louis – Warren Mott High School

  2. Why? • Antibiotic resistance • Household remedies Close - St.Louis

  3. Background http://fineartamerica.com/art/photographs/petri+dish+culture+e.coli/all • Escherichia Coli • What is E.Coli bacteria? • Reproduction process Close - St.Louis

  4. Background http://en.wikipedia.org/wiki/Penicillin • Penicillin • What is penicillin? • How it kills bacteria Close - St.Louis

  5. Background http://carolcassara.com/2013/11/how-to-roast-garlic.html • Allium Sativum • What is Allium Sativum? • Antibacterial Agents Close - St.Louis

  6. Background http://www.wisegeek.com/what-is-camellia.htm# • Camellia Sinensis • What is Camellia Sinensis? • Antibacterial Agents Close - St.Louis

  7. Background http://www.antimicrobialtestlaboratories.com/Zone_of_Inhibition_Test_for_Antimicrobial_Activity.htm Zone of Inhibition Close - St.Louis

  8. Problem • To determine the effect of garlic powder, green tea, and penicillin on the growth of E.coli bacteria Close - St.Louis

  9. Hypothesis • Penicillin (g) will create the largest zone of inhibition (mm) while green tea (g) will create the smallest zone of inhibition (mm) Close - St.Louis

  10. Materials E.Coli Green Tea Garlic Powder Petri Dishes Coffee Filters Penicillin Close - St.Louis

  11. Procedures Close - St.Louis

  12. Data Close - St.Louis

  13. Observations • Large bacteria colonies • Green tea stain • Undissolved garlic powder Close - St.Louis

  14. Analysis Zone of Inhibition (mm) Close - St.Louis

  15. 2 Sample t-Test • Assumptions • 2 SRS from two distinct populations • Populations are independent • Normal Distribution • Population means and population standard deviations are not known t = t = Close - St.Louis

  16. Conclusion • Ho: μ1 = μ2 • Ha: μ1 ≠ μ2 • Garlic Powder vs. Penicillin • Failed to reject Ho • Green Tea vs. Penicillin • - Reject Ho Close - St.Louis

  17. Errors Treatment Solutions Placement of treatment Close - St.Louis

  18. Conclusion Hypothesis: Rejected Green tea created the largest zone of inhibition Close - St.Louis

  19. Further Research • Various factors • DOE test Close - St.Louis

  20. Recap • Problem • Hypothesis • Conclusion Close - St.Louis

  21. Resources • http://www.elmhurst.edu/~chm/vchembook/652penicillin.html • http://allicincenter.com/pdf/Allicin.pdf • http://www.webmd.com/vitamins-and-supplements/black-tea-uses-and-risks.com • http://www.westminster.edu/acad/sim/pdf/santibioticinhibitionofbacteria.pdf • http://mmbr.asm.org/content/65/2/232.full • http://pathmicro.med.sc.edu/fox/antibiotics1.htm • http://www.antibiotics-info.org/antibiotic-drugs.html • http://onlinelibrary.wiley.com/doi/10.1038.com • http://www.nlm.nih.gov/medlineplus/druginfo/meds/a682098.html • http://www.microbelibrary.org/component/resource/laboratory-test/3085-preparing-spread-plates-protocols.com • http://www.studymode.com/essays/The-Effects-Of-Black-Tea-On-1164976.html • http://www.all-sciencefairprojects.com/science_fair_pr ojects.html • http://www.ncbi.nlm.nih.gov/pubmed/23847105.com • http://www.ncbi.nlm.nih.gov/pubmed/15260343.com • http://www.drlwilson.com/articles/antibiotics.htm • http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html • http://community.middlebury.edu/~sontum/chemistry/students/atteridge/penicillin/penicillin.html • http://www.nutrimart.com/bulk/description/greentea.htm • http://examine.com/supplements/Green+Tea+Catechins/ • http://www.ncbi.nlm.nih.gov/pubmed/21827739 • http://healthyeating.sfgate.com/egcg-green-tea-8453.html • http://www.drhoffman.com/page.cfm/118 • http://www.amazon.com/Foods-Green-Extract-400mg-Vcaps/dp/B001DNV5CA • http://royalmajestea.com/basics.htm • http://community.middlebury.edu/~sontum/chemistry/students/atteridge/penicillin/penicillin.html • http://www.pbs.org/wgbh/evolution/library/10/4/l_104_03.html • http://www.teavivre.com/info/green-tea-history/ • http://www.westminster.edu/acad/sim/pdf/santibioticinhibition/ bacteria.pdf • http://mmbr.asm.org/content/65/2/232.full • http://pathmicro.med.sc.edu/fox/antibiotics1.htm • http://www.antibiotics-info.org/antibiotic-drugs.html • http://onlinelibrary.wiley.com/doi/10.1038.com Close - St.Louis

  22. Acknowledgments • Mr. Estapa • Mrs. Gravel • Bradley Rabquer Close - St.Louis

  23. Any Questions? Close - St.Louis

  24. Close - St.Louis

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