INVESTIGATION OF MONO-ISOPROPYLNAPHTHALENE, DI-ISOPROPYLNAPHTHALENE AND

1. 8 1 7 2 6 3 Sediment 5 4 Algae 2 1 Wax-10 MIPN 2,7 1,7 1,3 2,6 1,6 Ultra-2 1,4 1,5 DIPN 2,3 ① ④ Equity-5 ⑤ ② ⑥ TIPN ③ 1000m 0 INVESTIGATION OF MONO-ISOPROPYLNAPHTHALENE, DI-ISOPROPYLNAPHTHALENE AND TRI-ISOPROPYLNAPHTHALENEIN THE ENVIRONMENT AROUND THE PAPER RECYCLING PLANT Suzuki M, Matsumura C, Moriguchi Y, Nakano T Hyogo Prefectural Institute of Public Health and Environmental Sciences, 3-1-27 Yukihira-cho, Suma-ku, Kobe, Hyogo, 654-0037 Japan Introduction Sampling Mono-Isopropylnaphthalene ( MIPN ), Di-Isopropylnaphthalene( DIPN ) and Tri-Isopropylnaphthalene( TIPN ) are widely used for PCBs substitutes such as jet printer ink, pesticide solvent and so on. Air sampling at Point C Point A Ibo River Point B They are concerned to have harmful influences to health and environment because of their high persistent and accumulating properties, but their fate in the environment had not been illustrated. Their optimum analytical methods for environmental samples had not been developed. Point D Point C The Paper Recycling Plant MIPN DIPN Point D’ TIPN (1,3-DIPN) (1,3,5-TIPN) (1-MIPN) Point E purpose Wastewater from Paper Recycling plant 1. Development of their simultaneous analytical methods for the water, the air, the sediment and the biological samples. 2. Investigation of their concentration and isomer fingerprints in the environmental samples around the paper recycling plant. Point F Hyogo Pref. Ibo river at Point F GC/MS Condition Off the coast of Himeji GC/MS： Agilent6890N/JMS-Q1000GC Point G Column：Supelcowax-10（Polyethleneglycol） (0.32mm×30m×0.25μm) MS GC Interface：240℃ Carrier　Gas：He（1mL/min） Results Ion Source：210℃ Injection： Split less（Purge ON　1.5min） Ionization：300μA, 70eV Injection Volume　： 2μL Detection Mode：SIM Injection Temp.： 270℃ Monitor Ion: 254、212、170、156、142 DIPN TIPN Oven：50℃（1）→20℃/min→100℃→5℃ /min→210℃→20℃/min→270℃（1） HCB-13C6（ Internal Standard ）: 290, 255 Sediment Algae Sediment Algae Water N.A. N.A. N.A. N.D. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. N.A. Fig.2 Separated DIPN isomer peaks by columns. Fig.1 The chromatogram of MIPN, DIPN and TIPN standard. N.A. N.A. N.D. ・DIPN isomers peaks was rather separated by using polar column than medium polar one. N.A. Sea bass N.A. ・MIPN and DIPN isomers can be assigned and 6 TIPN isomers peaks was separated. Air News paper Analytical procedure Water sample Shaking or mixing (the roof of our institute) Concentrated to 200μL Fig.3 The concentration of DIPN and TIPN in samples. 1L water sample Extracted by 10mL Hexane Clean up by sulfuric acid Analyzed by GC/MS ＋NaCl 30g Sediment and Biological sample Concentrated to 200μL Analyzed by GC/MS 20g sample methanol-hexane partition Cleaned up Extracted by alkaline degradation with 50mL KOH/MeOH for about 12 hours. Fig.4 The fingerprints of DIPN isomers in samples. Y axis indicates a ratio to 2,6-DIPN. by the silica gel containing 5% water or florisil or sulfuric acid N.D. Air sample Conclusion 1. The simultaneous analytical methods of MIPN, DIPN and TIPN in the water, the air, the sediment and the biological sampleswere developed. Concentrated to 200μL Analyzed by GC/MS 2. DIPN and TIPN were detected from the water, the sediment, the air and the biological samples and MIPN was not detected all samples. Sampling 0.5 m3 ~ 10m3 air were introduced into PS-Air Extracted by 30mL DCM 3. The wastewater and the exhaust from the paper recycling plant were one of loading sources of DIPN. Recovery 4. It was considered that there was another pollution resource of DIPN and TIPN in the Ibo River because high concentration of TIPN and the unusual fingerprint of DIPN isomers were detected from the sediment which was not influenced by the wastewater from the recycled paper factory. 5. It was considered that DIPN in sea bass was metabolized becausethe rates of decomposition were different by isomers. amount：20ng or 40ng Detection limit (water) References Unit: ng/L Brzozowski R, Skupinski W, Jamroz H, M, Skarzynski M, Otwinowska H. Journal of Chromatography A 2002;946